Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex.

Abstract:

:A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-specific, repeated DNA fragments. Using W1 as a probe, we have developed a rapid and accurate dot-blot assay for determining the sex of S. mansoni cercariae.

journal_name

Mol Biochem Parasitol

authors

Webster P,Mansour TE,Bieber D

doi

10.1016/0166-6851(89)90169-2

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

217-22

issue

3

eissn

0166-6851

issn

1872-9428

pii

0166-6851(89)90169-2

journal_volume

36

pub_type

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