Identification of novel splice variants of the voltage- and Ca²⁺-dependent K⁺-channel SLO-1 of Trichuris muris.


:The anthelmintic cyclooctadepsipeptide emodepside is effective against nematodes showing resistance against established drug classes. Emodepside exerts its nematicidal effects mainly through its validated target, the tetrameric voltage- and calcium-activated potassium channel SLO-1. Two slo-1 genes were described in Trichuris muris. Alternative splicing is known to alter SLO-1 properties. Here, 16 T. muris splice variants for slo-1.1 and three variants for slo-1.2 were identified in addition to previously described variants. Splice variants caused by intron retentions and/or exon exclusions encode varyingly truncated subunits. Depending on the subunit composition, channels might have altered physiological and pharmacological properties including different modulation by calcium and/or voltage or reduced emodepside susceptibility which might lead to emodepside resistance as observed in Caenorhabditis elegans expressing only similarly truncated Slo-1. The comprehensive characterisation of splice variants is a prerequisite for functional analysis and confirmed conservation of remarkable differences found between both slo-1 paralogs in Trichuris suis.


Mol Biochem Parasitol


Yilmaz E,Kulke D,von Samson-Himmelstjerna G,Krücken J




Has Abstract


2015-01-01 00:00:00














  • The effects of p-chloromercuriphenylsulfonic acid on Trypanosoma cruzi infection of mammalian host cells in vitro.

    abstract::Treatment of Trypanosoma cruzi blood trypomastigotes with p-chloromercuriphenylsulfonic acid (PCMS) increased the association of the parasite with either mouse resident peritoneal macrophages or rat heart myoblasts in vitro. The effect was evidenced by elevation of both the percentage of host cells with parasites and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ayala A,Kierszenbaum F

    更新日期:1987-02-01 00:00:00

  • Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia.

    abstract::Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adedoja AN,McMahan T,Neal JP,Hamal Dhakal S,Jois S,Romo D,Hull K,Garlapati S

    更新日期:2020-03-01 00:00:00

  • Proteomic analysis of Toxocara canis excretory and secretory (TES) proteins.

    abstract::Toxocariasis is a neglected disease, and its main etiological agent is the nematode Toxocara canis. Serological diagnosis is performed by an enzyme-linked immunosorbent assay using T. canis excretory and secretory (TES) antigens produced by in vitro cultivation of larvae. Identification of TES proteins can be useful f...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sperotto RL,Kremer FS,Aires Berne ME,Costa de Avila LF,da Silva Pinto L,Monteiro KM,Caumo KS,Ferreira HB,Berne N,Borsuk S

    更新日期:2017-01-01 00:00:00

  • Isolation, characterization and analysis of the expression of the Leishmania ribosomal PO protein genes.

    abstract::Two tandemly linked genes are present in the Leishmania infantum genome that code for the acidic ribosomal PO protein. The genes are identical in the coding region, although a striking lack of nucleotide sequence conservation is observed when the boundaries of the coding regions between both genes are compared. The 3'...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Soto M,Requena JM,Alonso C

    更新日期:1993-10-01 00:00:00

  • Energy metabolism in Cotugnia digonopora and the effect of anthelmintics.

    abstract::Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pampori NA,Singh G,Srivastava VM

    更新日期:1984-04-01 00:00:00

  • Expression and functional characterization of a giant Type I fatty acid synthase (CpFAS1) gene from Cryptosporidium parvum.

    abstract::A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

    更新日期:2004-03-01 00:00:00

  • Extensive homologies between Leishmania donovani chromosomes of markedly different size.

    abstract::The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bishop RP

    更新日期:1990-01-01 00:00:00

  • Sequences required for the flagellar targeting of an integral membrane protein.

    abstract::Previous studies have established that the ISO1 glucose transporter of Leishmania enriettii resides primarily in the flagellar membrane, whereas the ISO2 glucose transporter is located in the pellicular plasma membrane surrounding the cell body. This pronounced difference in subcellular targeting is conferred by the N...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nasser MI,Landfear SM

    更新日期:2004-05-01 00:00:00

  • An S antigen gene from Plasmodium falciparum contains a novel repetitive sequence.

    abstract::The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

    更新日期:1988-02-01 00:00:00

  • The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1.

    abstract::Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are larg...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Shakya B,Penn WD,Nakayasu ES,LaCount DJ

    更新日期:2017-09-01 00:00:00

  • Cloning and sequencing of an hsp70 gene of Schistosoma mansoni.

    abstract::Schistosomes have a complex life cycle (vertebrate and molluscan hosts as well as larvae living freely in water) in which they are exposed to different environments and temperatures (20 degrees C - 37 degrees C). Since heat shock genes are activated in response to stress and during development [1], it is of interest t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Neumann S,Ziv E,Lantner F,Schechter I

