RNA primer removal and gap filling on a model minicircle replication intermediate.


:Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughter molecules with a single nick or gap in the L strand at either ori A or ori B. Some of the gapped molecules contain ribonucleotides at the 5' side of the gap. We have investigated the ability of recombinant forms of kinetoplast replication proteins, DNA polymerase beta and structure specific endonuclease 1, to repair gaps in a model minicircle substrate. Structure specific endonuclease 1 was shown to efficiently remove all ribonucleotides from the 5' side of the model substrate by stepwise cleavage of the RNA primer. Polymerase beta was then able to extend the 3' terminus of the gap to yield a nicked molecule capable of covalent joining by a DNA ligase. These results demonstrate that the nuclease and polymerase enzymes present at antipodal protein complexes flanking the kinetoplast disk are capable of complete RNA primer removal and subsequent gap filling of newly synthesized minicircle L strands.


Mol Biochem Parasitol


Hines JC,Engel ML,Zhao H,Ray DS




Has Abstract


2001-06-01 00:00:00














  • Actin sequestering protein, profilin, regulates intracellular vesicle transport in Leishmania.

    abstract::Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

    更新日期:2020-07-01 00:00:00

  • Dominant lethality and insect population control.

    abstract::Recent advances in insect genetic engineering have opened up new possibilities in the genetic control of insect vectors of human diseases. We outline the current state of the sterile insect technique and show how the use of engineered dominant lethals can greatly increase the effectiveness of this approach. We conside...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Alphey L,Andreasen M

    更新日期:2002-05-01 00:00:00

  • Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes.

    abstract::The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pouvelle B,Gormley JA,Taraschi TF

    更新日期:1994-07-01 00:00:00

  • The T687G SNP in a P-glycoprotein gene of Fasciola hepatica is not associated with resistance to triclabendazole in two resistant Australian populations.

    abstract::Triclabendazole (TCBZ) is widely used for control of Fasciola hepatica (liver fluke) in animals and humans and resistance to this drug is now widespread. However, the mechanism of resistance to TCBZ is not known. A T687G single nucleotide polymorphism (SNP) in a P-glycoprotein gene was proposed as a molecular marker f...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Elliott TP,Spithill TW

    更新日期:2014-11-01 00:00:00

  • Trichomonas vaginalis ribosomal RNA: identification and characterisation of the transcription promoter and terminator sequences.

    abstract::Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Franco B,Hernández R,López-Villaseñor I

    更新日期:2012-09-01 00:00:00

  • The interaction of artemisinin with malarial hemozoin.

    abstract::[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hong YL,Yang YZ,Meshnick SR

    更新日期:1994-01-01 00:00:00

  • Expression of a stage-specific lipophosphoglycan in Leishmania major amastigotes.

    abstract::Amastigotes of Leishmania major were isolated from infected mice and radiolabeled for 2 h with [3H]galactose. An acidic [3H]glycoconjugate was extracted from a dilipidated residue fraction with the solvent water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017). The radioactivity labeled glycoconjugate was found t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Turco SJ,Sacks DL

    更新日期:1991-03-01 00:00:00

  • A kinetoplast DNA probe diagnostic for Leishmania major: sequence homologies between regions of Leishmania minicircles.

    abstract::A restriction fragment from a cloned kinetoplast minicircle DNA has been shown to be diagnostic for Leishmania major. This 402-bp TaqI fragment has been used routinely (as a radiolabelled probe) to detect 10(4) parasites in simple dot blots, both experimentally and in epidemiological surveys. It positively identified ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Smith DF,Searle S,Ready PD,Gramiccia M,Ben-Ismail R

    更新日期:1989-12-01 00:00:00

  • Transgenic Plasmodium berghei sporozoites expressing beta-galactosidase for quantification of sporozoite transmission.

    abstract::Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Engelmann S,Sinnis P,Matuschewski K

    更新日期:2006-03-01 00:00:00

  • Stable transfection and continuous expression of heterologous genes in Entamoeba invadens.

    abstract::Amoebiasis is spread by the ingestion of dormant Entamoeba histolytica cysts. Intervention of encystation could break the transmission cycle, thereby reducing disease burden. The model system used to study trophozoite to cyst differentiation is Entamoeba invadens. Here we describe an electroporation-based method for s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singh N,Ojha S,Bhattacharya A,Bhattacharya S

    更新日期:2012-07-01 00:00:00

  • A schizont-derived protein, TpSCOP, is involved in the activation of NF-kappaB in Theileria parva-infected lymphocytes.

    abstract::Theileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the nuclear factor-kappa B (NF-kappa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hayashida K,Hattori M,Nakao R,Tanaka Y,Kim JY,Inoue N,Nene V,Sugimoto C

    更新日期:2010-11-01 00:00:00

  • Identification of Entamoeba histolytica thiol-specific antioxidant as a GalNAc lectin-associated protein.

    abstract::Entamoeba histolytica is a human intestinal parasite that causes amebic dysentery. A cell surface amebic adhesin, the galactose and N-acetyl-D-galactosamine inhibitable (GalNAc) lectin mediates amebic adherence to and contact-dependent killing of host cells. Previous work has suggested that the GalNAc lectin transduce...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hughes MA,Lee CW,Holm CF,Ghosh S,Mills A,Lockhart LA,Reed SL,Mann BJ

    更新日期:2003-04-03 00:00:00

  • Kinetoplast DNA analysis of four Trypanosoma evansi strains.

    abstract::Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomes, is a network of thousands of topologically interlocked DNA minicircles and about 50 maxicircles. In this study, we have analysed the kDNA molecules of 6 strains of Trypanosoma evansi from different geographical areas. 2 strains were found to be dyskinetopl...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ou YC,Giroud C,Baltz T

