A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion.

abstract：:Schistosoma mansoni leucine aminopeptidase (LAP) is thought to play a central role in hatching of the miracidium from the schistosome egg. We identified two discrete LAPs genes in the S. mansoni genome, and their orthologs in S. japonicum. The similarities in sequence and exon/intron structure of the two genes, LAP1 a...

journal_title：Molecular and biochemical parasitology

authors： Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,Dalton JP,Tort JF,Brindley PJ

更新日期：2009-10-01 00:00:00

abstract：:The cysteine proteinase B of Leishmania parasites is an important virulence factor. In this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative agent of mucocutaneous leishmaniosis. The mature region of the recombinant CPB shares a high p...

journal_title：Molecular and biochemical parasitology

authors： Lanfranco MF,Loayza-Muro R,Clark D,Núñez R,Zavaleta AI,Jimenez M,Meldal M,Coombs GH,Mottram JC,Izidoro M,Juliano MA,Juliano L,Arévalo J

更新日期：2008-10-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

journal_title：Molecular and biochemical parasitology

authors： Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

更新日期：2016-05-01 00:00:00

abstract：:Both the alpha- and beta-tubulin genes of Leishmania enriettii are encoded by mRNA of 2.0-2.2 kb in length. We have shown previously that the alpha- and beta-tubulin genes are arranged in separate, tandem repeats of 2 and 4 kb, respectively, and now report the mapping of mature mRNA onto these cloned genes. Here we sh...

journal_title：Molecular and biochemical parasitology

authors： Landfear SM,Wirth DF

更新日期：1985-04-01 00:00:00

abstract：:Giardia lamblia undergoes surface antigenic variation. The variant-specific surface proteins (VSPs) of isolate WB are cysteine-rich, can vary dramatically in size, contain Cys-X-X-Cys motifs, and are differentially expressed. GS/M(H7) is a Giardia clone from a different isolate which expresses a VSP epitope not found ...

journal_title：Molecular and biochemical parasitology

authors： Nash TE,Mowatt MR

更新日期：1992-04-01 00:00:00

abstract：:We have demonstrated that bradyzoites return to the tissue-cyst stage by a developmental pathway that is indistinguishable from that initiated by sporozoites. Mature bradyzoites, like sporozoites from oocysts, were non-proliferative as they contained uniform 1N DNA contents, and replication occurred only in parasites ...

journal_title：Molecular and biochemical parasitology

authors： Radke JR,Guerini MN,Jerome M,White MW

更新日期：2003-10-01 00:00:00

abstract：:In silico analyses of Leishmania spp. genome data are a powerful resource to improve the understanding of these pathogens' biology. Trypanosomatids such as Leishmania spp. have their protein-coding genes grouped in long polycistronic units of functionally unrelated genes. The control of gene expression happens by a va...

journal_title：Molecular and biochemical parasitology

authors： Vasconcelos EJ,Terrão MC,Ruiz JC,Vêncio RZ,Cruz AK

更新日期：2012-06-01 00:00:00

abstract：:Growth inhibition and radioisotope incorporation studies with allopurinol (4-hydroxypyrazolo(3,4-d)pyrimidine) have shown that the African trypanosomes are biologically and biochemically similar to Leishmania spp. and Trypanosoma cruzi with respect to their response to this compound. These organisms, as a group, share...

journal_title：Molecular and biochemical parasitology

authors： Berens RL,Marr JJ,Brun R

更新日期：1980-04-01 00:00:00

abstract：:The binding of tritiated benzimidazoles (BZs)-albendazole, parbendazole, oxibendazole, mebendazole, oxfendazole and fenbendazole-to crude tubulin extracts from BZ-susceptible and -resistant Haemonchus contortus has been examined. For all BZs, the binding was substantially lower in the resistant isolate. The extent of ...

journal_title：Molecular and biochemical parasitology

authors： Lacey E,Prichard RK

更新日期：1986-05-01 00:00:00

abstract：:Telomeric DNA of trypanosomatids possesses a modified thymine base, called base J, that is synthesized in a two-step process; the base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU) and a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). To examine the importa...

journal_title：Molecular and biochemical parasitology

authors： Bullard W,Cliffe L,Wang P,Wang Y,Sabatini R

更新日期：2015-12-01 00:00:00

abstract：:The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

journal_title：Molecular and biochemical parasitology

authors： DaRocha WD,Otsu K,Teixeira SM,Donelson JE

更新日期：2004-02-01 00:00:00

abstract：:The promoter region of the ribosomal RNA (rRNA) genes of Leishmania amazonensis was characterised and the transcription start point, defined by primer extension, was shown to be a T residue, 1048 nucleotides upstream of the beginning of the 18S sequence. A repetitive element of 60 bp was identified in the intergenic s...

