Susceptibility of Plasmodium falciparum cyclic AMP-dependent protein kinase and its mammalian homologue to the inhibitors.

abstract：:A 230 000 molecular weight (MW) Plasmodium yoelii protein, a 250 000 MW P. chabaudi protein and a 195 000 MW P. falciparum protein, identified using monoclonal antibodies, have similar characteristics, and have been implicated as protective antigens. In this study the serological relationship between these proteins wa...

journal_title：Molecular and biochemical parasitology

authors： Holder AA,Freeman RR,Newbold CI

更新日期：1983-11-01 00:00:00

abstract：:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

journal_title：Molecular and biochemical parasitology

authors： Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

更新日期：2020-07-01 00:00:00

abstract：:Laminin is a major constituent of the basal lamina surrounding the midgut of the malaria vectors that has been implicated in the development of the Plasmodium oocyst. In this report we describe the cloning of the Anopheles gambiae gene encoding the laminin gamma 1 polypeptide and follow its expression during mosquito ...

journal_title：Molecular and biochemical parasitology

authors： Vlachou D,Lycett G,Sidén-Kiamos I,Blass C,Sinden RE,Louis C

更新日期：2001-02-01 00:00:00

abstract：:The sequence of two fragments derived from the variable region of the kinetoplast maxicircle of Trypanosoma brucei has been determined. One fragment (1334 nucleotides, situated immediately upstream of the 12S and 9S ribosomal RNA genes) consists of non-repetitious DNA, which does not hybridize to other maxicircle regi...

journal_title：Molecular and biochemical parasitology

authors： de Vries BF,Mulder E,Brakenhoff JP,Sloof P,Benne R

更新日期：1988-01-01 00:00:00

abstract：:Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

journal_title：Molecular and biochemical parasitology

authors： Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

更新日期：2015-08-01 00:00:00

abstract：:The glycosylphosphatidylinositol phospholipase C (GPI-PLC) from Trypanosoma brucei is particularly effective in hydrolysing the GPI-anchors of some proteins. The enzyme is inhibited by Zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be...

journal_title：Molecular and biochemical parasitology

authors： Carnall N,Webb H,Carrington M

更新日期：1997-12-15 00:00:00

abstract：:We have demonstrated previously that Nopp44/46, an abundant nucleolar phosphoprotein of Trypanosoma brucei, is associated with a protein kinase. In many organisms multiple nucleolar proteins are phosphorylated by the protein kinase CK2, formerly known as casein kinase II. Here we report the identification of two T. br...

journal_title：Molecular and biochemical parasitology

authors： Park JH,Brekken DL,Randall AC,Parsons M

更新日期：2002-01-01 00:00:00

abstract：:The interaction of Leishmania promastigote surface protease (PSP) with the plasmatic protease inhibitor alpha 2-macroglobulin (alpha 2M) was investigated. In plasma, solubilized PSP forms covalent complexes only with alpha 2M, at the exclusion of other protease inhibitors. The formation of complexes is accompanied by ...

journal_title：Molecular and biochemical parasitology

authors： Heumann D,Burger D,Vischer T,de Colmenares M,Bouvier J,Bordier C

更新日期：1989-02-01 00:00:00

abstract：:Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96,000, 80,000 and 50,000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of po...

journal_title：Molecular and biochemical parasitology

authors： Symons FM,Murray PJ,Ji H,Simpson RJ,Osborn AH,Cappai R,Handman E

更新日期：1994-09-01 00:00:00

abstract：:The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation ...

journal_title：Molecular and biochemical parasitology

authors： Ramos F,García G,Valadez A,Morán P,González E,Gómez A,Melendro EI,Valenzuela O,Ximénez C

更新日期：2005-06-01 00:00:00

abstract：:The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

journal_title：Molecular and biochemical parasitology

authors： Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

更新日期：2002-06-01 00:00:00

abstract：:Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

journal_title：Molecular and biochemical parasitology

authors： Cacciò SM,Ryan U

更新日期：2008-08-01 00:00:00

abstract：:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

journal_title：Molecular and biochemical parasitology

authors： el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

更新日期：1995-07-01 00:00:00

abstract：:We have determined the sequence of a cDNA (Tt34c1) encoding a Trypanosoma cruzi trypomastigote stage-specific 85-kDa surface glycoprotein (gp85). Within the peptide sequence of Tt34c1 are two 8-amino acid motifs, Ser-X-Asp-X-Gly-X-Thr-Trp, that are characteristic of bacterial neuraminidases. Analysis of the Tt34c1 seq...

