RNA primer removal and gap filling on a model minicircle replication intermediate.

Abstract:

:Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughter molecules with a single nick or gap in the L strand at either ori A or ori B. Some of the gapped molecules contain ribonucleotides at the 5' side of the gap. We have investigated the ability of recombinant forms of kinetoplast replication proteins, DNA polymerase beta and structure specific endonuclease 1, to repair gaps in a model minicircle substrate. Structure specific endonuclease 1 was shown to efficiently remove all ribonucleotides from the 5' side of the model substrate by stepwise cleavage of the RNA primer. Polymerase beta was then able to extend the 3' terminus of the gap to yield a nicked molecule capable of covalent joining by a DNA ligase. These results demonstrate that the nuclease and polymerase enzymes present at antipodal protein complexes flanking the kinetoplast disk are capable of complete RNA primer removal and subsequent gap filling of newly synthesized minicircle L strands.

journal_name

Mol Biochem Parasitol

authors

Hines JC,Engel ML,Zhao H,Ray DS

doi

10.1016/s0166-6851(01)00272-9

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

63-7

issue

1

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(01)00272-9

journal_volume

115

pub_type

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