journal_title：Molecular and biochemical parasitology

authors： Uliana SR,Fischer W,Stempliuk VA,Floeter-Winter LM

更新日期：1996-02-01 00:00:00

abstract：:A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

journal_title：Molecular and biochemical parasitology

authors： Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

更新日期：2012-09-01 00:00:00

abstract：:Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughte...

journal_title：Molecular and biochemical parasitology

authors： Hines JC,Engel ML,Zhao H,Ray DS

更新日期：2001-06-01 00:00:00

abstract：:Trypanosoma brucei Tim17(TbTim17), the single member of the Tim17/23/22 protein family, is an essential component of the translocase of the mitochondrial inner membrane (TIM). In spite of the conserved secondary structure, the primary sequence of TbTim17, particularly the N-terminal hydrophilic region, is significantl...

journal_title：Molecular and biochemical parasitology

authors： Weems E,Singha UK,Smith JT,Chaudhuri M

更新日期：2017-12-01 00:00:00

abstract：:Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

journal_title：Molecular and biochemical parasitology

authors： Phillips MA,Wang CC

更新日期：1987-01-02 00:00:00

abstract：:Human hookworms (Ancylostoma duodenale, Necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. Mass drug administration (MDA) with benzimidazole (BZ) anthelmintics has the potential to greatly reduce morbidity...

journal_title：Molecular and biochemical parasitology

authors： Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

更新日期：2007-12-01 00:00:00

abstract：:The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

journal_title：Molecular and biochemical parasitology

authors： Cameron S,Fyffe SA,Goldie S,Hunter WN

更新日期：2008-04-01 00:00:00

abstract：:The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity...

journal_title：Molecular and biochemical parasitology

authors： Watkins WM,Sixsmith DG,Chulay JD,Spencer HC

更新日期：1985-01-01 00:00:00

abstract：:Trypanosoma cruzi, the aetiological agent of Chagas disease, can obtain L-glutamine (Gln) through the enzyme glutamine synthetase (GS) using glutamate (Glu) and ammonia as substrates. In this work, we show additional non-canonical roles for this amino acid: its involvement in ATP maintenance and parasite survival unde...

journal_title：Molecular and biochemical parasitology

authors： Damasceno FS,Barisón MJ,Crispim M,Souza ROO,Marchese L,Silber AM

更新日期：2018-09-01 00:00:00

abstract：:A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

journal_title：Molecular and biochemical parasitology

authors： Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

更新日期：2004-03-01 00:00:00

abstract：:The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

journal_title：Molecular and biochemical parasitology

authors： Price HP,Stark M,Smith B,Smith DF

更新日期：2007-10-01 00:00:00

abstract：:The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

journal_title：Molecular and biochemical parasitology

authors： Coppel RL

更新日期：1992-02-01 00:00:00

abstract：:An NADH-cytochrome c reductase (complex I-III) was isolated from Ascaris suum muscle mitochondria. The enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees C with NADH but not with NADPH, and retained its sensitivity to rotenone, piericidin A and 2-heptyl-4-hydroxyquin...

journal_title：Molecular and biochemical parasitology

authors： Takamiya S,Furushima R,Oya H

更新日期：1984-10-01 00:00:00

abstract：:Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recent...

journal_title：Molecular and biochemical parasitology

authors： Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

更新日期：1999-03-15 00:00:00

abstract：:Strongyloides and related genera are advantageous subjects for transgenesis in parasitic nematodes, primarily by gonadal microinjection as has been used with Caenorhabditis elegans. Transgenesis has been achieved in Strongyloides stercoralis and in Parastrongyloides trichosuri, but both of these lack well-adapted, con...

journal_title：Molecular and biochemical parasitology

authors： Li X,Shao H,Junio A,Nolan TJ,Massey HC Jr,Pearce EJ,Viney ME,Lok JB

更新日期：2011-10-01 00:00:00

abstract：:The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation ...

journal_title：Molecular and biochemical parasitology

authors： Ramos F,García G,Valadez A,Morán P,González E,Gómez A,Melendro EI,Valenzuela O,Ximénez C

更新日期：2005-06-01 00:00:00

abstract：:A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

journal_title：Molecular and biochemical parasitology

authors： Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

更新日期：2003-09-01 00:00:00

abstract：:A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

journal_title：Molecular and biochemical parasitology

authors： Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

更新日期：1995-02-01 00:00:00

abstract：:Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fa...

journal_title：Molecular and biochemical parasitology

authors： Dame JB,Yowell CA,Courtney CH,Lindgren WG

更新日期：1991-04-01 00:00:00