Cysteine proteinase of Entamoeba histolytica. I. Partial purification and action on different enzymes.


:A method for a 50-60-fold purification of a cysteine proteinase from trophozoites of Entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on Sephadex G-75, and preparative isoelectric focusing is described. The enzyme was examined for its proteolytic potencies towards native enzyme substrates. The amebic proteinase directly inactivates aldolase and glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle as well as glucose-6-phosphate dehydrogenase from yeast. The inactivation of citrate synthase from porcine heart proceeds rather slowly, whereas malate dehydrogenase from porcine heart is not affected by the amebic proteinase under the condition used. With the exception of aldolase all inactivated enzyme substrates have been cleaved by limited proteolyses yielding major cleavage products. The inactivation of aldolase probably functions by the release of a small segment from a terminus being essential for aldolase activity.


Mol Biochem Parasitol


Scholze H,Werries E




Has Abstract


1986-01-01 00:00:00














  • RNA interference in Pratylenchus coffeae: knock down of Pc-pat-10 and Pc-unc-87 impedes migration.

    abstract::Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Joseph S,Gheysen G,Subramaniam K

    更新日期:2012-11-01 00:00:00

  • Design of Onchocerca DNA probes based upon analysis of a repeated sequence family.

    abstract::Repeated DNA sequences have been instrumental in the development of DNA probes for many different parasites. Isolation of such DNA probes has generally been accomplished by differential screening of genomic libraries with total genomic DNA preparations. In the current work, a rational design strategy is presented for ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zimmerman PA,Toe L,Unnasch TR

    更新日期:1993-04-01 00:00:00

  • Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense.

    abstract::The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

    更新日期:1995-02-01 00:00:00

  • Filarial parasites contain a ras homolog of the TC4/ran/Spil family.

    abstract::We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dissanayake S,Xu M,Piessens WF

    更新日期:1992-12-01 00:00:00

  • Ecdysteroids in adults of the nematode, Dirofilaria immitis.

    abstract::Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mendis AH,Rose ME,Rees HH,Goodwin TW

    更新日期:1983-11-01 00:00:00

  • Characterization of tubulin genes in Trypanosoma rangeli.

    abstract::Tubulin genes in Trypanosoma rangeli, the only trypanosome besides T. cruzi to infect humans in America, are organized in homogeneous, alternate alpha and beta gene tandem repeats of 3.8 kb. The basic repeat was cloned, mapped and partially sequenced. In contrast to most other eukaryotes, where tubulin genes are scatt...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Esquenazi D,Morel CM,Traub-Cseko YM

    更新日期:1989-05-15 00:00:00

  • Membrane modifications in erythrocytes parasitized by Plasmodium falciparum.

    abstract::Plasmodium falciparum malaria parasites invade human red blood cells and immediately begin making significant alterations to the structure of the erythrocyte. These alterations facilitate the movement of nutrients into, and waste products and parasite-derived proteins out of the cell to meet the needs of the growing p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Deitsch KW,Wellems TE

    更新日期:1996-02-01 00:00:00

  • Surface-associated antigens of Brugia malayi L2 and L3 parasites during vector-stage development.

    abstract::Surface and metabolic labeling procedures were used to characterize the composition and the time of expression of Brugia malayi L2 and L3 surface-associated molecules as the larvae develop within the mosquito vector. Larvae were harvested from mosquito tissues at 5 (early L2), 8 (late L2) and 11 (L3) days post-infecti...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ibrahim MS,Richie TL,Scott AL

    更新日期:1992-05-01 00:00:00

  • Rapid evolution of an erythrocyte invasion gene family: the Plasmodium reichenowi Reticulocyte Binding Like (RBL) genes.

    abstract::Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rayner JC,Huber CS,Galinski MR,Barnwell JW

    更新日期:2004-02-01 00:00:00

  • An S antigen gene from Plasmodium falciparum contains a novel repetitive sequence.

    abstract::The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

    更新日期:1988-02-01 00:00:00

  • A (Ca(2+)-Mg2+)ATPase from Schistosoma mansoni is coupled to an active transport of calcium.

    abstract::The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cunha VM,Meyer-Fernandes JR,Noël F

    更新日期:1992-06-01 00:00:00

  • The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis.

    abstract::The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

    更新日期:1988-10-01 00:00:00

  • An epitope recognised by inhibitory monoclonal antibodies that react with a 51 kilodalton merozoite surface antigen in Plasmodium falciparum.

    abstract::Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

    更新日期:1988-02-01 00:00:00

  • The Leishmania promastigote surface antigen 2 complex is differentially expressed during the parasite life cycle.

    abstract::The promastigote surface antigen 2 (PSA-2) complex comprises a family of antigenically similar polypeptides of M(r) 96,000, 80,000 and 50,000, anchored to the membrane with glycosylphosphatidylinositol. Although PSA-2 was initially detected only in promastigotes, Northern blot analysis indicated that mRNA transcripts ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Osborn AH,Symons F,van Driel R,Cappai R

    更新日期:1995-11-01 00:00:00

  • Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis.

    abstract::Leucine aminopeptidases (LAP; EC are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

    更新日期:2012-03-01 00:00:00

  • Protoporphyrinogen IX oxidase from Plasmodium falciparum is anaerobic and is localized to the mitochondrion.

