Genomic organisation of nuclear tRNAGly and tRNALeu genes in Trypanosoma brucei.

abstract：:Glycosomes are peroxisome-like organelles present in trypanosomatid pathogens. These organelles compartmentalize glycolysis, among other reactions, and are essential in both bloodstream and procyclic form Trypanosoma brucei. Peroxins (PEXs) are proteins necessary for biogenesis of peroxisomes and glycosomes. In each a...

journal_title：Molecular and biochemical parasitology

authors： Banerjee SK,Kessler PS,Saveria T,Parsons M

更新日期：2005-07-01 00:00:00

abstract：:Schistosomiasis remains a severe problem of public health in developing countries. Several reports show that praziquantel, the drug of choice for treating schistosomiasis, can select Schistosoma mansoni strains resistant to the drug. Thus, developing new drugs against this parasitosis is a highly desirable goal. Curcu...

journal_title：Molecular and biochemical parasitology

authors： Morais ER,Oliveira KC,Magalhães LG,Moreira EB,Verjovski-Almeida S,Rodrigues V

更新日期：2013-02-01 00:00:00

abstract：:Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable me...

journal_title：Molecular and biochemical parasitology

authors： Rofatto HK,Tararam CA,Borges WC,Wilson RA,Leite LC,Farias LP

更新日期：2009-07-01 00:00:00

abstract：:The apicomplexa parasite Toxoplasma gondii expresses two distinct proliferating cell nuclear antigens (PCNA) that exhibit distinct patterns of subcellular localization during tachyzoite growth. In all cell cycle phases, TgPCNA1 is concentrated in the nucleus, while TgPCNA2 is only concentrated in the nucleus during S-...

journal_title：Molecular and biochemical parasitology

authors： Guerini MN,Behnke MS,White MW

更新日期：2005-07-01 00:00:00

abstract：:In Leishmania mexicana two genes were detected coding for different isoforms of the glycolytic enzyme phosphoglycerate kinase. This situation contrasts with that observed in other Trypanosomatidae (Trypanosoma brucei, Trypanosoma congolense, Crithidia fasciculata) analyzed previously, which all contain three different...

journal_title：Molecular and biochemical parasitology

authors： Adjé CA,Opperdoes FR,Michels PA

更新日期：1997-12-01 00:00:00

abstract：:Trophozoites of Entamoeba invadens were able to ingest glucopolysaccharides and metabolize them. An activity capable of degrading these substrates was purified to apparent homogeneity. The enzyme was identified as beta-amylase (alpha-1,4-D-glucan maltohydrolase EC 3.2.1.2): It was active against glycogen, amylose and ...

journal_title：Molecular and biochemical parasitology

authors： Serafimova F,Franz A,Werries E

更新日期：1996-12-20 00:00:00

abstract：:A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

journal_title：Molecular and biochemical parasitology

authors： el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

更新日期：1991-11-01 00:00:00

abstract：:Precipitin arcs formed after immunoelectrophoresis of Schistosoma mansoni egg, worm and cercarial antigens with polyspecific rabbit antisera have been 'stained' at neutral pH with chromogenic enzyme substrates specific for proteases and peptidases. A total of 7 antigenically distinct enzymes with peptidolytic properti...

journal_title：Molecular and biochemical parasitology

authors： Doenhoff MJ,Modha J,Curtis RH,Adeoye GO

更新日期：1988-12-01 00:00:00

abstract：:Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was construct...

journal_title：Molecular and biochemical parasitology

authors： Sá-Carvalho D,Traub-Cseko YM

更新日期：1995-06-01 00:00:00

abstract：:Tropomyosins are a family of actin-binding proteins with diverse roles in actin filament function. One of the best characterized roles is the regulation of muscle contraction. Tropomyosin isoforms can be generated from different genes, and from alternative promoters and alternative splicing from the same gene. In this...

journal_title：Molecular and biochemical parasitology

authors： Koziol U,Costábile A,Domínguez MF,Iriarte A,Alvite G,Kun A,Castillo E

更新日期：2011-02-01 00:00:00

abstract：:While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of d...

journal_title：Molecular and biochemical parasitology

authors： Bethke LL,Zilversmit M,Nielsen K,Daily J,Volkman SK,Ndiaye D,Lozovsky ER,Hartl DL,Wirth DF

更新日期：2006-11-01 00:00:00

abstract：:The aspartic protease cathepsin D (Clan AA, Family A1) is expressed in the schistosome gut where it plays an apical role in the digestion of hemoglobin released from ingested erythrocytes. In this report, RNA interference approaches were employed to investigate the effects of knockdown of schistosome cathepsin D. Cult...

journal_title：Molecular and biochemical parasitology

authors： Morales ME,Rinaldi G,Gobert GN,Kines KJ,Tort JF,Brindley PJ

更新日期：2008-02-01 00:00:00

abstract：:Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lyso...

