Serological cross-reaction between high molecular weight proteins synthesized in blood schizonts of Plasmodium yoelii, Plasmodium chabaudi and Plasmodium falciparum.

abstract：:Six discrete phylogenetic lineages were recently identified in Trypanosoma cruzi, on the basis of multilocus enzyme electrophoresis and random amplified polymorphic DNA (RAPD) characterisation. The objective of the present study was to develop specific PCR-based markers for the identification of each of the six lineag...

journal_title：Molecular and biochemical parasitology

authors： Brisse S,Dujardin JC,Tibayrenc M

更新日期：2000-11-01 00:00:00

abstract：:Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

journal_title：Molecular and biochemical parasitology

authors： Campos MC,Leon LL,Taylor MC,Kelly JM

更新日期：2014-01-01 00:00:00

abstract：:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measur...

journal_title：Molecular and biochemical parasitology

authors： Walsh MJ,Blum JJ

更新日期：1992-02-01 00:00:00

abstract：:Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified...

journal_title：Molecular and biochemical parasitology

authors： Mendis AH,Rose ME,Rees HH,Goodwin TW

更新日期：1983-11-01 00:00:00

abstract：:Erythrocytes from Plasmodium berghei-infected mice on incubation either in plasma or artificial isotonic media showed an increase in uptake of 45Ca2+ compared with erythrocytes from uninfected mice. Infected cells (55% parasitaemia) incubated in plasma from normal or infected mice gave uptake rates of 9.8 and 8.1 nmol...

journal_title：Molecular and biochemical parasitology

authors： Krungkrai J,Yuthavong Y

更新日期：1983-03-01 00:00:00

abstract：:Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

journal_title：Molecular and biochemical parasitology

authors： Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

更新日期：2010-11-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00

abstract：:Studies measuring the glycolytic intermediate and adenine nucleotide concentrations in Trypanosoma brucei metabolising glucose either aerobically or under conditions where glycerol-3-phosphate oxidase is inactive have shown the following: 1. Inhibition with 0.5 mM salicylhydroxamic acid (SHAM) accurately simulates ana...

journal_title：Molecular and biochemical parasitology

authors： Hammond DJ,Bowman IB

更新日期：1980-12-01 00:00:00

abstract：:Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

journal_title：Molecular and biochemical parasitology

authors： Gao F,Voncken F,Colasante C

更新日期：2020-05-01 00:00:00

abstract：:Antigenic switching in Trypanosoma brucei can occur either by the production of a telomeric copy of a variant surface glycoprotein (VSG) gene through a gene conversion mechanism or by the nonduplicative activation of a telomeric VSG gene. The 5 VSG gene telomeric copy that is expressed in IsTaR 1 variant antigenic typ...

journal_title：Molecular and biochemical parasitology

authors： Aline RF Jr,Stuart K

更新日期：1985-06-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00

abstract：:We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits allelic variation. One lo...

journal_title：Molecular and biochemical parasitology

authors： Rangarajan D,Harvey TI,Barry JD

更新日期：2000-02-05 00:00:00

abstract：:Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the he...

journal_title：Molecular and biochemical parasitology

authors： Ntambi JM,Marini JC,Bangs JD,Hajduk SL,Jimenez HE,Kitchin PA,Klein VA,Ryan KA,Englund PT

更新日期：1984-07-01 00:00:00

abstract：:The apicomplexa parasite Toxoplasma gondii expresses two distinct proliferating cell nuclear antigens (PCNA) that exhibit distinct patterns of subcellular localization during tachyzoite growth. In all cell cycle phases, TgPCNA1 is concentrated in the nucleus, while TgPCNA2 is only concentrated in the nucleus during S-...

journal_title：Molecular and biochemical parasitology

authors： Guerini MN,Behnke MS,White MW

更新日期：2005-07-01 00:00:00

abstract：:The interaction of Leishmania promastigote surface protease (PSP) with the plasmatic protease inhibitor alpha 2-macroglobulin (alpha 2M) was investigated. In plasma, solubilized PSP forms covalent complexes only with alpha 2M, at the exclusion of other protease inhibitors. The formation of complexes is accompanied by ...

