A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion.


:Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence analysis of the gene encoding GP900, a previously identified > 900 kDa glycoprotein, predicts a mucin-like glycoprotein composed of distal cysteine-rich domains separated by polythreonine domains and a large membrane proximal N-glycosylated core region. A trinucleotide repeat composed predominantly of the triplet ACA encodes the threonine domains. GP900 is stored in micronemes prior to appearance on the surface of invasive forms. The concentration of native GP900 which inhibits 50% (IC50) of invasion in vitro is low picomolar; the IC50 for a recombinant cysteine rich-domain is low nanomolar. These observations indicate that GP900 is a parasite ligand for a host receptor involved in attachment/invasion and suggest that immunotherapy or chemotherapy directed against GP900 may be feasible.


Mol Biochem Parasitol


Barnes DA,Bonnin A,Huang JX,Gousset L,Wu J,Gut J,Doyle P,Dubremetz JF,Ward H,Petersen C




Has Abstract


1998-10-30 00:00:00














  • Stage-specific expression of the Leishmania mexicana paraflagellar rod protein PFR-2.

    abstract::A screen for Leishmania mexicana genes encoding promastigote-specific flagellar proteins resulted in isolation of genes encoding the major components of the paraflagellar rod. One of these, PFR-2, was characterized extensively. PFR-2 genes are present in the genome as a tandem array of three genes designated PFR-2A, P...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Moore LL,Santrich C,LeBowitz JH

    更新日期:1996-10-01 00:00:00

  • The effect of over-expression of the alternative oxidase in the procyclic forms of Trypanosoma brucei.

    abstract::Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Walker R Jr,Saha L,Hill GC,Chaudhuri M

    更新日期:2005-02-01 00:00:00

  • Plasmodium falciparum biosynthesizes sulfoglycosphingolipids.

    abstract::Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, st...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Landoni M,Duschak VG,Peres VJ,Nonami H,Erra-Balsells R,Katzin AM,Couto AS

    更新日期:2007-07-01 00:00:00

  • An exported protein of Plasmodium falciparum is synthesized as an integral membrane protein.

    abstract::Exp-1 is an antigen of Plasmodium falciparum which is transported from the parasite cell to the membrane of the parasitophorous vacuole and to membranous compartments in the erythrocyte. To investigate how this protein is transported, we studied the synthesis and membrane translocation of exp-1 in a cell-free system. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Günther K,Tümmler M,Arnold HH,Ridley R,Goman M,Scaife JG,Lingelbach K

    更新日期:1991-05-01 00:00:00

  • Base J glucosyltransferase does not regulate the sequence specificity of J synthesis in trypanosomatid telomeric DNA.

    abstract::Telomeric DNA of trypanosomatids possesses a modified thymine base, called base J, that is synthesized in a two-step process; the base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU) and a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). To examine the importa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bullard W,Cliffe L,Wang P,Wang Y,Sabatini R

    更新日期:2015-12-01 00:00:00

  • Characterization of phosphodiesterase-5 as a surface protein in the tegument of Schistosoma mansoni.

    abstract::Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable me...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rofatto HK,Tararam CA,Borges WC,Wilson RA,Leite LC,Farias LP

    更新日期:2009-07-01 00:00:00

  • Cloning and sequencing of an hsp70 gene of Schistosoma mansoni.

    abstract::Schistosomes have a complex life cycle (vertebrate and molluscan hosts as well as larvae living freely in water) in which they are exposed to different environments and temperatures (20 degrees C - 37 degrees C). Since heat shock genes are activated in response to stress and during development [1], it is of interest t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Neumann S,Ziv E,Lantner F,Schechter I

    更新日期:1992-12-01 00:00:00

  • Purification and characterization of three larval excretory-secretory proteins of Dirofilaria immitis.

    abstract::Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Frank GR,Grieve RB

    更新日期:1996-01-01 00:00:00

  • Patterns of polymorphism in genomic regions flanking three highly polymorphic surface antigens in Plasmodium falciparum.

    abstract::Many surface antigens of the human malaria parasite Plasmodium falciparum show extraordinary diversity, with different alleles being so divergent as to be unalignable in some coding regions. To better understand the population history and modes of selection on such loci, we sequenced genomic regions flanking the highl...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Amodu OK,Hartl DL,Roy SW