journal_title：Molecular and biochemical parasitology

authors： Vanácová S,Tachezy J,Ullu E,Tschudi C

更新日期：2001-07-01 00:00:00

abstract：:The emergence of drug-resistant Leishmania species is a significant problem in several countries. A comparative proteomic analysis of antimony-susceptible and antimony-resistant Leishmania braziliensis (LbSbR) and Leishmania infantum chagasi (LcSbR) lines was carried out using two-dimensional gel electrophoresis (2-DE...

journal_title：Molecular and biochemical parasitology

authors： Matrangolo FS,Liarte DB,Andrade LC,de Melo MF,Andrade JM,Ferreira RF,Santiago AS,Pirovani CP,Silva-Pereira RA,Murta SM

更新日期：2013-08-01 00:00:00

abstract：:Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

journal_title：Molecular and biochemical parasitology

authors： Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

更新日期：2010-11-01 00:00:00

abstract：:An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC 2.4.2.1). ANP is specific for adenine nucleosides...

journal_title：Molecular and biochemical parasitology

authors： Savarese TM,El Kouni MH

更新日期：2014-03-01 00:00:00

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00

abstract：:In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

journal_title：Molecular and biochemical parasitology

authors： Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

更新日期：1991-06-01 00:00:00

abstract：:A Fasciola hepatica cDNA clone of 1752 bp was isolated from an adult worm cDNA expression library by immunological screening using a rabbit serum against the excretory-secretory antigens. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 489 codons which encoded a 55 kDa polypeptide...

journal_title：Molecular and biochemical parasitology

authors： Salazar-Calderón M,Martín-Alonso JM,Castro AM,Parra F

更新日期：2003-01-01 00:00:00

abstract：:Genetic transformation of malaria parasites has been limited by the number of selectable markers available. For the rodent malaria parasite, Plasmodium berghei, only a single selection marker has been at hand, utilising the dihydrofolate reductase-thymidylate synthase gene from either P. berghei or Toxoplasma gondii t...

journal_title：Molecular and biochemical parasitology

authors： de Koning-Ward TF,Fidock DA,Thathy V,Menard R,van Spaendonk RM,Waters AP,Janse CJ

更新日期：2000-03-05 00:00:00

abstract：:The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

journal_title：Molecular and biochemical parasitology

authors： Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

更新日期：2001-08-01 00:00:00

abstract：:Recent investigations into quinoline and phenanthrene methanol resistance in Plasmodium falciparum have described a linkage between amplification of the mdr homologue pfmdr1 and decreased susceptibility to mefloquine (MQ) and halofantrine (HF). We have examined the current theories on cross-resistance patterns and pfm...

journal_title：Molecular and biochemical parasitology

authors： Ritchie GY,Mungthin M,Green JE,Bray PG,Hawley SR,Ward SA

更新日期：1996-12-02 00:00:00

abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

journal_title：Molecular and biochemical parasitology

authors： Dissanayake S,Xu M,Piessens WF

更新日期：1992-12-01 00:00:00

abstract：:Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2 beta genes have been isolated and sequenced. Three of them had a single TcP2 beta gene, while the 3.8-kb-long DNA segment encoding the TcP2 beta-H1.8 locus showed two TcP2 beta genes arranged in tandem. These genes were physically connected by a 428-...

journal_title：Molecular and biochemical parasitology

authors： Vazquez MP,Schijman AG,Levin MJ

更新日期：1994-04-01 00:00:00

abstract：:In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

journal_title：Molecular and biochemical parasitology

authors： Navarro M,Cross GA

更新日期：1998-07-01 00:00:00

abstract：:One of the candidate schistosome antigens for the development of a circulating antigen detection diagnostic assay is the circulating cathodic antigen (CCA). Detection of CCA in urine provides a non-invasive assay with high sensitivity. Previously we reported that CCA is secreted in patients' urine as a small molecular...

journal_title：Molecular and biochemical parasitology

authors： Abdeen HH,Attallah AF,Mansour MM,Harrison RA

更新日期：1999-06-25 00:00:00

abstract：:Studies were made of the effects of diamphenethide-amine on glucose transport, glycogen breakdown, adenine nucleotides, metabolites and excretory products in both parenchymal and bile duct flukes in vitro and in bile duct flukes in vivo. The most consistent and pronounced effect observed was an elevation of malate lev...

journal_title：Molecular and biochemical parasitology

authors： Edwards SR,Campbell AJ,Sheers M,Moore RJ,Montague PE

更新日期：1981-04-01 00:00:00

abstract：:Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the i...

journal_title：Molecular and biochemical parasitology

authors： Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS

更新日期：2013-10-01 00:00:00

abstract：:We previously customized a Nipkow spinning disk confocal microscope (SDCM) to acquire 4D data for live, intraerythrocytic malarial parasites [Gligorijevic B, McAllister R, Urbach JS, Roepe, PD. Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 1. Quantification of hemozoin development fo...

journal_title：Molecular and biochemical parasitology

authors： Gligorijevic B,Purdy K,Elliott DA,Cooper RA,Roepe PD

更新日期：2008-05-01 00:00:00

abstract：:Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

journal_title：Molecular and biochemical parasitology

authors： Cacciò SM,Ryan U

更新日期：2008-08-01 00:00:00