TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei.

Abstract:

:The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contrast, as shown in this study, knockdown of TbARF1 by RNA interference has no effect on fluid-phase endocytosis in the insect stage of the parasite. The protein remains essential for the viability of these procyclic cells but the major effect of TbARF1-depletion is enlargement of the lysosome. Our data indicate that protein trafficking and lysosomal function are differentially regulated by multiple factors, including TbARF1, during progression through the T. brucei lifecycle.

journal_name

Mol Biochem Parasitol

authors

Price HP,Stark M,Smith B,Smith DF

doi

10.1016/j.molbiopara.2007.06.009

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

123-7

issue

2

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(07)00167-3

journal_volume

155

pub_type

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