journal_title：Molecular and biochemical parasitology

authors： Heumann D,Burger D,Vischer T,de Colmenares M,Bouvier J,Bordier C

更新日期：1989-02-01 00:00:00

abstract：:The merozoite surface protein-1 of the human malaria parasite Plasmodium falciparum undergoes an extracellular proteolytic cleavage (secondary processing) intrinsic to successful erythrocyte invasion. In the T9/96 clone of P. falciparum the protease responsible has been characterised as a membrane-associated, calcium-...

journal_title：Molecular and biochemical parasitology

authors： Blackman MJ,Chappel JA,Shai S,Holder AA

更新日期：1993-11-01 00:00:00

abstract：:Sj23, the 23-kDa target antigen in Schistosoma japonicum adult worms of the hybridoma monoclonal antibody (mAb) I-134, has been identified and cloned from cDNA libraries, mAb I-134 has been successfully used in immunodiagnostic assays to detect S. japonicum infection in Philippine patients. Sequence analysis has shown...

journal_title：Molecular and biochemical parasitology

authors： Davern KM,Wright MD,Herrmann VR,Mitchell GF

更新日期：1991-09-01 00:00:00

abstract：:The major surface antigen (30 kDa) of Brugia pahangi has been characterised by a number of biochemical and immunochemical means. The 30 kDa polypeptide is a glycoprotein which can be extracted from the worm surface by homogenization in the absence of detergents. The 30 kDa polypeptide can be metabolically labelled wit...

journal_title：Molecular and biochemical parasitology

authors： Devaney E

更新日期：1988-01-01 00:00:00

abstract：:Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

journal_title：Molecular and biochemical parasitology

authors： Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

更新日期：2015-08-01 00:00:00

abstract：:A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In the parasitized erythrocyte, PfCG2 expression rises sharply in the trophozoite stage and is detected in electron-dense patches along the parasitophorous vacuolar membrane (PVM), in the cyto...

journal_title：Molecular and biochemical parasitology

authors： Cooper RA,Papakrivos J,Lane KD,Fujioka H,Lingelbach K,Wellems TE

更新日期：2005-12-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fa...

journal_title：Molecular and biochemical parasitology

authors： Dame JB,Yowell CA,Courtney CH,Lindgren WG

更新日期：1991-04-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:A convenient method was employed for the efficient extraction of total RNA from Plasmodium falciparum. By depleting the total RNA of tRNA, it was shown that P. falciparum or P. lophurae tRNAs markedly stimulate the translation of P. falciparum mRNA in the rabbit reticulocyte cell-free system. Analysis of the cell-free...

journal_title：Molecular and biochemical parasitology

authors： Wallach M

更新日期：1982-12-01 00:00:00

abstract：:The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

journal_title：Molecular and biochemical parasitology

authors： Cunha VM,Meyer-Fernandes JR,Noël F

更新日期：1992-06-01 00:00:00

abstract：:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

journal_title：Molecular and biochemical parasitology

authors： Thompson SN,Platzer EG,Lee RW

更新日期：1987-01-02 00:00:00

abstract：:The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

journal_title：Molecular and biochemical parasitology

authors： MacLeod A,Turner CM,Tait A

更新日期：1999-08-20 00:00:00

abstract：:Proteins in malaria parasites (Plasmodium falciparum) isolated from a patient in Thailand before treatment, and after recrudescence of infection subsequent to mefloquine treatment, were compared by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis. Nine 'pre-treatment' and six 'recrudescent' clones...

journal_title：Molecular and biochemical parasitology

authors： Pinswasdi C,Thaithong S,Beale GH,Fenton B,Webster HK,Pavanand K

更新日期：1987-03-01 00:00:00

abstract：:Genetic diversity within and between tandemly arrayed copies of the Gp63 gene occurs in laboratory isolates of Leishmania spp., but the extent to which this represents natural genetic diversity has not been assessed. Here, the Gp63 locus is examined in 58 fresh isolates of L. peruviana, and clones derived from them, c...

journal_title：Molecular and biochemical parasitology

authors： Espinoza JR,Skinner AC,Davies CR,Llanos-Cuentas A,Arevalo J,Dye C,McMaster WR,Ajioka JW,Blackwell JM

更新日期：1995-06-01 00:00:00

abstract：:Ribosomal RNA isolated from the malaria parasite Plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 X 10(6) and 0.78 +/- 0.02 X 10(6) present in equimolar quantities. Their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. T...

journal_title：Molecular and biochemical parasitology

authors： Hyde JE,Zolg JW,Scaife JG

更新日期：1981-12-31 00:00:00