    abstract::Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

    更新日期:2010-11-01 00:00:00

  • An exported protein of Plasmodium falciparum is synthesized as an integral membrane protein.

    abstract::Exp-1 is an antigen of Plasmodium falciparum which is transported from the parasite cell to the membrane of the parasitophorous vacuole and to membranous compartments in the erythrocyte. To investigate how this protein is transported, we studied the synthesis and membrane translocation of exp-1 in a cell-free system. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Günther K,Tümmler M,Arnold HH,Ridley R,Goman M,Scaife JG,Lingelbach K

    更新日期:1991-05-01 00:00:00

  • Similarity of a 16.5kDa tegumental protein of the human liver fluke Opisthorchis viverrini to nematode cytoplasmic motility protein.

    abstract::Opisthorchis viverrini is the causative agent of human opisthorchiasis in Thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. In this work we have molecularly characterized the first member of a protein family carrying two DM9 repeats in this parasite (...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Labbunruang N,Phadungsil W,Tesana S,Smooker PM,Grams R

    更新日期:2016-05-01 00:00:00

  • Substrate specificity of recombinant cysteine proteinase, CPB, of Leishmania mexicana.

    abstract::The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

    更新日期:2001-08-01 00:00:00

  • A surface-labeled 18 kilodalton antigen in Schistosoma mansoni.

    abstract::A mild surface-labeling procedure was applied to various developmental stages of Schistosoma mansoni. An 18 kDa protein was preferentially labeled in freshly transformed schistosomula. The labeled protein was equally present on skin-penetrated and mechanically prepared schistosomula and it disappeared upon digestion o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liberti P,Festucci A,Ruppel A,Gigante S,Cioli D

    更新日期:1986-01-01 00:00:00

  • The Leishmania mexicana proteasome.

    abstract::As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Robertson CD

    更新日期:1999-09-20 00:00:00

  • Characterization of a Fasciola gigantica protein carrying two DM9 domains reveals cellular relocalization property.

    abstract::Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

    更新日期:2016-01-01 00:00:00

  • Identification and characterization of a Plasmodium falciparum RNA polymerase gene with similarity to mitochondrial RNA polymerases.

    abstract::Nearly all mitochondrial RNA polymerase genes identified to date are encoded in the nucleus and have similarities to T3 and T7 bacteriophage RNA polymerases. Some chloroplast genes are also transcribed by T3/T7 phage-like RNA polymerases, raising the possibility that the apicomplexan parasites, which have both a mitoc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Li J,Maga JA,Cermakian N,Cedergren R,Feagin JE

    更新日期:2001-04-06 00:00:00

  • Studies of the effect of diamphenethide and oxyclozanide on the metabolism of Fasciola hepatica.

    abstract::Studies were made of the effects of diamphenethide-amine on glucose transport, glycogen breakdown, adenine nucleotides, metabolites and excretory products in both parenchymal and bile duct flukes in vitro and in bile duct flukes in vivo. The most consistent and pronounced effect observed was an elevation of malate lev...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Edwards SR,Campbell AJ,Sheers M,Moore RJ,Montague PE

    更新日期:1981-04-01 00:00:00

  • Molecular cloning of a myoD-like gene from the parasitic nematode, Trichinella spiralis.

    abstract::The infective larval stage of the nematode Trichinella spiralis is an intracellular parasite of skeletal muscle cells. Infection with T. spiralis results in dedifferentiation of the host cell and the formation of a host/parasite complex. A gene encoding a T. spiralis Helix-Loop-helix (HLH) protein with homology to the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Connolly B,Trenholme K,Smith DF

    更新日期:1996-10-30 00:00:00

  • In silico identification of conserved intercoding sequences in Leishmania genomes: unraveling putative cis-regulatory elements.

    abstract::In silico analyses of Leishmania spp. genome data are a powerful resource to improve the understanding of these pathogens' biology. Trypanosomatids such as Leishmania spp. have their protein-coding genes grouped in long polycistronic units of functionally unrelated genes. The control of gene expression happens by a va...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Vasconcelos EJ,Terrão MC,Ruiz JC,Vêncio RZ,Cruz AK

    更新日期:2012-06-01 00:00:00

  • Purification and characterization of three larval excretory-secretory proteins of Dirofilaria immitis.

    abstract::Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Frank GR,Grieve RB

    更新日期:1996-01-01 00:00:00

  • Assessment of pfmdr 1 gene copy number by tandem competitive polymerase chain reaction.

    abstract::The pfmdr 1 gene encodes a Plasmodium falciparum homologue of the human P-glycoprotein expressed on the surface of the parasite food vacuole. Variation in copy number and specific codon mutations of pfmdr 1 have been implicated in the development of parasite resistance to antimalarial drugs. We describe here the techn...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Price R,Robinson G,Brockman A,Cowman A,Krishna S

    更新日期:1997-04-01 00:00:00

  • DNA sequence of Crithidia fasciculata kinetoplast minicircles.

    abstract::Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sugisaki H,Ray DS

    更新日期:1987-04-01 00:00:00

  • Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach.

    abstract::Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

    更新日期:2021-01-07 00:00:00