当前位置： SCI文献检索 > MOLECULAR AND BIOCHEMICAL PARASITOLOGY期刊下所有文献 > An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases.

An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases.

Abstract：

:We have determined the sequence of a cDNA (Tt34c1) encoding a Trypanosoma cruzi trypomastigote stage-specific 85-kDa surface glycoprotein (gp85). Within the peptide sequence of Tt34c1 are two 8-amino acid motifs, Ser-X-Asp-X-Gly-X-Thr-Trp, that are characteristic of bacterial neuraminidases. Analysis of the Tt34c1 sequence predicts the presence of an amino-terminal signal sequence and a hydrophobic carboxy-terminus that is probably replaced by a glycosyl phosphatidylinositol membrane anchor. Gp85 is encoded by an extensive multigene family that is distributed throughout the genome and can be divided into subsets on the basis of oligonucleotide hybridisation patterns. By sequencing products of polymerase chain reaction (PCR) amplification of the 5' end of trypomastigote gp85 mRNA we show that multiple copies of the gene family are transcribed simultaneously in a trypanosome population. Comparison of the sequence of the PCR clones and another gp85 cDNA showed a highly conserved region 5' of the first methionine extending 180 nt into the coding sequence. Insertions and point mutations were observable outside these homologous sequences demonstrating the variant nature of the gp85 mRNAs.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Takle GB,Cross GA

doi

10.1016/0166-6851(91)90114-l

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

185-98

issue

2

eissn

0166-6851

issn

1872-9428

pii

0166-6851(91)90114-L

journal_volume

48

pub_type

杂志文章

相关文献

MOLECULAR AND BIOCHEMICAL PARASITOLOGY文献大全
  • Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences.

    abstract：:Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111260

    authors： Arbabi M,Hooshyar H,Lotfinia M,Bakhshi MA

    更新日期：2020-03-01 00:00:00

  • Transcribing and replicating particles in a double-stranded RNA virus from Leishmania.

    abstract：:During the replicative cycle of many double-stranded RNA viruses, transcription of particles with a double-stranded RNA genome alternates with replication of particles containing a single-stranded genome. In virions infecting some strains of Leishmania guyanensis the putative transcriptase and replicase activities of ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90053-m

    authors： Weeks RS,Patterson JL,Stuart K,Widmer G

    更新日期：1992-06-01 00:00:00

  • Purification and properties of Plasmodium falciparum malate dehydrogenase.

    abstract：:Asexual intraerythrocytic Plasmodium falciparum were shown to have a single isoenzyme of malate dehydrogenase. This malate dehydrogenase was purified to apparent homogeneity using a three-step purification protocol. The parasite malate dehydrogenase had an apparent subunit molecular weight of 32 kDa, a pH optimum of 7...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90240-k

    authors： Lang-Unnasch N

    更新日期：1992-01-01 00:00:00

  • The divergent N-terminal domain of Tim17 is critical for its assembly in the TIM complex in Trypanosoma brucei.

    abstract：:Trypanosoma brucei Tim17(TbTim17), the single member of the Tim17/23/22 protein family, is an essential component of the translocase of the mitochondrial inner membrane (TIM). In spite of the conserved secondary structure, the primary sequence of TbTim17, particularly the N-terminal hydrophilic region, is significantl...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2017.09.003

    authors： Weems E,Singha UK,Smith JT,Chaudhuri M

    更新日期：2017-12-01 00:00:00

  • Alpha and beta subunits of pyruvate dehydrogenase E1 from the microsporidian Nosema locustae: mitochondrion-derived carbon metabolism in microsporidia.

    abstract：:Microsporidia are highly adapted eukaryotic intracellular parasites that infect a variety of animals. Microsporidia contain no recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsp...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00356-5

    authors： Fast NM,Keeling PJ

    更新日期：2001-10-01 00:00:00

  • The proteasome-ubiquitin pathway in the Schistosoma mansoni egg has development- and morphology-specific characteristics.

    abstract：:Schistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2010.10.005

    authors： Mathieson W,Castro-Borges W,Wilson RA

    更新日期：2011-02-01 00:00:00

  • The ecdysone receptor (EcR) is a major regulator of tissue development and growth in the marine salmonid ectoparasite, Lepeophtheirus salmonis (Copepoda, Caligidae).

    abstract：:The function of the ecdysone receptor (EcR) during development and molting has been thoroughly investigated in some arthropods such as insects but rarely in crustacean copepods such as the salmon louse Lepeophtheirus salmonis (L. salmonis) (Copepoda, Caligidae). The salmon louse is an ectoparasite on Atlantic salmon t...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2016.06.007

    authors： Sandlund L,Nilsen F,Male R,Dalvin S

    更新日期：2016-08-01 00:00:00

  • Isolation and substrate specificity of an adenine nucleoside phosphorylase from adult Schistosoma mansoni.

