Protein variation in clones of Plasmodium falciparum detected by two dimensional electrophoresis.

abstract：:Kinetoplast DNA (kDNA) was isolated from 56 stocks of Trypanosoma cruzi isolated from human patients, animals and insects from Brazil, Venezuela, Colombia and Costa Rica. Comparison of the patterns of digested kDNA on acrylamide gels led to the grouping of several stocks into two schizodemes. Schizodeme analysis was a...

journal_title：Molecular and biochemical parasitology

authors： Avila H,Goncalves AM,Nehme NS,Morel CM,Simpson L

更新日期：1990-09-01 00:00:00

abstract：:Ribosomal RNA isolated from the malaria parasite Plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 X 10(6) and 0.78 +/- 0.02 X 10(6) present in equimolar quantities. Their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. T...

journal_title：Molecular and biochemical parasitology

authors： Hyde JE,Zolg JW,Scaife JG

更新日期：1981-12-31 00:00:00

abstract：:The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

journal_title：Molecular and biochemical parasitology

authors： Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

更新日期：2016-01-01 00:00:00

abstract：:Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

journal_title：Molecular and biochemical parasitology

authors： Das A

更新日期：1993-08-01 00:00:00

abstract：:In trypanosomatids, removal of hydrogen peroxide and other aryl and alkyl peroxides is achieved by the NADPH-dependent trypanothione peroxidase system, whose components are trypanothione reductase (TRYR), trypanothione, tryparedoxin (TRYX) and tryparedoxin peroxidase (TRYP). Here, we report the cloning of a multi-copy...

journal_title：Molecular and biochemical parasitology

authors： Tetaud E,Giroud C,Prescott AR,Parkin DW,Baltz D,Biteau N,Baltz T,Fairlamb AH

更新日期：2001-09-03 00:00:00

abstract：:A membrane-bound sialidase (EC 3.2.1.18) was found in procyclic trypomastigotes of Trypanosoma brucei. The mammalian stage bloodstream form, however, displayed no sialidase activity. This sialidase is an integral surface protein, linked to the membrane via a glycosylphosphatidylinositol anchor. After osmotic lysis and...

journal_title：Molecular and biochemical parasitology

authors： Engstler M,Reuter G,Schauer R

更新日期：1992-08-01 00:00:00

abstract：:A Trypanosoma cruzi small subunit ribosomal RNA gene was sequenced from genomic recombinant plasmid clones. The assigned coding region was 2319 bp, the longest SSU rRNA gene described to date. On the basis of comparisons with published sequences from Crithidia fasciculata, Trypanosoma brucei, and Leishmania donovani, ...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Rios P,Valdés AM,Piñero D

更新日期：1990-06-01 00:00:00

abstract：:During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

journal_title：Molecular and biochemical parasitology

authors： Martinez SL,Clavijo CA,Winograd E

更新日期：1998-03-15 00:00:00

abstract：:Apicomplexan parasites utilize a peripheral membrane system called the inner membrane complex (IMC) to facilitate host cell invasion and parasite replication. We recently identified a novel family of Toxoplasma IMC Sub-compartment Proteins (ISP1/2/3) that localize to sub-domains of the IMC using a targeting mechanism ...

journal_title：Molecular and biochemical parasitology

authors： Fung C,Beck JR,Robertson SD,Gubbels MJ,Bradley PJ

更新日期：2012-08-01 00:00:00

abstract：:Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

journal_title：Molecular and biochemical parasitology

authors： Debrabant A,Delplace P

更新日期：1989-03-01 00:00:00

abstract：:Theileria annulata is a tick-transmitted protozoan that causes tropical theileriosis, an often fatal leukoproliferative disorder of cattle. To characterize and identify parasite proteins suitable as diagnostic antigens and/or vaccine candidates, a cDNA clone encoding a macroschizont stage protein was isolated and char...

journal_title：Molecular and biochemical parasitology

authors： Schnittger L,Katzer F,Biermann R,Shayan P,Boguslawski K,McKellar S,Beyer D,Shiels BR,Ahmed JS

更新日期：2002-04-09 00:00:00

abstract：:Investigating the proteome of intracellular Leishmania amastigotes has recently become possible due to the exploitation of fluorescence activated intracellular parasite sorting. Here, we employed this technology in combination with gel free analysis to greatly improve proteome coverage and suggest proteins putatively ...

journal_title：Molecular and biochemical parasitology

authors： Paape D,Barrios-Llerena ME,Le Bihan T,Mackay L,Aebischer T

更新日期：2010-02-01 00:00:00

abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

journal_title：Molecular and biochemical parasitology

authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

更新日期：2010-10-01 00:00:00

abstract：:A Fasciola hepatica cDNA clone of 1752 bp was isolated from an adult worm cDNA expression library by immunological screening using a rabbit serum against the excretory-secretory antigens. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 489 codons which encoded a 55 kDa polypeptide...

