Protection by phospholipids of Schistosoma mansoni schistosomula against the action of cytotoxic antibodies and complement.

abstract：:Trypanosoma brucei survives in mammals by antigenic variation of its surface coat consisting of variant surface glycoprotein (VSG). Trypanosomes change coat mainly by replacing the transcribed VSG genes in an active telomeric expression site by a different VSG gene. There are about 20 different expression sites and tr...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Ulbert S

更新日期：2001-04-25 00:00:00

abstract：:In the present work, we evaluate in vitro the cytosolic and microsomal activity of glutathione-S-transferases in adults of Fasciola hepatica susceptible (Cullompton Strain) and resistant (Sligo Strain) to triclabendazole. The triclabendazole resistant (Sligo) fluke expressed significant major metabolic activity of glu...

journal_title：Molecular and biochemical parasitology

authors： Scarcella S,Lamenza P,Virkel G,Solana H

更新日期：2012-01-01 00:00:00

abstract：:The Leishmania GP63 major surface protein gene family encodes multiple isoforms which differ predominantly in the carboxyterminal region. We have isolated 4 full-length gp63 cDNA clones derived from stationary-phase promastigote RNA of a cloned isolate of Leishmania guyanensis, a member of the braziliensis complex. Th...

journal_title：Molecular and biochemical parasitology

authors： Steinkraus HB,Greer JM,Stephenson DC,Langer PJ

更新日期：1993-12-01 00:00:00

abstract：:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

journal_title：Molecular and biochemical parasitology

authors： Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

更新日期：1990-12-01 00:00:00

abstract：:Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the he...

journal_title：Molecular and biochemical parasitology

authors： Ntambi JM,Marini JC,Bangs JD,Hajduk SL,Jimenez HE,Kitchin PA,Klein VA,Ryan KA,Englund PT

更新日期：1984-07-01 00:00:00

abstract：:Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/P...

journal_title：Molecular and biochemical parasitology

authors： Garcia-Silva MR,Frugier M,Tosar JP,Correa-Dominguez A,Ronalte-Alves L,Parodi-Talice A,Rovira C,Robello C,Goldenberg S,Cayota A

更新日期：2010-06-01 00:00:00

abstract：:The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

journal_title：Molecular and biochemical parasitology

authors： Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

更新日期：1995-02-01 00:00:00

abstract：:Seven beta-naphthylamine-linked peptides were tested as substrates for a previously described thiol proteinase of adult Schistosoma mansoni. The enzyme was not active on carbobenoxy-arginyl-arginyl-4-methoxy-2-naphthylamide and carbobenzoxy-alanyl-arginyl-arginyl-4-methoxy-2-naphthylamide. Enzyme activity was maximal ...

journal_title：Molecular and biochemical parasitology

authors： Dresden MH,Rutledge ML,Chappell CL

更新日期：1981-11-01 00:00:00

abstract：:Precipitin arcs formed after immunoelectrophoresis of Schistosoma mansoni egg, worm and cercarial antigens with polyspecific rabbit antisera have been 'stained' at neutral pH with chromogenic enzyme substrates specific for proteases and peptidases. A total of 7 antigenically distinct enzymes with peptidolytic properti...

journal_title：Molecular and biochemical parasitology

authors： Doenhoff MJ,Modha J,Curtis RH,Adeoye GO

更新日期：1988-12-01 00:00:00

abstract：:The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

journal_title：Molecular and biochemical parasitology

authors： Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

更新日期：1988-10-01 00:00:00

abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

journal_title：Molecular and biochemical parasitology

authors： Krüger T,Sanchez CP,Lanzer M

更新日期：2010-08-01 00:00:00

abstract：:Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

journal_title：Molecular and biochemical parasitology

authors： Chiba Y,Makiuchi T,Jeelani G,Nozaki T

更新日期：2016-06-01 00:00:00

abstract：:Plasmodium falciparum trophozoites were isolated by mechanical rupture of infected human erythrocytes followed by a series of differential centrifugation steps. After lysis with sonication, the 100 000 x g supernatant of parasites and uninfected host cells was used to determine the specific activities of a number of e...

journal_title：Molecular and biochemical parasitology

authors： Reyes P,Rathod PK,Sanchez DJ,Mrema JE,Rieckmann KH,Heidrich HG

更新日期：1982-05-01 00:00:00

abstract：:In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

journal_title：Molecular and biochemical parasitology

authors： Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

更新日期：2017-04-01 00:00:00

abstract：:Following the demonstration that the transport of melaminophenyl arsenical drugs in Trypanosoma brucei is dependent upon an unusual adenosine nucleoside transporter (Carter and Fairlamb, Nature 361 (1993) 173-175) we have investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a ...

