Arp1, an actin-related protein, in Plasmodium berghei.

abstract：:The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

journal_title：Molecular and biochemical parasitology

authors： DaRocha WD,Otsu K,Teixeira SM,Donelson JE

更新日期：2004-02-01 00:00:00

abstract：:Messenger RNA has been isolated from adults and eggs of Schistosoma mansoni and translated in vitro in mRNA dependent rabbit reticulocyte lysate. The in vitro translation products have been immunoprecipitated using a wide variety of hyperimmune and infection sera from rabbits, mice and humans. A large number of in vit...

journal_title：Molecular and biochemical parasitology

authors： Taylor DW,Cordingley JS,Butterworth AE

更新日期：1984-03-01 00:00:00

abstract：:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

journal_title：Molecular and biochemical parasitology

authors： Thompson SN,Platzer EG,Lee RW

更新日期：1987-01-02 00:00:00

abstract：:Plasmodium LCCL proteins comprise a family of six proteins that function as a protein complex and have essential roles in sporozoite transmission. In Plasmodium berghei, family members PbLAP1, PbLAP2 and PbLAP3 have been shown to be expressed in gametocytes and, following gametogenesis and fertilization, to be targete...

journal_title：Molecular and biochemical parasitology

authors： Saeed S,Carter V,Tremp AZ,Dessens JT

更新日期：2013-05-01 00:00:00

abstract：:Soon after infecting a mammalian host, cercariae of Schistosoma mansoni rapidly undergo a series of morphologic and biochemical adaptations associated with transformation to their next developmental stage, the schistosomulum. Few of these changes are associated with alterations in gene expression except for an apparen...

journal_title：Molecular and biochemical parasitology

authors： Blanton RE,Licate LS

更新日期：1992-04-01 00:00:00

abstract：:Hamsters infected with Opisthorchis viverrini, a liver fluke of man, showed an increased deposition of collagen in their livers. However, increased collagen breakdown as well as its synthesis were observed in the infected livers. Thus, stimulated synthesis might be the main factor responsible for the net increase in c...

journal_title：Molecular and biochemical parasitology

authors： Hutadilok N,Ruenwongsa P

更新日期：1983-05-01 00:00:00

abstract：:In silico analyses of Leishmania spp. genome data are a powerful resource to improve the understanding of these pathogens' biology. Trypanosomatids such as Leishmania spp. have their protein-coding genes grouped in long polycistronic units of functionally unrelated genes. The control of gene expression happens by a va...

journal_title：Molecular and biochemical parasitology

authors： Vasconcelos EJ,Terrão MC,Ruiz JC,Vêncio RZ,Cruz AK

更新日期：2012-06-01 00:00:00

abstract：:An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC 2.4.2.1). ANP is specific for adenine nucleosides...

journal_title：Molecular and biochemical parasitology

authors： Savarese TM,El Kouni MH

更新日期：2014-03-01 00:00:00

abstract：:The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions hav...

journal_title：Molecular and biochemical parasitology

authors： Sonda S,Fuchs N,Connolly B,Fernandez P,Gottstein B,Hemphill A

更新日期：1998-11-30 00:00:00

abstract：:A membrane-bound sialidase (EC 3.2.1.18) was found in procyclic trypomastigotes of Trypanosoma brucei. The mammalian stage bloodstream form, however, displayed no sialidase activity. This sialidase is an integral surface protein, linked to the membrane via a glycosylphosphatidylinositol anchor. After osmotic lysis and...

journal_title：Molecular and biochemical parasitology

authors： Engstler M,Reuter G,Schauer R

更新日期：1992-08-01 00:00:00

abstract：:Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our kn...

journal_title：Molecular and biochemical parasitology

authors： Faktorová D,Bär A,Hashimi H,McKenney K,Horák A,Schnaufer A,Rubio MAT,Alfonzo JD,Lukeš J

更新日期：2018-10-01 00:00:00

abstract：:Release of sporozoites from Eimeria oocysts/sporocysts is an essential step in the intracellular development of the parasite in its host. Little is known about this process except that elevated temperature (∼ 40 °C) plus trypsin and bile salts are required for sporozoite to escape from sporocysts. In this study, it wa...

journal_title：Molecular and biochemical parasitology

authors： Jenkins MC,O'Brien CN,Parker C

更新日期：2019-10-01 00:00:00

abstract：:The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

journal_title：Molecular and biochemical parasitology

authors： Barral V,This P,Imbert-Establet D,Combes C,Delseny M

更新日期：1993-06-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

journal_title：Molecular and biochemical parasitology

authors： Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

更新日期：1994-09-01 00:00:00

abstract：:Electron paramagnetic resonance spectrometry (EPR) techniques revealed a complex set of redox components in intact respiring cells of Tritrichomonas foetus, an aerotolerant anaerobic protozoon which lacks mitochondria. One of the two main systems of terminal respiration of this organism is the hydrogenosomal system, w...

