Interleukin-8-like activity in a filarial asparaginyl-tRNA synthetase.

abstract：:The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

journal_title：Molecular and biochemical parasitology

authors： Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

更新日期：1995-02-01 00:00:00

abstract：:The separation by chromatofocusing of two distinct purine nucleoside cleaving activities from crude extracts of Trypanosoma brucei brucei is described. One catalyzes the reversible phosphorolysis of 5'-deoxy-5'-methylthioadenosine (MeSAdo) and adenosine (Ado) and was designated an MeSAdo/Ado phosphorylase, while the o...

journal_title：Molecular and biochemical parasitology

authors： Ghoda LY,Savarese TM,Northup CH,Parks RE Jr,Garofalo J,Katz L,Ellenbogen BB,Bacchi CJ

更新日期：1988-01-15 00:00:00

abstract：:Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

journal_title：Molecular and biochemical parasitology

authors： Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

更新日期：2005-01-01 00:00:00

abstract：:This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein con...

journal_title：Molecular and biochemical parasitology

authors： Atkinson BG,Podesta RB

更新日期：1982-07-01 00:00:00

abstract：:Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

journal_title：Molecular and biochemical parasitology

authors： Pampori NA,Singh G,Srivastava VM

更新日期：1984-04-01 00:00:00

abstract：:Phosphatidylcholine is the most abundant phospholipid in the membranes of the human parasite Leishmania. The metabolic pathways leading to its biosynthesis are likely to play a critical role in parasite development and survival and may offer a good target for antileishmanial chemotherapy. Phosphatidylcholine synthesis...

journal_title：Molecular and biochemical parasitology

authors： Zufferey R,Mamoun CB

更新日期：2002-11-01 00:00:00

abstract：:A method for a 50-60-fold purification of a cysteine proteinase from trophozoites of Entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on Sephadex G-75, and preparative isoelectric focusing is described. The enzyme was examined for its proteolytic potencies towards native enzyme su...

journal_title：Molecular and biochemical parasitology

authors： Scholze H,Werries E

更新日期：1986-01-01 00:00:00

abstract：:We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

journal_title：Molecular and biochemical parasitology

authors： Toye P,Remold H

更新日期：1989-06-01 00:00:00

abstract：:A Fasciola hepatica cDNA clone of 1752 bp was isolated from an adult worm cDNA expression library by immunological screening using a rabbit serum against the excretory-secretory antigens. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 489 codons which encoded a 55 kDa polypeptide...

journal_title：Molecular and biochemical parasitology

authors： Salazar-Calderón M,Martín-Alonso JM,Castro AM,Parra F

更新日期：2003-01-01 00:00:00

abstract：:Erythrocyte invasion by malaria parasites is a multi-step process requiring specific molecular interactions between merozoites and erythrocyte surface receptors. Human Duffy blood group protein is the receptor for Plasmodium vivax merozoite invasion to red blood cells. The cognate parasite ligand for Duffy protein is ...

journal_title：Molecular and biochemical parasitology

authors： Xu L,Niu J,Chaudhuri A

更新日期：2006-04-01 00:00:00

abstract：:The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

journal_title：Molecular and biochemical parasitology

authors： Price HP,Stark M,Smith B,Smith DF

更新日期：2007-10-01 00:00:00

abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

journal_title：Molecular and biochemical parasitology

authors： Krüger T,Sanchez CP,Lanzer M

更新日期：2010-08-01 00:00:00

abstract：:Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respective...

journal_title：Molecular and biochemical parasitology

authors： Humphreys-Pereira DA,Elling AA

更新日期：2014-03-01 00:00:00

abstract：:The binding kinetics of several benzimidazole compounds were determined with recombinant tubulin from benzimidazole-sensitive and -insensitive organisms. This study utilised the naturally occurring high efficacy of the benzimidazoles for the parasitic protozoa Giardia duodenalis and Encephalitozoon intestinalis, and l...

journal_title：Molecular and biochemical parasitology

authors： MacDonald LM,Armson A,Thompson AR,Reynoldson JA

更新日期：2004-11-01 00:00:00

abstract：:We have initiated a project to characterize telomere-associated proteins of Cryptosporidium parvum. Searching public databases with C. parvum expressed sequence tag (EST) sequences revealed one EST sequence that is highly similar to Gbp1p of Chlamydomonas reinhardtii (Cr Gbp1p), a protein that binds single-stranded te...

journal_title：Molecular and biochemical parasitology

authors： Liu C,Wang L,Lancto CA,Abrahamsen MS

更新日期：2009-06-01 00:00:00

abstract：:The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

journal_title：Molecular and biochemical parasitology

authors： Dore E,Pace T,Ponzi M,Scotti R,Frontali C

更新日期：1986-11-01 00:00:00

abstract：:It was found that the digenetic trypanosomatid Endotrypanum schaudinni transferred Man7GlcNAc2 in protein N-glycosylation. Endo-beta-N-acetylglucosaminidase H-sensitive oligosaccharides were identified as Man7GlcNAc2, Man6GlcNAc2, Rib1Man6GlcNAc2 and/or Gal1Man6GlcNAc2, Man5GlcNAc containing two galactose or ribose un...

journal_title：Molecular and biochemical parasitology

authors： Merello S,Xavier MT,Parodi AJ

更新日期：1995-01-01 00:00:00

abstract：:Cyclic AMP-dependent protein kinase (protein kinase A, PKA) is a key element in many cell signaling pathways. An essential role of Plasmodium falciparum PKA (PfPKA) activity was reported in the intraerythrocytic growth of the malaria parasite. However, molecular characterization of PfPKA using purified recombinant pro...