    更新日期:1992-12-01 00:00:00

  • Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach.

    abstract::Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

    更新日期:2021-01-07 00:00:00

  • The complete sequence of a Plasmodium malariae SSUrRNA gene and its comparison to other plasmodial SSUrRNA genes.

    abstract::A gene encoding the small subunit rRNA (SSUrRNA) has been isolated from the human parasite, Plasmodium malariae. The gene has been sequenced. It contains conserved and variable regions which conform to patterns established for other eukaryotic SSUrRNA genes. Comparisons with other SSUrRNA genes from Plasmodium species...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Goman M,Mons B,Scaife J

    更新日期:1991-04-01 00:00:00

  • The effect of over-expression of the alternative oxidase in the procyclic forms of Trypanosoma brucei.

    abstract::Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Walker R Jr,Saha L,Hill GC,Chaudhuri M

    更新日期:2005-02-01 00:00:00

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    abstract::Surface and metabolic labeling procedures were used to characterize the composition and the time of expression of Brugia malayi L2 and L3 surface-associated molecules as the larvae develop within the mosquito vector. Larvae were harvested from mosquito tissues at 5 (early L2), 8 (late L2) and 11 (L3) days post-infecti...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ibrahim MS,Richie TL,Scott AL

    更新日期:1992-05-01 00:00:00

  • Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Matrangolo FS,Liarte DB,Andrade LC,de Melo MF,Andrade JM,Ferreira RF,Santiago AS,Pirovani CP,Silva-Pereira RA,Murta SM

    更新日期:2013-08-01 00:00:00

  • Molecular species analysis and quantification of the glycosylphosphatidylinositol intermediate glycolipid C from Trypanosoma brucei.

    abstract::The precursor of the glycosylphosphatidylinositol membrane anchor of the variant surface glycoprotein of Trypanosoma brucei is known as glycolipid A and it has the structure: EtN-PO4-6Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol-PO4-(sn-1,2-dimyristoylglycerol). This precursor exists in equilibriu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Güther ML,Treumann A,Ferguson MA

    更新日期:1996-05-01 00:00:00

  • Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii.

    abstract::Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suvorova ES,Lehmann MM,Kratzer S,White MW

    更新日期:2012-01-01 00:00:00

  • Transient expression of luciferase in Entamoeba histolytica driven by the ferredoxin gene 5' and 3' regions.

    abstract::We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gilchrist CA,Streets HL,Ackers JP,Hall R

    更新日期:1995-10-01 00:00:00

  • A Trypanosoma brucei mutant resistant to alpha-difluoromethylornithine.

    abstract::Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phillips MA,Wang CC

    更新日期:1987-01-02 00:00:00

  • In vivo 31P NMR spectrum of Hymenolepis diminuta and its change on short-term exposure to mebendazole.

    abstract::The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Thompson SN,Platzer EG,Lee RW

    更新日期:1987-01-02 00:00:00

  • Purification and partial characterization of an unusual protein of Plasmodium falciparum: histidine-rich protein II.

    abstract::The human malarial parasite Plasmodium falciparum secretes a histidine-rich protein (HRP-II) from infected erythrocytes. HRP-II has a very high content of histidine (H) (34%), alanine (A) (37%) and aspartic acid (D) (10%) and many contiguous repeats of the sequences AHH and AHHAAD. The histidine content of the protein...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Panton LJ,McPhie P,Maloy WL,Wellems TE,Taylor DW,Howard RJ

    更新日期:1989-06-15 00:00:00

  • Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni.

    abstract::The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Aragon AD,Imani RA,Blackburn VR,Cunningham C

    更新日期:2008-12-01 00:00:00

  • Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole.

    abstract::In the present work, we evaluate in vitro the cytosolic and microsomal activity of glutathione-S-transferases in adults of Fasciola hepatica susceptible (Cullompton Strain) and resistant (Sligo Strain) to triclabendazole. The triclabendazole resistant (Sligo) fluke expressed significant major metabolic activity of glu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scarcella S,Lamenza P,Virkel G,Solana H

    更新日期:2012-01-01 00:00:00

  • Molecular characterization and expression of two putative protective excretory secretory proteins of Haemonchus contortus.

    abstract::It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

    更新日期:1997-09-01 00:00:00

  • Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti.

    abstract::Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KD

    更新日期:2009-12-01 00:00:00

  • Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate.

    abstract::The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated b...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Perry DA,Morrison HG,Adam RD

    更新日期:2011-12-01 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • Studies on mitochondrial ATPase of Leishmania donovani using digitonin-permeabilized promastigotes.

    abstract::Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Das A

    更新日期:1993-08-01 00:00:00

  • Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex.

    abstract::A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Webster P,Mansour TE,Bieber D

    更新日期:1989-10-01 00:00:00