    abstract：:An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC 2.4.2.1). ANP is specific for adenine nucleosides...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2014.04.008

    authors： Savarese TM,El Kouni MH

    更新日期：2014-03-01 00:00:00

  • Mechanisms of respiration and phosphorylation in Ascaris muscle mitochondria.

    abstract：:In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(80)90002-x

    authors： Köhler P,Bachmann R

    更新日期：1980-04-01 00:00:00

  • Expression profiling of the Leishmania life cycle: cDNA arrays identify developmentally regulated genes present but not annotated in the genome.

    abstract：:As genomic sequencing of Leishmania nears completion, functional analyses that provide a global genetic perspective on biological processes are important. Despite polycistronic transcription, RNA transcript abundance can be measured using microarrays. To provide a resource to evaluate cDNA arrays, we undertook 5' expr...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2004.03.004

    authors： Almeida R,Gilmartin BJ,McCann SH,Norrish A,Ivens AC,Lawson D,Levick MP,Smith DF,Dyall SD,Vetrie D,Freeman TC,Coulson RM,Sampaio I,Schneider H,Blackwell JM

    更新日期：2004-07-01 00:00:00

  • Repeat structures in a Plasmodium falciparum protein (MESA) that binds human erythrocyte protein 4.1.

    abstract：:The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90231-8

    authors： Coppel RL

    更新日期：1992-02-01 00:00:00

  • Chemical modification of fructose bisphosphate aldolase from Trypanosoma brucei compared to aldolase from rabbit muscle and Staphylococcus aureus.

    abstract：:Chemical modifications of Class I aldolases from Trypanosoma brucei, rabbit muscle and Staphylococcus aureus with carboxypeptidase A, glyceraldehyde 3-phosphate and cysteine-specific reagents revealed the following differences between the three homologous enzymes. Aldolase from S. aureus was not affected by any of the...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90143-t

    authors： Callens M,Opperdoes FR

    更新日期：1991-07-01 00:00:00

  • Characterisation of benzimidazole binding with recombinant tubulin from Giardia duodenalis, Encephalitozoon intestinalis, and Cryptosporidium parvum.

    abstract：:The binding kinetics of several benzimidazole compounds were determined with recombinant tubulin from benzimidazole-sensitive and -insensitive organisms. This study utilised the naturally occurring high efficacy of the benzimidazoles for the parasitic protozoa Giardia duodenalis and Encephalitozoon intestinalis, and l...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2004.08.001

    authors： MacDonald LM,Armson A,Thompson AR,Reynoldson JA

    更新日期：2004-11-01 00:00:00

  • The interaction of artemisinin with malarial hemozoin.

    abstract：:[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)90014-0

    authors： Hong YL,Yang YZ,Meshnick SR

    更新日期：1994-01-01 00:00:00

  • Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes.

    abstract：:The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)90038-8

    authors： Pouvelle B,Gormley JA,Taraschi TF

    更新日期：1994-07-01 00:00:00

  • Characterization of the eg95 gene family in the G6 genotype of Echinococcus granulosus.

    abstract：:Cystic echinococcosis in humans and livestock animals is caused by infection with the cestode parasite Echinococcus granulosus. A number of genotypes of the parasite (designated G1-G10) are known to exist, with the genotype cluster G1-G3 and genotype G6 being responsible for the majority of humans infections. A recomb...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2012.02.005

    authors： Alvarez Rojas CA,Gauci CG,Nolan MJ,Harandi MF,Lightowlers MW

    更新日期：2012-06-01 00:00:00

  • Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress.

    abstract：:Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In th...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111277

    authors： Nava S,Sadiqova A,Castellanos-Gonzalez A,White AC Jr

    更新日期：2020-05-01 00:00:00

  • Analysis of the sequences flanking the translational start sites of Plasmodium falciparum.

    abstract：:The 5' and 3' regions adjacent to the initiation codon in 22 Plasmodium falciparum sequences were examined. A 5' consensus sequence (AAAA/ATG) was found. Although P. falciparum non-translated DNA is A-rich, A occurred significantly more frequently in the 4 positions preceding the initiation ATG than in adjacent non-tr...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(90)90112-y

    authors： Saul A,Battistutta D

    更新日期：1990-08-01 00:00:00

  • Identification of Toxoplasma TgPH1, a pleckstrin homology domain-containing protein that binds to the phosphoinositide PI(3,5)P2.

