The P-glycoprotein-related gene family in Leishmania.

abstract：:We have demonstrated that bradyzoites return to the tissue-cyst stage by a developmental pathway that is indistinguishable from that initiated by sporozoites. Mature bradyzoites, like sporozoites from oocysts, were non-proliferative as they contained uniform 1N DNA contents, and replication occurred only in parasites ...

journal_title：Molecular and biochemical parasitology

authors： Radke JR,Guerini MN,Jerome M,White MW

更新日期：2003-10-01 00:00:00

abstract：:Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

journal_title：Molecular and biochemical parasitology

authors： Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

更新日期：2009-11-01 00:00:00

abstract：:Phosphatidylcholine (PC) is the most abundant phospholipid in the membranes of the human parasite Leishmania. It is synthesized via two metabolic routes, the de novo pathway that starts with the uptake of choline, and the threefold methylation of phosphatidylethanolamine. Choline was shown to be dispensable for Leishm...

journal_title：Molecular and biochemical parasitology

authors： Bibis SS,Dahlstrom K,Zhu T,Zufferey R

更新日期：2014-09-01 00:00:00

abstract：:Schistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut...

journal_title：Molecular and biochemical parasitology

authors： Mathieson W,Castro-Borges W,Wilson RA

更新日期：2011-02-01 00:00:00

abstract：:The synchronization of intraerythrocytic maturation of Plasmodium parasites is an important factor in the malaria infection process. Synchronization is mediated by inositol phosphate (InsP(x))-induced Ca(2+)-release from internal stores. To further investigate the InsP(x) metabolism in these parasites a Plasmodium pro...

journal_title：Molecular and biochemical parasitology

authors： Stritzke C,Nalaskowski MM,Fanick W,Lin H,Mayr GW

更新日期：2012-12-01 00:00:00

abstract：:Schistosomiasis is an intravascular parasitic infection estimated to affect over 206 million people, the majority of whom live in Africa where the trematode worms Schistosoma mansoni and Schistosoma haematobium are the major causative agents. While a number of drugs have been used to treat schistosomiasis, praziquante...

journal_title：Molecular and biochemical parasitology

authors： Sanchez MC,Cupit PM,Bu L,Cunningham C

更新日期：2019-03-01 00:00:00

abstract：:The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

journal_title：Molecular and biochemical parasitology

authors： Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

更新日期：2001-03-01 00:00:00

abstract：:We report the isolation and sequence of a part of a gene encoding a collagen from the genome of the human filarial parasite Brugia malayi. The deduced amino acid sequence of the sequenced portion of this gene, which we have designated BmCol1, differs from the most catalogued nematode collagens in that it is composed p...

journal_title：Molecular and biochemical parasitology

authors： Caulagi VR,Werner C,Rajan TV

更新日期：1991-03-01 00:00:00

abstract：:Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoites store TLN4 in the micronemes and secrete it in response to elevated calcium, ...

journal_title：Molecular and biochemical parasitology

authors： Laliberté J,Carruthers VB

更新日期：2011-05-01 00:00:00

abstract：:The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of pu...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Galatis D,Cowman AF

更新日期：1993-04-01 00:00:00

abstract：:Leishmania major aquaglyceroporin (AQP1) is an adventitious metalloid channel that allows the bidirectional movement of arsenite and antimonite. Here we demonstrate that AQP1 is subjected to proteasome-dependent degradation. Treatment of Leishmania promastigotes with the proteasome inhibitor MG132 resulted in increase...

journal_title：Molecular and biochemical parasitology

authors： Sharma M,Mandal G,Mandal S,Bhattacharjee H,Mukhopadhyay R

更新日期：2015-06-01 00:00:00

abstract：:To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Van Roy J

更新日期：1982-09-01 00:00:00

abstract：:We have initiated a project to characterize telomere-associated proteins of Cryptosporidium parvum. Searching public databases with C. parvum expressed sequence tag (EST) sequences revealed one EST sequence that is highly similar to Gbp1p of Chlamydomonas reinhardtii (Cr Gbp1p), a protein that binds single-stranded te...

journal_title：Molecular and biochemical parasitology

authors： Liu C,Wang L,Lancto CA,Abrahamsen MS

更新日期：2009-06-01 00:00:00

abstract：:A protease from Plasmodium falciparum was purified 150 fold by high performance liquid chromatography on a TSK-G-3000 SW exclusion column. The enzyme is not retained during pressure filtration with an Amicon PM10 membrane but is retained by a YM5 membrane. The molecular weight of the protease is less than 10,000, base...

journal_title：Molecular and biochemical parasitology

authors： Vander Jagt DL,Hunsaker LA,Campos NM

更新日期：1986-03-01 00:00:00

abstract：:A restriction fragment from a cloned kinetoplast minicircle DNA has been shown to be diagnostic for Leishmania major. This 402-bp TaqI fragment has been used routinely (as a radiolabelled probe) to detect 10(4) parasites in simple dot blots, both experimentally and in epidemiological surveys. It positively identified ...

