Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense.


:The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of Trypanosoma (Nannomonas) congolense. The organization of the genes within the complex is similar to that of Trypanosoma brucei brucei. The nucleotide and amino-acid sequences, including those of the novel high-molecular-weight 56PGK, show substantial cross-species similarity. However, the two downstream genes, c1PGK and c2PGK, encode identical isozymes in T. congolense, while they encode distinct glycosomal and cytoplasmic isozymes in T. brucei. Western analysis also indicated that there are only two isozymes in T. congolense and that these are constitutively expressed. Differential digitonin solubilization of the trypanosomes indicated that 56PGK is primarily localized to the glycosome, as expected, and that c1/c2PGK is cytoplasmic. Northern analysis demonstrates that while 56PGK is constitutively expressed, c1PGK and c2PGK mRNAs are differentially expressed in the T. congolense developmental stages. This work demonstrates that T. congolense has only one PGK isozyme, 56PGK, that is predominantly localized in glycosomes.


Mol Biochem Parasitol


Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M




Has Abstract


1995-02-01 00:00:00














  • Mitochondrial genomes of Meloidogyne chitwoodi and M. incognita (Nematoda: Tylenchina): comparative analysis, gene order and phylogenetic relationships with other nematodes.

    abstract::Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respective...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Humphreys-Pereira DA,Elling AA

    更新日期:2014-03-01 00:00:00

  • Isolation of lambda amp3 genomic recombinants coding for antigens of Eimeria tenella.

    abstract::Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vacci...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Clarke LE,Messer LI,Greenwood NM,Wisher MH

    更新日期:1987-01-02 00:00:00

  • Protection by phospholipids of Schistosoma mansoni schistosomula against the action of cytotoxic antibodies and complement.

    abstract::Schistosoma mansoni schistosomula cultured in the presence of phospholipids showed a decreased sensitivity to the lethal complement-mediated action of anti-schistosome antibodies. Phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine had a protective action on the schistosomula transformed in vitro by pass...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Billecocq A

    更新日期:1987-09-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Electrophoretic analyses of myofibrillar proteins from the body wall muscles of Ascaris suum.

    abstract::A myofibrillar protein extract has been isolated from the muscle of Ascaris suum. Two-dimensional electrophoresis of this extract revealed that the myosin light chain 1 (ALC1) migrates as 3 components with approximate isoelectric points in the range of 5.3-5.6. The most acidic component of ALC1 appeared to be phosphor...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Srihari T,Wiehrer W,Pette D,Harris BG

    更新日期:1981-06-01 00:00:00

  • Enhanced Ca2+ uptake by mouse erythrocytes in malarial (Plasmodium berghei) infection.

    abstract::Erythrocytes from Plasmodium berghei-infected mice on incubation either in plasma or artificial isotonic media showed an increase in uptake of 45Ca2+ compared with erythrocytes from uninfected mice. Infected cells (55% parasitaemia) incubated in plasma from normal or infected mice gave uptake rates of 9.8 and 8.1 nmol...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Krungkrai J,Yuthavong Y

    更新日期:1983-03-01 00:00:00

  • A change in the premitotic period of the cell cycle is associated with bradyzoite differentiation in Toxoplasma gondii.

    abstract::We have demonstrated that bradyzoites return to the tissue-cyst stage by a developmental pathway that is indistinguishable from that initiated by sporozoites. Mature bradyzoites, like sporozoites from oocysts, were non-proliferative as they contained uniform 1N DNA contents, and replication occurred only in parasites ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Radke JR,Guerini MN,Jerome M,White MW

    更新日期:2003-10-01 00:00:00

  • Regions in the 3' untranslated region confer stage-specific expression to the Leishmania mexicana a600-4 gene.

    abstract::Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Murray A,Fu C,Habibi G,McMaster WR

    更新日期:2007-06-01 00:00:00

  • Structural and functional identification of GP57/51 antigen of Trypanosoma cruzi as a cysteine proteinase.

    abstract::Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

    更新日期:1990-11-01 00:00:00

  • An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases.

    abstract::We have determined the sequence of a cDNA (Tt34c1) encoding a Trypanosoma cruzi trypomastigote stage-specific 85-kDa surface glycoprotein (gp85). Within the peptide sequence of Tt34c1 are two 8-amino acid motifs, Ser-X-Asp-X-Gly-X-Thr-Trp, that are characteristic of bacterial neuraminidases. Analysis of the Tt34c1 seq...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Takle GB,Cross GA

    更新日期:1991-10-01 00:00:00

  • Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum.

    abstract::Leishmania parasites cycle between the alimentary tract of a sandfly vector as free-living promastigotes and the acidified phagolysosomes of the vertebrate host macrophage as aflagellated amastigotes. The differentiation process can be mimicked in host-free culture by switching promastigotes (e.g. 25 degrees C, neutra...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rochette A,Raymond F,Corbeil J,Ouellette M,Papadopoulou B

    更新日期:2009-05-01 00:00:00

  • Cloning and functional identification of a novel annexin subfamily in Cysticercus cellulosae.

    abstract::A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hongli Y,Shuhan S,Ruiwen C,Yingjun G

    更新日期:2002-01-01 00:00:00

  • Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum.

    abstract::While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bethke LL,Zilversmit M,Nielsen K,Daily J,Volkman SK,Ndiaye D,Lozovsky ER,Hartl DL,Wirth DF

    更新日期:2006-11-01 00:00:00

  • Arp1, an actin-related protein, in Plasmodium berghei.

    abstract::Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

    更新日期:2010-10-01 00:00:00

  • Presence of contaminating mitochondrial DNA from host reticulocytes in experimental infections of Plasmodium berghei.