    更新日期:1991-05-01 00:00:00

  • Recent independent evolution of msp1 polymorphism in Plasmodium vivax and related simian malaria parasites.

    abstract::The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

    更新日期:2007-11-01 00:00:00

  • TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei.

    abstract::The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Price HP,Stark M,Smith B,Smith DF

    更新日期:2007-10-01 00:00:00

  • Cloning of a cDNA encoding the dense granule protein GRA3 from Toxoplasma gondii.

    abstract::GRA3 is a 30-kDa protein located inside the dense granules of Toxoplasma gondii. Following invasion and exocytosis of dense granules within the parasitophorous vacuole, GRA3 becomes associated with the parasitophorous vacuolar membrane (PVM) and extensions of the PVM which protrude into the cytoplasm. A partial cDNA e...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bermudes D,Dubremetz JF,Achbarou A,Joiner KA

    更新日期:1994-12-01 00:00:00

  • Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity.

    abstract::Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Hewitson JP,Grainger JR,Maizels RM

    更新日期:2009-09-01 00:00:00

  • Unique kinetic mechanism of Plasmodium falciparum adenylosuccinate synthetase.

    abstract::Adenylosuccinate synthetase (AdSS) catalyses the Mg(2+) dependent formation of adenylosuccinate from IMP and aspartate, the reaction being driven by the hydrolysis of GTP to GDP. All characterized AdSS thus far exhibit a random kinetic mechanism. We present here kinetic evidence that unlike all other AdSS, Plasmodium ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Raman J,Mehrotra S,Anand RP,Balaram H

    更新日期:2004-11-01 00:00:00

  • Identification of Toxoplasma TgPH1, a pleckstrin homology domain-containing protein that binds to the phosphoinositide PI(3,5)P2.

    abstract::The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

    更新日期:2016-05-01 00:00:00

  • Studies on mitochondrial ATPase of Leishmania donovani using digitonin-permeabilized promastigotes.

    abstract::Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Das A

    更新日期:1993-08-01 00:00:00

  • Isolation of lambda amp3 genomic recombinants coding for antigens of Eimeria tenella.

    abstract::Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vacci...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Clarke LE,Messer LI,Greenwood NM,Wisher MH

    更新日期:1987-01-02 00:00:00

  • Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.

    abstract::A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liu X,Chang KP

    更新日期:1994-08-01 00:00:00

  • Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach.

    abstract::Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

    更新日期:2021-01-07 00:00:00

  • Purification and partial characterization of an unusual protein of Plasmodium falciparum: histidine-rich protein II.

    abstract::The human malarial parasite Plasmodium falciparum secretes a histidine-rich protein (HRP-II) from infected erythrocytes. HRP-II has a very high content of histidine (H) (34%), alanine (A) (37%) and aspartic acid (D) (10%) and many contiguous repeats of the sequences AHH and AHHAAD. The histidine content of the protein...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Panton LJ,McPhie P,Maloy WL,Wellems TE,Taylor DW,Howard RJ

    更新日期:1989-06-15 00:00:00

  • Molecular cloning of Trypanosoma brucei CK2 catalytic subunits: the alpha isoform is nucleolar and phosphorylates the nucleolar protein Nopp44/46.

    abstract::We have demonstrated previously that Nopp44/46, an abundant nucleolar phosphoprotein of Trypanosoma brucei, is associated with a protein kinase. In many organisms multiple nucleolar proteins are phosphorylated by the protein kinase CK2, formerly known as casein kinase II. Here we report the identification of two T. br...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Park JH,Brekken DL,Randall AC,Parsons M

    更新日期:2002-01-01 00:00:00

  • Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei.

    abstract::Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

    更新日期:2006-09-01 00:00:00

  • A population of tRNA-derived small RNAs is actively produced in Trypanosoma cruzi and recruited to specific cytoplasmic granules.

    abstract::Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/P...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Garcia-Silva MR,Frugier M,Tosar JP,Correa-Dominguez A,Ronalte-Alves L,Parodi-Talice A,Rovira C,Robello C,Goldenberg S,Cayota A

    更新日期:2010-06-01 00:00:00

  • Cryptosporidium parvum glycoprotein gp40 localizes to the sporozoite surface by association with gp15.

    abstract::Cryptosporidium spp. are waterborne apicomplexan parasites responsible for outbreaks of diarrheal disease worldwide. Antigens involved in zoite invasion into host cells have been the focus of many investigations as these may prove to be good vaccine candidates. gp40/15 is a zoite antigen synthesized as a precursor pro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: O'Connor RM,Wanyiri JW,Cevallos AM,Priest JW,Ward HD

    更新日期:2007-11-01 00:00:00

  • Effects of curcumin on the parasite Schistosoma mansoni: a transcriptomic approach.

    abstract::Schistosomiasis remains a severe problem of public health in developing countries. Several reports show that praziquantel, the drug of choice for treating schistosomiasis, can select Schistosoma mansoni strains resistant to the drug. Thus, developing new drugs against this parasitosis is a highly desirable goal. Curcu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Morais ER,Oliveira KC,Magalhães LG,Moreira EB,Verjovski-Almeida S,Rodrigues V

    更新日期:2013-02-01 00:00:00

  • Identification of a domain of Trypanosoma cruzi metacyclic trypomastigote surface molecule gp82 required for attachment and invasion of mammalian cells.

    abstract::Recombinant proteins and synthetic peptides representing various sequences of gp82, a surface glycoprotein of Trypanosoma cruzi metacyclic trypomastigotes implicated in mammalian cell invasion, were used in this study aiming at the identification of the domain(s) of this molecule required for interaction with target c...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Santori FR,Dorta ML,Juliano L,Juliano MA,da Silveira JF,Ruiz RC,Yoshida N

    更新日期:1996-06-01 00:00:00