journal_title：Molecular and biochemical parasitology

authors： Barrett MP,Zhang ZQ,Denise H,Giroud C,Baltz T

更新日期：1995-07-01 00:00:00

abstract：:Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

journal_title：Molecular and biochemical parasitology

authors： McIntosh MT,Srivastava R,Vaidya AB

更新日期：1998-09-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

journal_title：Molecular and biochemical parasitology

authors： Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

更新日期：1990-02-01 00:00:00

abstract：:A gene encoding the small subunit rRNA (SSUrRNA) has been isolated from the human parasite, Plasmodium malariae. The gene has been sequenced. It contains conserved and variable regions which conform to patterns established for other eukaryotic SSUrRNA genes. Comparisons with other SSUrRNA genes from Plasmodium species...

journal_title：Molecular and biochemical parasitology

authors： Goman M,Mons B,Scaife J

更新日期：1991-04-01 00:00:00

abstract：:Two dimensional electrophoresis has been used to examine protein variation in clones of two Plasmodium falciparum isolates. Variant forms of 12 proteins were detected. Five genetically distinct parasite types were identified in one isolate, and two in the second isolate. Examination of uncloned parasites using this te...

journal_title：Molecular and biochemical parasitology

authors： Fenton B,Walker A,Walliker D

更新日期：1985-08-01 00:00:00

abstract：:The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties...

journal_title：Molecular and biochemical parasitology

authors： Wan KL,Carruthers VB,Sibley LD,Ajioka JW

更新日期：1997-02-01 00:00:00

abstract：:Theileria annulata is a tick-transmitted protozoan that causes tropical theileriosis, an often fatal leukoproliferative disorder of cattle. To characterize and identify parasite proteins suitable as diagnostic antigens and/or vaccine candidates, a cDNA clone encoding a macroschizont stage protein was isolated and char...

journal_title：Molecular and biochemical parasitology

authors： Schnittger L,Katzer F,Biermann R,Shayan P,Boguslawski K,McKellar S,Beyer D,Shiels BR,Ahmed JS

更新日期：2002-04-09 00:00:00

abstract：:Hco-UNC-49 is a GABA receptor from the parasitic nematode Haemonchus contortus that has a relatively low overall sequence similarity to vertebrate GABA receptors but is very similar to the UNC-49 receptor found in the free living nematode Caenorhabditis elegans. While the nematode receptors do share >80% sequence simi...

journal_title：Molecular and biochemical parasitology

authors： Accardi MV,Forrester SG

更新日期：2011-07-01 00:00:00

abstract：:We have generated 2771 expressed sequence tags (ESTs) from two cDNA libraries of Trypanosoma cruzi CL-Brener. The libraries were constructed from trypomastigote and amastigotes, using a spliced leader primer to synthesize the cDNA second strand, thus selecting for full-length cDNAs. Since the libraries were not normal...

journal_title：Molecular and biochemical parasitology

authors： Agüero F,Abdellah KB,Tekiel V,Sánchez DO,González A

更新日期：2004-08-01 00:00:00

abstract：:Giardia lamblia depends on glycolysis to obtain ATP, highlighting the suitability of glycolytic enzymes as targets for drug design. We studied triosephosphate isomerase from G. lamblia (GlTIM) as a potential species-specific drug target. Cysteine-reactive agents were used as probes, in order to test those regions near...

journal_title：Molecular and biochemical parasitology

authors： Enriquez-Flores S,Rodriguez-Romero A,Hernandez-Alcantara G,De la Mora-De la Mora I,Gutierrez-Castrellon P,Carvajal K,Lopez-Velazquez G,Reyes-Vivas H

更新日期：2008-02-01 00:00:00

abstract：:Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lyso...

journal_title：Molecular and biochemical parasitology

authors： Vanácová S,Tachezy J,Ullu E,Tschudi C

更新日期：2001-07-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

journal_title：Molecular and biochemical parasitology

authors： Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

更新日期：2016-05-01 00:00:00

abstract：:The ATP-binding cassette (ABC) transporters are one of the largest evolutionarily conserved families of proteins. They are characterized by the presence of nucleotide-binding domains (NBDs), which are highly conserved among organisms. In the present study, we used human and protozoan ABC sequences, and ATP-binding con...

journal_title：Molecular and biochemical parasitology

authors： Sauvage V,Millot JM,Aubert D,Visneux V,Marle-Plistat M,Pinon JM,Villena I

更新日期：2006-06-01 00:00:00

abstract：:We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

journal_title：Molecular and biochemical parasitology

authors： Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

更新日期：1992-09-01 00:00:00

abstract：:Laminin is a major constituent of the basal lamina surrounding the midgut of the malaria vectors that has been implicated in the development of the Plasmodium oocyst. In this report we describe the cloning of the Anopheles gambiae gene encoding the laminin gamma 1 polypeptide and follow its expression during mosquito ...

journal_title：Molecular and biochemical parasitology

authors： Vlachou D,Lycett G,Sidén-Kiamos I,Blass C,Sinden RE,Louis C

更新日期：2001-02-01 00:00:00