Abstract:
:Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughter molecules with a single nick or gap in the L strand at either ori A or ori B. Some of the gapped molecules contain ribonucleotides at the 5' side of the gap. We have investigated the ability of recombinant forms of kinetoplast replication proteins, DNA polymerase beta and structure specific endonuclease 1, to repair gaps in a model minicircle substrate. Structure specific endonuclease 1 was shown to efficiently remove all ribonucleotides from the 5' side of the model substrate by stepwise cleavage of the RNA primer. Polymerase beta was then able to extend the 3' terminus of the gap to yield a nicked molecule capable of covalent joining by a DNA ligase. These results demonstrate that the nuclease and polymerase enzymes present at antipodal protein complexes flanking the kinetoplast disk are capable of complete RNA primer removal and subsequent gap filling of newly synthesized minicircle L strands.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Hines JC,Engel ML,Zhao H,Ray DSdoi
10.1016/s0166-6851(01)00272-9subject
Has Abstractpub_date
2001-06-01 00:00:00pages
63-7issue
1eissn
0166-6851issn
1872-9428pii
S0166-6851(01)00272-9journal_volume
115pub_type
杂志文章abstract::A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into tw...
journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
doi:10.1016/j.molbiopara.2006.07.005
更新日期:2006-12-01 00:00:00
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章,评审
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更新日期:2008-08-01 00:00:00
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
doi:10.1016/0166-6851(94)90058-2
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journal_title:Molecular and biochemical parasitology
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更新日期:2012-06-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00