journal_title：Molecular and biochemical parasitology

authors： Ohnishi T,Lloyd D,Lindmark DG,Müller M

更新日期：1980-10-01 00:00:00

abstract：:It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

journal_title：Molecular and biochemical parasitology

authors： Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

更新日期：1997-09-01 00:00:00

abstract：:A method for a 50-60-fold purification of a cysteine proteinase from trophozoites of Entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on Sephadex G-75, and preparative isoelectric focusing is described. The enzyme was examined for its proteolytic potencies towards native enzyme su...

journal_title：Molecular and biochemical parasitology

authors： Scholze H,Werries E

更新日期：1986-01-01 00:00:00

abstract：:Polyacrylamide gel electrophoresis of the total RNA extracted from cultures of Plasmodium falciparum resolved two major RNA components with estimated molecular weights of 1.3 X 10(6) and 0.72 - 0.74 X 10(6). Oligo(dT)-cellulose column chromatography of the total cellular RNA indicates that more than 90% of the total r...

journal_title：Molecular and biochemical parasitology

authors： Vezza AC,Trager W

更新日期：1981-12-01 00:00:00

abstract：:Antibodies were affinity purified from crude antiserum by elution from the 24 kDa region of preparative one-dimensional Western blots containing immobilized adult Schistosoma mansoni inner bilayer membrane proteins. They were shown to be specific for a single acidic polypeptide complex, Smgp24, following immunoblottin...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Barnard BJ,Podesta RB

更新日期：1988-11-01 00:00:00

abstract：:The apicomplexa parasite Toxoplasma gondii expresses two distinct proliferating cell nuclear antigens (PCNA) that exhibit distinct patterns of subcellular localization during tachyzoite growth. In all cell cycle phases, TgPCNA1 is concentrated in the nucleus, while TgPCNA2 is only concentrated in the nucleus during S-...

journal_title：Molecular and biochemical parasitology

authors： Guerini MN,Behnke MS,White MW

更新日期：2005-07-01 00:00:00

abstract：:We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

journal_title：Molecular and biochemical parasitology

authors： Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

更新日期：1992-09-01 00:00:00

abstract：:To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Van Roy J

更新日期：1982-09-01 00:00:00

abstract：:Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

journal_title：Molecular and biochemical parasitology

authors： Rayner JC,Huber CS,Galinski MR,Barnwell JW

更新日期：2004-02-01 00:00:00

abstract：:Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

journal_title：Molecular and biochemical parasitology

authors： Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

更新日期：1991-07-01 00:00:00

abstract：:Schistosomes have a complex life cycle (vertebrate and molluscan hosts as well as larvae living freely in water) in which they are exposed to different environments and temperatures (20 degrees C - 37 degrees C). Since heat shock genes are activated in response to stress and during development [1], it is of interest t...

journal_title：Molecular and biochemical parasitology

authors： Neumann S,Ziv E,Lantner F,Schechter I

更新日期：1992-12-01 00:00:00

abstract：:Cryptosporidium spp. are waterborne apicomplexan parasites responsible for outbreaks of diarrheal disease worldwide. Antigens involved in zoite invasion into host cells have been the focus of many investigations as these may prove to be good vaccine candidates. gp40/15 is a zoite antigen synthesized as a precursor pro...

journal_title：Molecular and biochemical parasitology

authors： O'Connor RM,Wanyiri JW,Cevallos AM,Priest JW,Ward HD

更新日期：2007-11-01 00:00:00

abstract：:The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

journal_title：Molecular and biochemical parasitology

authors： Pouvelle B,Gormley JA,Taraschi TF

更新日期：1994-07-01 00:00:00

abstract：:We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

journal_title：Molecular and biochemical parasitology

authors： Toye P,Remold H

更新日期：1989-06-01 00:00:00

abstract：:In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

journal_title：Molecular and biochemical parasitology

authors： Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

更新日期：1991-06-01 00:00:00

abstract：:Several proteins synthesized by mature asexual stages of Plasmodium falciparum interact with the erythrocyte membrane skeleton. One of these is the mature-parasite-infected erythrocyte surface antigen (MESA; also called PfEMP2), a phosphoprotein of 250-300 kDa, which is found on the internal face of the erythrocyte me...

journal_title：Molecular and biochemical parasitology

authors： Lustigman S,Anders RF,Brown GV,Coppel RL

更新日期：1990-01-15 00:00:00