    abstract：:The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2016.03.011

    authors： Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

    更新日期：2016-05-01 00:00:00

  • Toxoplasma gondii toxolysin 4 is an extensively processed putative metalloproteinase secreted from micronemes.

    abstract：:Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoites store TLN4 in the micronemes and secrete it in response to elevated calcium, ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2011.01.009

    authors： Laliberté J,Carruthers VB

    更新日期：2011-05-01 00:00:00

  • DNA sequence of Crithidia fasciculata kinetoplast minicircles.

    abstract：:Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(87)90032-6

    authors： Sugisaki H,Ray DS

    更新日期：1987-04-01 00:00:00

  • cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite.

    abstract：:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(95)00098-l

    authors： el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

    更新日期：1995-07-01 00:00:00

  • Trypanosoma cruzi response to the oxidative stress generated by hydrogen peroxide.

    abstract：:As an intracellular parasite, Trypanosoma cruzi is exposed to reactive oxygen species. The study of the proteins involved in the hydroperoxide detoxification cascade, tryparedoxin peroxidase included, may lead to the development of a more specific chemotherapy for Chagas'disease. In this work, the involvement of TcCPX...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2003.08.011

    authors： Finzi JK,Chiavegatto CW,Corat KF,Lopez JA,Cabrera OG,Mielniczki-Pereira AA,Colli W,Alves MJ,Gadelha FR

    更新日期：2004-01-01 00:00:00

  • The free amino acid pool in Leishmania tropica promastigotes.

    abstract：:The principal constituents of the free amino acid pool in Leishmania tropica promastigotes include alanine, glycine, serine, threonine, alpha-aminobutyric acid and ethanolamine. Changes in levels of the pool constituents during starvation, nutrient supplementation and treatment with a metabolic inhibitor (sodium fluor...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(83)90056-7

    authors： Simon MW,Jayasimhulu K,Mukkada AJ

    更新日期：1983-09-01 00:00:00

  • The chloramphenicol acetyltransferase vector as a tool for stable tagging of Neospora caninum.

    abstract：:Neospora caninum is an obligate intracellular Apicomplexa, a phylum where one of the current methods for functional studies relies on molecular genetic tools. For Toxoplasma gondii, the first method described, in 1993, was based on resistance against chloramphenicol. As in T. gondii, we developed a vector constituted ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2014.08.001

    authors： Pereira LM,Yatsuda AP

    更新日期：2014-09-01 00:00:00

  • Demonstration of glycosomes (microbodies) in the Bodonid flagellate Trypanoplasma borelli (Protozoa, Kinetoplastida).

    abstract：:Homogenates of Trypanoplasma borelli were subjected to subcellular fractionation by sequential differential and isopycnic centrifugation in sucrose. Glycerol-3-phosphate dehydrogenase and the glycolytic enzymes, glucosephosphate isomerase and triosephosphate isomerase, as well as the peroxisomal marker enzyme catalase...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(88)90108-9

    authors： Opperdoes FR,Nohynkova E,Van Schaftingen E,Lambeir AM,Veenhuis M,Van Roy J

    更新日期：1988-08-01 00:00:00

  • Zinc finger nuclease technology: A stable tool for high efficiency transformation in bloodstream form T. brucei.

    abstract：:In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2017.02.002

    authors： Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

    更新日期：2017-04-01 00:00:00

  • The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula.

    abstract：:This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose con...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90202-u

    authors： Horemans AM,Tielens AG,van den Bergh SG

    更新日期：1992-03-01 00:00:00

  • Mediation of Trypanosoma cruzi invasion by heparan sulfate receptors on host cells and penetrin counter-receptors on the trypanosomes.

    abstract：:Trypanosoma cruzi attaches and invades a large variety of mammalian cells by receptor-mediated interactions, one of them involving the binding of parasite trans-sialidase to host sialyl receptors. Three proteoglycan-deficient mutants of Chinese hamster ovary (CHO) cells were used to probe the role of host heparin and ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)90116-3

    authors： Herrera EM,Ming M,Ortega-Barria E,Pereira ME

    更新日期：1994-05-01 00:00:00

  • Respiration of Tritrichomonas foetus: components detected in hydrogenosomes and in intact cells by electron paramagnetic resonance spectrometry.

    abstract：:Electron paramagnetic resonance spectrometry (EPR) techniques revealed a complex set of redox components in intact respiring cells of Tritrichomonas foetus, an aerotolerant anaerobic protozoon which lacks mitochondria. One of the two main systems of terminal respiration of this organism is the hydrogenosomal system, w...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(80)90047-x

    authors： Ohnishi T,Lloyd D,Lindmark DG,Müller M

    更新日期：1980-10-01 00:00:00