journal_title：Molecular and biochemical parasitology

authors： Salazar-Calderón M,Martín-Alonso JM,Castro AM,Parra F

更新日期：2003-01-01 00:00:00

abstract：:Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

journal_title：Molecular and biochemical parasitology

authors： Engelmann S,Sinnis P,Matuschewski K

更新日期：2006-03-01 00:00:00

abstract：:It was found that the digenetic trypanosomatid Endotrypanum schaudinni transferred Man7GlcNAc2 in protein N-glycosylation. Endo-beta-N-acetylglucosaminidase H-sensitive oligosaccharides were identified as Man7GlcNAc2, Man6GlcNAc2, Rib1Man6GlcNAc2 and/or Gal1Man6GlcNAc2, Man5GlcNAc containing two galactose or ribose un...

journal_title：Molecular and biochemical parasitology

authors： Merello S,Xavier MT,Parodi AJ

更新日期：1995-01-01 00:00:00

abstract：:The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties...

journal_title：Molecular and biochemical parasitology

authors： Wan KL,Carruthers VB,Sibley LD,Ajioka JW

更新日期：1997-02-01 00:00:00

abstract：:The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to ...

journal_title：Molecular and biochemical parasitology

authors： Kangussu-Marcolino MM,Cunha AP,Avila AR,Herman JP,DaRocha WD

更新日期：2014-12-01 00:00:00

abstract：:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

journal_title：Molecular and biochemical parasitology

authors： Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

更新日期：2020-07-01 00:00:00

abstract：:Neospora caninum is an obligate intracellular Apicomplexa, a phylum where one of the current methods for functional studies relies on molecular genetic tools. For Toxoplasma gondii, the first method described, in 1993, was based on resistance against chloramphenicol. As in T. gondii, we developed a vector constituted ...

journal_title：Molecular and biochemical parasitology

authors： Pereira LM,Yatsuda AP

更新日期：2014-09-01 00:00:00

abstract：:A novel Plasmodium falciparum sporozoite antigen, STARP (Sporozoite Threonine and Asparagine-Rich Protein), detected consistently on the surface of sporozoites obtained from laboratory strains and field isolates, has been identified and cloned, following a systematic approach aimed at isolating novel non-CS sporozoite...

journal_title：Molecular and biochemical parasitology

authors： Fidock DA,Bottius E,Brahimi K,Moelans II,Aikawa M,Konings RN,Certa U,Olafsson P,Kaidoh T,Asavanich A

更新日期：1994-04-01 00:00:00

abstract：:Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

journal_title：Molecular and biochemical parasitology

authors： Pampori NA,Singh G,Srivastava VM

更新日期：1984-04-01 00:00:00

abstract：:The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

journal_title：Molecular and biochemical parasitology

authors： Wang J,Böhme U,Cross GA

更新日期：2003-05-01 00:00:00

abstract：:The bloodstream forms of the protozoan parasite Trypanosoma brucei lack spectrally detectable cytochromes and satisfy energy requirements mainly by glycolysis. When infected blood is ingested by the tse-tse fly vector, the bloodstream form cells differentiate to procyclic forms that have fully functional mitochondria....

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Hajduk SL

更新日期：1994-06-01 00:00:00

abstract：:Seven beta-naphthylamine-linked peptides were tested as substrates for a previously described thiol proteinase of adult Schistosoma mansoni. The enzyme was not active on carbobenoxy-arginyl-arginyl-4-methoxy-2-naphthylamide and carbobenzoxy-alanyl-arginyl-arginyl-4-methoxy-2-naphthylamide. Enzyme activity was maximal ...

journal_title：Molecular and biochemical parasitology

authors： Dresden MH,Rutledge ML,Chappell CL

更新日期：1981-11-01 00:00:00

abstract：:Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

journal_title：Molecular and biochemical parasitology

authors： Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

更新日期：1990-11-01 00:00:00

abstract：:Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

journal_title：Molecular and biochemical parasitology

authors： Walker R Jr,Saha L,Hill GC,Chaudhuri M

更新日期：2005-02-01 00:00:00

abstract：:We have isolated a 0.3-kb HaeIII restriction fragment from Trypanosoma brucei which contains two tRNA genes. Secondary structure models predict that the two genes identified encode tRNA molecules which specify glycine (anticodon UCC) and leucine (anticodon CAG). The two genes are separated by 86 nucleotides, transcrib...

journal_title：Molecular and biochemical parasitology

authors： Campbell DA,Suyama Y,Simpson L

更新日期：1989-12-01 00:00:00

abstract：:Malaria parasites possess a de novo heme synthetic pathway. Interestingly, this pathway is dispensable during the blood stages of development in mammalian hosts. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochro...

journal_title：Molecular and biochemical parasitology

authors： Posayapisit N,Songsungthong W,Koonyosying P,Falade MO,Uthaipibull C,Yuthavong Y,Shaw PJ,Kamchonwongpaisan S

更新日期：2016-01-01 00:00:00

abstract：:Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recent...

journal_title：Molecular and biochemical parasitology

authors： Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

更新日期：1999-03-15 00:00:00