Accurate transcription of a cloned gene from Babesia bovis in Saccharomyces cerevisiae.

abstract：:The variant-specific surface glycoprotein (VSG) genes of Trypanosoma brucei are invariably expressed near the ends of chromosomes (telomeres). We have targeted a VSG gene expression site (ES) promoter driving a selectable marker gene (neomycin phosphotransferase) into a chromosome-internal transcription unit, the tubu...

journal_title：Molecular and biochemical parasitology

authors： Zomerdijk JC,Kieft R,Borst P

更新日期：1993-02-01 00:00:00

abstract：:In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

journal_title：Molecular and biochemical parasitology

authors： Navarro M,Cross GA

更新日期：1998-07-01 00:00:00

abstract：:We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

journal_title：Molecular and biochemical parasitology

authors： Gilchrist CA,Streets HL,Ackers JP,Hall R

更新日期：1995-10-01 00:00:00

abstract：:Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. T...

journal_title：Molecular and biochemical parasitology

authors： Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

更新日期：1988-02-01 00:00:00

abstract：:Erythrocyte invasion by malaria parasites is a multi-step process requiring specific molecular interactions between merozoites and erythrocyte surface receptors. Human Duffy blood group protein is the receptor for Plasmodium vivax merozoite invasion to red blood cells. The cognate parasite ligand for Duffy protein is ...

journal_title：Molecular and biochemical parasitology

authors： Xu L,Niu J,Chaudhuri A

更新日期：2006-04-01 00:00:00

abstract：:Hamsters infected with Opisthorchis viverrini, a liver fluke of man, showed an increased deposition of collagen in their livers. However, increased collagen breakdown as well as its synthesis were observed in the infected livers. Thus, stimulated synthesis might be the main factor responsible for the net increase in c...

journal_title：Molecular and biochemical parasitology

authors： Hutadilok N,Ruenwongsa P

更新日期：1983-05-01 00:00:00

abstract：:Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoites store TLN4 in the micronemes and secrete it in response to elevated calcium, ...

journal_title：Molecular and biochemical parasitology

authors： Laliberté J,Carruthers VB

更新日期：2011-05-01 00:00:00

abstract：:The effect of 9 monosaccharides which constitute cell surface carbohydrates on the infection of bovine embryonic skin and muscle (BESM) cells by Trypanosoma cruzi trypomastigotes and Toxoplasma gondii tachyzoites was assayed. Most of the monosaccharides tested stimulated the infection of BESM cells by T. gondii; none ...

journal_title：Molecular and biochemical parasitology

authors： Crane MS,Dvorak JA

更新日期：1982-05-01 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00

abstract：:Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughte...

journal_title：Molecular and biochemical parasitology

authors： Hines JC,Engel ML,Zhao H,Ray DS

更新日期：2001-06-01 00:00:00

abstract：:The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates ...

journal_title：Molecular and biochemical parasitology

authors： Marché S,Roth C,Philippe H,Dollet M,Baltz T

更新日期：1995-04-01 00:00:00

abstract：:We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

journal_title：Molecular and biochemical parasitology

authors： Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

更新日期：1992-09-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00

abstract：:Giardia lamblia depends on glycolysis to obtain ATP, highlighting the suitability of glycolytic enzymes as targets for drug design. We studied triosephosphate isomerase from G. lamblia (GlTIM) as a potential species-specific drug target. Cysteine-reactive agents were used as probes, in order to test those regions near...

journal_title：Molecular and biochemical parasitology

authors： Enriquez-Flores S,Rodriguez-Romero A,Hernandez-Alcantara G,De la Mora-De la Mora I,Gutierrez-Castrellon P,Carvajal K,Lopez-Velazquez G,Reyes-Vivas H

更新日期：2008-02-01 00:00:00

abstract：:Clones which were selected from a Toxoplasma gondii expression library with the immune serum from a T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and...