journal_title：Molecular and biochemical parasitology

authors： Smith DF,Searle S,Ready PD,Gramiccia M,Ben-Ismail R

更新日期：1989-12-01 00:00:00

abstract：:Entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. Parasite vesicle trafficking and motility, which relies on vesicle trafficking to deliver membrane and membrane components to the leading edge, are important for viru...

journal_title：Molecular and biochemical parasitology

authors： Welter BH,Temesvari LA

更新日期：2004-06-01 00:00:00

abstract：:Studies measuring the glycolytic intermediate and adenine nucleotide concentrations in Trypanosoma brucei metabolising glucose either aerobically or under conditions where glycerol-3-phosphate oxidase is inactive have shown the following: 1. Inhibition with 0.5 mM salicylhydroxamic acid (SHAM) accurately simulates ana...

journal_title：Molecular and biochemical parasitology

authors： Hammond DJ,Bowman IB

更新日期：1980-12-01 00:00:00

abstract：:Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism bi...

journal_title：Molecular and biochemical parasitology

authors： Jiang L,Duriseti S,Sun P,Miller LH

更新日期：2009-11-01 00:00:00

abstract：:The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions hav...

journal_title：Molecular and biochemical parasitology

authors： Sonda S,Fuchs N,Connolly B,Fernandez P,Gottstein B,Hemphill A

更新日期：1998-11-30 00:00:00

abstract：:Entamoeba histolytica is a eukaryotic intestinal parasite of humans, and is endemic in developing countries. We have characterized the E. histolytica putative low molecular weight protein tyrosine phosphatase (LMW-PTP). The structure for this amebic tyrosine phosphatase was solved, showing the ligand-induced conformat...

journal_title：Molecular and biochemical parasitology

authors： Linford AS,Jiang NM,Edwards TE,Sherman NE,Van Voorhis WC,Stewart LJ,Myler PJ,Staker BL,Petri WA Jr

更新日期：2014-01-01 00:00:00

abstract：:Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vacci...

journal_title：Molecular and biochemical parasitology

authors： Clarke LE,Messer LI,Greenwood NM,Wisher MH

更新日期：1987-01-02 00:00:00

abstract：:Antisera from lambs immunised with the Haemonchus contortus integral membrane protein complex, Haemonchus galactose-containing glycoprotein (H-gal-GP), the lambs being refractory to subsequent challenge, were used to identify several clones from an adult H. contortus lambda gt11 cDNA library. Using gene-specific oligo...

journal_title：Molecular and biochemical parasitology

authors： Redmond DL,Knox DP,Newlands G,Smith WD

更新日期：1997-03-01 00:00:00

abstract：:Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

journal_title：Molecular and biochemical parasitology

authors： Polinski M,Hamilton DB,Nowak B,Bridle A

更新日期：2013-09-01 00:00:00

abstract：:The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We hav...

journal_title：Molecular and biochemical parasitology

authors： Lahti CJ,Bradley PJ,Johnson PJ

更新日期：1994-08-01 00:00:00

abstract：:Two short DNA segments were isolated by screening of a lambda gt11 library from Plasmodium falciparum schizont cDNA with an antiserum against the 140 kDa protein, which confers protective immunity to monkeys. The segments were used to identify a genomic fragment which carries the entire coding sequence for a protein o...

journal_title：Molecular and biochemical parasitology

authors： Knapp B,Hundt E,Nau U,Küpper HA

更新日期：1989-01-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence anal...

journal_title：Molecular and biochemical parasitology

authors： Barnes DA,Bonnin A,Huang JX,Gousset L,Wu J,Gut J,Doyle P,Dubremetz JF,Ward H,Petersen C

更新日期：1998-10-30 00:00:00

abstract：:Cryptosporidium spp. are waterborne apicomplexan parasites responsible for outbreaks of diarrheal disease worldwide. Antigens involved in zoite invasion into host cells have been the focus of many investigations as these may prove to be good vaccine candidates. gp40/15 is a zoite antigen synthesized as a precursor pro...

journal_title：Molecular and biochemical parasitology

authors： O'Connor RM,Wanyiri JW,Cevallos AM,Priest JW,Ward HD

更新日期：2007-11-01 00:00:00

abstract：:Trichuris arvicolae and T. muris are gastro-intestinal nematodes of respectively arvicoline and murine rodents. We aim to investigate the ecology of these Trichuris species using population genetics. We sampled nematodes from rodents trapped in the East of France. After confirming the species identification of the nem...

journal_title：Molecular and biochemical parasitology

authors： Deter J,Galan M,Charbonnel N,Cosson JF

更新日期：2009-10-01 00:00:00

abstract：:An axenic cultivation system was used to study the differentiation of Trypanosoma brucei bloodstream forms from long slender to short stumpy-like forms. Trypanosomes in the logarithmic phase are similar to long slender bloodstream forms freshly isolated from infected mice, differing only in the rate of oxygen uptake. ...

journal_title：Molecular and biochemical parasitology

authors： Hamm B,Schindler A,Mecke D,Duszenko M

更新日期：1990-04-01 00:00:00

abstract：:Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

journal_title：Molecular and biochemical parasitology

authors： Cabrera Z,Parkhouse RM

更新日期：1986-09-01 00:00:00