    更新日期:2008-05-01 00:00:00

  • Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries.

    abstract::We have generated 2771 expressed sequence tags (ESTs) from two cDNA libraries of Trypanosoma cruzi CL-Brener. The libraries were constructed from trypomastigote and amastigotes, using a spliced leader primer to synthesize the cDNA second strand, thus selecting for full-length cDNAs. Since the libraries were not normal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Agüero F,Abdellah KB,Tekiel V,Sánchez DO,González A

    更新日期:2004-08-01 00:00:00

  • Preference of A/T ending codons in mitochondrial ATP6 gene under phylum Platyhelminthes: Codon usage of ATP6 gene in Platyhelminthes.

    abstract::Unequal usage of synonymous codons in the gene transcript for an amino acid is known as codon usage bias (CUB). It is a unique property of gene as well as genome. Mutation and natural selection are known to be the major factors that influence CUB. Other factors encompass gene expression level, GC content, codon positi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mazumder GA,Uddin A,Chakraborty S

    更新日期:2018-10-01 00:00:00

  • Upstream regulatory elements controlling expression of the Entamoeba histolytica lectin.

    abstract::Entamoeba histolytica genomic organization and putative promoter elements appear to be distinct from both metazoan and better characterized protozoan organisms. The recent development of DNA-mediated transfection for E. histolytica enabled characterization of cis-acting promoter elements required for gene expression. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Purdy JE,Pho LT,Mann BJ,Petri WA Jr

    更新日期:1996-06-01 00:00:00

  • Insights into blood feeding by schistosomes from a proteomic analysis of worm vomitus.

    abstract::Whilst the schistosome tegument has been intensively studied there is little information about processes in the gut, the other major interface with the bloodstream, apart from the well characterised cascade of proteases involved in haemoglobin digestion. To gain insights into gut function we undertook a proteomic anal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hall SL,Braschi S,Truscott M,Mathieson W,Cesari IM,Wilson RA

    更新日期:2011-09-01 00:00:00

  • Toxoplasma gondii toxolysin 4 is an extensively processed putative metalloproteinase secreted from micronemes.

    abstract::Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoites store TLN4 in the micronemes and secrete it in response to elevated calcium, ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Laliberté J,Carruthers VB

    更新日期:2011-05-01 00:00:00

  • The biochemical and immunochemical characterisation of the 30 kilodalton surface antigen of Brugia pahangi.

    abstract::The major surface antigen (30 kDa) of Brugia pahangi has been characterised by a number of biochemical and immunochemical means. The 30 kDa polypeptide is a glycoprotein which can be extracted from the worm surface by homogenization in the absence of detergents. The 30 kDa polypeptide can be metabolically labelled wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Devaney E

    更新日期:1988-01-01 00:00:00

  • Multimerization of the Toxoplasma gondii MIC2 integrin-like A-domain is required for binding to heparin and human cells.

    abstract::Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis in humans and animals. To invade host cells, T. gondii deploys the contents of its apically oriented secretory organelles that include the micronemes. Contained within the micronemes are proteins that possess adhesive motifs resembling t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Harper JM,Hoff EF,Carruthers VB

    更新日期:2004-04-01 00:00:00

  • Glycogen metabolism in Schistosoma mansoni worms after their isolation from the host.

    abstract::Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tielens AG,van den Bergh SG

    更新日期:1987-07-01 00:00:00

  • The presence of galactofuranose and ribose units in asparagine-linked oligosaccharides of the digenetic trypanosomatid Endotrypanum schaudinni.

    abstract::It was found that the digenetic trypanosomatid Endotrypanum schaudinni transferred Man7GlcNAc2 in protein N-glycosylation. Endo-beta-N-acetylglucosaminidase H-sensitive oligosaccharides were identified as Man7GlcNAc2, Man6GlcNAc2, Rib1Man6GlcNAc2 and/or Gal1Man6GlcNAc2, Man5GlcNAc containing two galactose or ribose un...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Merello S,Xavier MT,Parodi AJ

    更新日期:1995-01-01 00:00:00

  • Characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time PCR.

    abstract::Human hookworms (Ancylostoma duodenale, Necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. Mass drug administration (MDA) with benzimidazole (BZ) anthelmintics has the potential to greatly reduce morbidity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