    abstract::Superposition of two unrelated processes, namely terminal reticulocyte differentiation and synchronous plasmodial development, takes place in experimental infections of Plasmodium berghei. The first process is shown to be responsible for the appearance of some discrete restriction bands of host origin when DNA is extr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pace T,Ponzi M,Dore E,Scotti R,Mons B

    更新日期:1989-11-01 00:00:00

  • Unusual diversity in alpha-amanitin sensitivity of RNA polymerases in trichomonads.

    abstract::Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lyso...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Vanácová S,Tachezy J,Ullu E,Tschudi C

    更新日期:2001-07-01 00:00:00

  • Susceptibility of Plasmodium falciparum cyclic AMP-dependent protein kinase and its mammalian homologue to the inhibitors.

    abstract::Cyclic AMP-dependent protein kinase (protein kinase A, PKA) is a key element in many cell signaling pathways. An essential role of Plasmodium falciparum PKA (PfPKA) activity was reported in the intraerythrocytic growth of the malaria parasite. However, molecular characterization of PfPKA using purified recombinant pro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sudo A,Kato K,Kobayashi K,Tohya Y,Akashi H

    更新日期:2008-08-01 00:00:00

  • Molecular characterisation and localisation of an Onchocerca volvulus pi-class glutathione S-transferase.

    abstract::Glutathione S-transferases (GSTs) constitute a major detoxification mechanism in helminth organisms and are regarded vaccine candidates against helminth infections. Onchocerca volvulus glutathione-binding proteins were purified from the aqueous soluble fraction of homogenised adult females by affinity chromatography o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Salinas G,Braun G,Taylor DW

    更新日期:1994-07-01 00:00:00

  • Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use.

    abstract::Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cabrera Z,Parkhouse RM

    更新日期:1986-09-01 00:00:00

  • Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum.

    abstract::We have purified and characterized the Ascaris suum gamma-glutamylcysteine synthetase, the rate-limiting step in the glutathione biosynthesis. The purified enzyme exhibited a specific activity of 18 U (mg protein)-1. Estimation of the molecular mass of the native enzyme by FPLC on Superdex S-200 revealed the presence ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1995-06-01 00:00:00

  • Identification of a domain of Trypanosoma cruzi metacyclic trypomastigote surface molecule gp82 required for attachment and invasion of mammalian cells.

    abstract::Recombinant proteins and synthetic peptides representing various sequences of gp82, a surface glycoprotein of Trypanosoma cruzi metacyclic trypomastigotes implicated in mammalian cell invasion, were used in this study aiming at the identification of the domain(s) of this molecule required for interaction with target c...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Santori FR,Dorta ML,Juliano L,Juliano MA,da Silveira JF,Ruiz RC,Yoshida N

    更新日期:1996-06-01 00:00:00

  • The FAR proteins of filarial nematodes: secretion, glycosylation and lipid binding characteristics.

    abstract::The FAR proteins of nematodes are small ( approximately 20 kDa), helix-rich, fatty acid and retinol-binding (FAR) proteins that appear to be confined to nematodes. We have carried out a comparative sequence and biochemical analysis of selected FAR proteins often species of filarial parasites (from the genera Onchocerc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Garofalo A,Kläger SL,Rowlinson MC,Nirmalan N,Klion A,Allen JE,Kennedy MW,Bradley JE

    更新日期:2002-07-01 00:00:00

  • Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis.

    abstract::Leucine aminopeptidases (LAP; EC are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

    更新日期:2012-03-01 00:00:00

  • Characterization of a Cryptosporidium parvum protein that binds single-stranded G-strand telomeric DNA.

    abstract::We have initiated a project to characterize telomere-associated proteins of Cryptosporidium parvum. Searching public databases with C. parvum expressed sequence tag (EST) sequences revealed one EST sequence that is highly similar to Gbp1p of Chlamydomonas reinhardtii (Cr Gbp1p), a protein that binds single-stranded te...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liu C,Wang L,Lancto CA,Abrahamsen MS

    更新日期:2009-06-01 00:00:00

  • A kinetoplast DNA probe diagnostic for Leishmania major: sequence homologies between regions of Leishmania minicircles.

    abstract::A restriction fragment from a cloned kinetoplast minicircle DNA has been shown to be diagnostic for Leishmania major. This 402-bp TaqI fragment has been used routinely (as a radiolabelled probe) to detect 10(4) parasites in simple dot blots, both experimentally and in epidemiological surveys. It positively identified ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Smith DF,Searle S,Ready PD,Gramiccia M,Ben-Ismail R

    更新日期:1989-12-01 00:00:00

  • Microsatellite polymorphism in the sexually transmitted human pathogen Trichomonas vaginalis indicates a genetically diverse parasite.

    abstract::Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

    更新日期:2011-01-01 00:00:00

  • The fate of ferriprotorphyrin IX in malaria infected erythrocytes in conjunction with the mode of action of antimalarial drugs.

    abstract::The intraerythrocytic malaria parasite digests considerable amounts of its host cell cytosol, which consists mostly of hemoglobin. In order to avert the toxicity of ferriprotorphyrin IX (FP) thus produced, it is generally accepted that FP is polymerized to the non-toxic hemozoin. Investigating the fate of FP in cultur...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhang J,Krugliak M,Ginsburg H

    更新日期:1999-03-15 00:00:00

  • Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex.

    abstract::A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Webster P,Mansour TE,Bieber D

    更新日期:1989-10-01 00:00:00

  • Genomic organization, chromosomal location and transcription of dispersed and repeated tubulin genes in Leishmania major.

    abstract::The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Spithill TW,Samaras N

    更新日期:1987-05-01 00:00:00

  • Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.

    abstract::The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cameron S,Fyffe SA,Goldie S,Hunter WN

    更新日期:2008-04-01 00:00:00