journal_title：Molecular and biochemical parasitology

authors： Sudo A,Kato K,Kobayashi K,Tohya Y,Akashi H

更新日期：2008-08-01 00:00:00

abstract：:Following the demonstration that the transport of melaminophenyl arsenical drugs in Trypanosoma brucei is dependent upon an unusual adenosine nucleoside transporter (Carter and Fairlamb, Nature 361 (1993) 173-175) we have investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a ...

journal_title：Molecular and biochemical parasitology

authors： Barrett MP,Zhang ZQ,Denise H,Giroud C,Baltz T

更新日期：1995-07-01 00:00:00

abstract：:Erythrocyte invasion by malaria parasites requires specific receptor-ligand interactions. Plasmodium vivax and Plasmodium knowlesi are completely dependent on binding the Duffy blood group antigen to invade human erythrocytes. P. knowlesi invades rhesus erythrocytes by multiple pathways using the Duffy antigen as well...

journal_title：Molecular and biochemical parasitology

authors： Singh AP,Puri SK,Chitnis CE

更新日期：2002-04-30 00:00:00

abstract：:The reduction in hemozoin content is a well known feature of chloroquine-resistant Plasmodium berghei. Using NK65-derived lines displaying increasing resistance levels, we observed an inverse relationship between the hemozoin content, and the glutathione (GSH) and glutathione S-transferase (GST) levels. Treatment of h...

journal_title：Molecular and biochemical parasitology

authors： Platel DF,Mangou F,Tribouley-Duret J

更新日期：1999-01-25 00:00:00

abstract：:A restriction fragment from a cloned kinetoplast minicircle DNA has been shown to be diagnostic for Leishmania major. This 402-bp TaqI fragment has been used routinely (as a radiolabelled probe) to detect 10(4) parasites in simple dot blots, both experimentally and in epidemiological surveys. It positively identified ...

journal_title：Molecular and biochemical parasitology

authors： Smith DF,Searle S,Ready PD,Gramiccia M,Ben-Ismail R

更新日期：1989-12-01 00:00:00

abstract：:Intra-erythrocytic Plasmodium parasites digest host cell haemoglobin and use the liberated amino acids for protein synthesis. Although several endoproteases (aspartic, cysteine, and metallo-) have been shown to be involved in the initial stages of haemoglobin degradation, little is known about the steps immediately be...

journal_title：Molecular and biochemical parasitology

authors： Gavigan CS,Dalton JP,Bell A

更新日期：2001-09-28 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence anal...

journal_title：Molecular and biochemical parasitology

authors： Barnes DA,Bonnin A,Huang JX,Gousset L,Wu J,Gut J,Doyle P,Dubremetz JF,Ward H,Petersen C

更新日期：1998-10-30 00:00:00

abstract：:Antigenic diversity was observed in the circumsporozoite (CS) proteins of five of the six Plasmodium cynomolgi isolates (NIH, Mulligan, London, Gombak, Ceylon, RO) that we examined. Monoclonal antibodies were produced against salivary gland sporozoites of three of the isolates. Interaction of these monoclonal antibodi...

journal_title：Molecular and biochemical parasitology

authors： Cochrane AH,Gwadz RW,Ojo-Amaize E,Hii J,Nussenzweig V,Nussenzweig RS

更新日期：1985-01-01 00:00:00

abstract：:Six discrete phylogenetic lineages were recently identified in Trypanosoma cruzi, on the basis of multilocus enzyme electrophoresis and random amplified polymorphic DNA (RAPD) characterisation. The objective of the present study was to develop specific PCR-based markers for the identification of each of the six lineag...

journal_title：Molecular and biochemical parasitology

authors： Brisse S,Dujardin JC,Tibayrenc M

更新日期：2000-11-01 00:00:00

abstract：:We have compared a total of 44 recognition sites for 12 restriction endonucleases on the 20 kilobase pair maxi-circle of kinetoplast DNA from nine Trypanosoma brucei stocks, four which are known to be infective to man (tow 'gambiense' and two 'rhodesiense' variants). In addition to five polymorphic sites, these DNAs d...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Fase-Fowler F,Gibson WC

更新日期：1981-06-01 00:00:00

abstract：:The physiological role of Schistosomiasis mansoni 22.6 antigen (sm22.6 Ag) and its pathogenic effect on the human host has never been reported. Recombinant sm22.6 Ag is a homogenous polymer under non-denaturing/non-reducing conditions, and an inhibitor to human thrombin. Kinetic and Western blot assays show that the r...

journal_title：Molecular and biochemical parasitology

authors： Lin YL,He S

更新日期：2006-05-01 00:00:00

abstract：:The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

journal_title：Molecular and biochemical parasitology

authors： Cunha VM,Meyer-Fernandes JR,Noël F

更新日期：1992-06-01 00:00:00

abstract：:Two short DNA segments were isolated by screening of a lambda gt11 library from Plasmodium falciparum schizont cDNA with an antiserum against the 140 kDa protein, which confers protective immunity to monkeys. The segments were used to identify a genomic fragment which carries the entire coding sequence for a protein o...

journal_title：Molecular and biochemical parasitology

authors： Knapp B,Hundt E,Nau U,Küpper HA

更新日期：1989-01-01 00:00:00