journal_title：Molecular and biochemical parasitology

authors： Mevelec MN,Chardès T,Mercereau-Puijalon O,Bourguin I,Achbarou A,Dubremetz JF,Bout D

更新日期：1992-12-01 00:00:00

abstract：:Kinetoplast DNA (kDNA) was isolated from 56 stocks of Trypanosoma cruzi isolated from human patients, animals and insects from Brazil, Venezuela, Colombia and Costa Rica. Comparison of the patterns of digested kDNA on acrylamide gels led to the grouping of several stocks into two schizodemes. Schizodeme analysis was a...

journal_title：Molecular and biochemical parasitology

authors： Avila H,Goncalves AM,Nehme NS,Morel CM,Simpson L

更新日期：1990-09-01 00:00:00

abstract：:To further analyze the organization of the nuclear rDNA locus in Trypanosoma cruzi, genomic recombinant plasmid clones were constructed and isolated after hybridization with rRNA molecules as hybridization probes. Approximately 11 kilobase pairs from the cistron were cloned in three recombinant plasmids carrying adjac...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Díaz-de Léon F,Castañeda M

更新日期：1988-01-15 00:00:00

abstract：:Distinct 3'- and 5'-nucleotidase activities were localized to the surface membrane of Leishmania donovani promastigotes using enzymatic reactions with live intact cells and fine structure enzyme-mediated cytochemical reactions with intact cells, cell homogenates, and isolated surface membranes. The results indicated t...

journal_title：Molecular and biochemical parasitology

authors： Dwyer DM,Gottlieb M

更新日期：1984-02-01 00:00:00

abstract：:Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatogra...

journal_title：Molecular and biochemical parasitology

authors： Liebau E,Wildenburg G,Brophy PM,Walter RD,Henkle-Dührsen K

更新日期：1996-09-01 00:00:00

abstract：:The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

journal_title：Molecular and biochemical parasitology

authors： Chen M,Li E,Stanley SL Jr

更新日期：2004-10-01 00:00:00

abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

journal_title：Molecular and biochemical parasitology

authors： Dissanayake S,Xu M,Piessens WF

更新日期：1992-12-01 00:00:00

abstract：:The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prol...

journal_title：Molecular and biochemical parasitology

authors： Adamson R,Logan M,Kinnaird J,Langsley G,Hall R

更新日期：2000-02-25 00:00:00

abstract：:We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits allelic variation. One lo...

journal_title：Molecular and biochemical parasitology

authors： Rangarajan D,Harvey TI,Barry JD

更新日期：2000-02-05 00:00:00

abstract：:Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A...

journal_title：Molecular and biochemical parasitology

authors： Arbabi M,Hooshyar H,Lotfinia M,Bakhshi MA

更新日期：2020-03-01 00:00:00

abstract：:The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host i...

journal_title：Molecular and biochemical parasitology

authors： Aragon AD,Imani RA,Blackburn VR,Cunningham C

更新日期：2008-12-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence anal...

journal_title：Molecular and biochemical parasitology

authors： Barnes DA,Bonnin A,Huang JX,Gousset L,Wu J,Gut J,Doyle P,Dubremetz JF,Ward H,Petersen C

更新日期：1998-10-30 00:00:00

abstract：:Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

journal_title：Molecular and biochemical parasitology

authors： Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

更新日期：1998-08-01 00:00:00

abstract：:Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis in humans and animals. To invade host cells, T. gondii deploys the contents of its apically oriented secretory organelles that include the micronemes. Contained within the micronemes are proteins that possess adhesive motifs resembling t...

journal_title：Molecular and biochemical parasitology

authors： Harper JM,Hoff EF,Carruthers VB

更新日期：2004-04-01 00:00:00

abstract：:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measur...

journal_title：Molecular and biochemical parasitology

authors： Walsh MJ,Blum JJ

更新日期：1992-02-01 00:00:00

abstract：:Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

journal_title：Molecular and biochemical parasitology

authors： Campos MC,Leon LL,Taylor MC,Kelly JM

更新日期：2014-01-01 00:00:00