    更新日期:2007-12-01 00:00:00

  • Purification and partial characterization of an unusual protein of Plasmodium falciparum: histidine-rich protein II.

    abstract::The human malarial parasite Plasmodium falciparum secretes a histidine-rich protein (HRP-II) from infected erythrocytes. HRP-II has a very high content of histidine (H) (34%), alanine (A) (37%) and aspartic acid (D) (10%) and many contiguous repeats of the sequences AHH and AHHAAD. The histidine content of the protein...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Panton LJ,McPhie P,Maloy WL,Wellems TE,Taylor DW,Howard RJ

    更新日期:1989-06-15 00:00:00

  • RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs.

    abstract::Schistosoma mansoni leucine aminopeptidase (LAP) is thought to play a central role in hatching of the miracidium from the schistosome egg. We identified two discrete LAPs genes in the S. mansoni genome, and their orthologs in S. japonicum. The similarities in sequence and exon/intron structure of the two genes, LAP1 a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,Dalton JP,Tort JF,Brindley PJ

    更新日期:2009-10-01 00:00:00

  • A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting.

    abstract::We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Brisse S,Barnabé C,Bañuls AL,Sidibé I,Noël S,Tibayrenc M

    更新日期:1998-05-01 00:00:00

  • Histone mediated gene activation in Toxoplasma gondii.

    abstract::DNA processes, including transcriptional activation, are largely governed by histone modifications. For most eukaryotes, chromatin-remodeling machines have been identified that move, replace, or decorate histones. Recent studies have shed some important light on similar machinery operating in apicomplexan parasites, i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Sullivan WJ Jr,Hakimi MA

    更新日期:2006-08-01 00:00:00

  • Isolation and characterisation of ribosomal RNA from the human malaria parasite Plasmodium falciparum.

    abstract::Ribosomal RNA isolated from the malaria parasite Plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 X 10(6) and 0.78 +/- 0.02 X 10(6) present in equimolar quantities. Their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hyde JE,Zolg JW,Scaife JG

    更新日期:1981-12-31 00:00:00

  • A tubulin-related 55 kilodalton surface antigen recognized by different Trypanosoma cruzi stage-specific monoclonal antibodies from infected mice.

    abstract::Thirteen monoclonal antibodies (MAbs) specific for the membrane of live Trypanosoma cruzi have been obtained from BALB/c infected mice. Most of them had greater avidity for intact than for disrupted parasites. According to the staining by indirect immunofluorescence of the different live developmental stages of the pa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alcina A,Fresno M

    更新日期:1988-06-01 00:00:00

  • Real-time PCR for sexing Schistosoma mansoni cercariae.

    abstract::The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

    更新日期:2016-01-01 00:00:00

  • Repeat structures in a Plasmodium falciparum protein (MESA) that binds human erythrocyte protein 4.1.

    abstract::The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Coppel RL

    更新日期:1992-02-01 00:00:00

  • Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces.

    abstract::Cystic hydatid disease (CHD) is caused by infection with Echinococcus granulosus metacestodes and affects humans and livestock. Proteins secreted or excreted by protoscoleces, pre-adult worms found in the metacestode, are thought to play fundamental roles in the host-parasite relationship. In this work, we performed a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Virginio VG,Monteiro KM,Drumond F,de Carvalho MO,Vargas DM,Zaha A,Ferreira HB

    更新日期:2012-05-01 00:00:00

  • Similarity of a 16.5kDa tegumental protein of the human liver fluke Opisthorchis viverrini to nematode cytoplasmic motility protein.

    abstract::Opisthorchis viverrini is the causative agent of human opisthorchiasis in Thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. In this work we have molecularly characterized the first member of a protein family carrying two DM9 repeats in this parasite (...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Labbunruang N,Phadungsil W,Tesana S,Smooker PM,Grams R

    更新日期:2016-05-01 00:00:00

  • Conditional removal of selectable markers in Trypanosoma cruzi using a site-specific recombination tool: proof of concept.

    abstract::The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kangussu-Marcolino MM,Cunha AP,Avila AR,Herman JP,DaRocha WD

    更新日期:2014-12-01 00:00:00