journal_title：Molecular and biochemical parasitology

authors： Takle GB,Cross GA

更新日期：1991-10-01 00:00:00

abstract：:The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

journal_title：Molecular and biochemical parasitology

authors： Barral V,This P,Imbert-Establet D,Combes C,Delseny M

更新日期：1993-06-01 00:00:00

abstract：:The variant-specific surface glycoprotein (VSG) genes of Trypanosoma brucei are invariably expressed near the ends of chromosomes (telomeres). We have targeted a VSG gene expression site (ES) promoter driving a selectable marker gene (neomycin phosphotransferase) into a chromosome-internal transcription unit, the tubu...

journal_title：Molecular and biochemical parasitology

authors： Zomerdijk JC,Kieft R,Borst P

更新日期：1993-02-01 00:00:00

abstract：:The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

journal_title：Molecular and biochemical parasitology

authors： Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

更新日期：2016-01-01 00:00:00

abstract：:Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

journal_title：Molecular and biochemical parasitology

authors： Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

更新日期：2010-11-01 00:00:00

abstract：:The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host i...

journal_title：Molecular and biochemical parasitology

authors： Aragon AD,Imani RA,Blackburn VR,Cunningham C

更新日期：2008-12-01 00:00:00

abstract：:Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

journal_title：Molecular and biochemical parasitology

authors： McIntosh MT,Srivastava R,Vaidya AB

更新日期：1998-09-01 00:00:00

abstract：:Leishmania parasites cycle between the alimentary tract of a sandfly vector as free-living promastigotes and the acidified phagolysosomes of the vertebrate host macrophage as aflagellated amastigotes. The differentiation process can be mimicked in host-free culture by switching promastigotes (e.g. 25 degrees C, neutra...

journal_title：Molecular and biochemical parasitology

authors： Rochette A,Raymond F,Corbeil J,Ouellette M,Papadopoulou B

更新日期：2009-05-01 00:00:00

abstract：:The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, contains chitin, which is a homopolymer of beta-1, 4-linked N-acetyl-glucosamine (GlcNAc). In fungi and in bacteria that make nodulation factors, chitin deacetylases make chitosan, which is a mixture of GlcNAc and glucosami...

journal_title：Molecular and biochemical parasitology

authors： Das S,Van Dellen K,Bulik D,Magnelli P,Cui J,Head J,Robbins PW,Samuelson J

更新日期：2006-07-01 00:00:00

abstract：:A genomic clone, pTt21, containing DNA apparently transcribed specifically in Trypanosoma cruzi trypomastigotes, was obtained by differentially screening a genomic library with trypomastigote and epimastigote cDNA. This 3444-bp clone contained open reading frames at each end, separated by a 1.8-kb non-coding region. T...

journal_title：Molecular and biochemical parasitology

authors： Takle GB,O'Connor J,Young AJ,Cross GA

更新日期：1992-11-01 00:00:00

abstract：:The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

journal_title：Molecular and biochemical parasitology

authors： Dore E,Pace T,Ponzi M,Scotti R,Frontali C

更新日期：1986-11-01 00:00:00

abstract：:The identification and characterization of a recombinant cDNA clone, designated OV9M, expressing an antigen present in Onchocerca volvulus infective larvae and adult stages is described. Clone OV9M was identified by screening a lambda gt11 cDNA expression library derived from adult O. volvulus mRNA using pooled rabbit...

journal_title：Molecular and biochemical parasitology

authors： Irvine M,Huima T,Prince AM,Lustigman S

更新日期：1994-05-01 00:00:00

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

journal_title：Molecular and biochemical parasitology

authors： Thompson SN,Platzer EG,Lee RW

更新日期：1987-01-02 00:00:00

abstract：:The glycine cleavage complex (GCV) is a potential source of the one carbon donor 5,10-methylene-tetrahydrofolate (5,10-CH(2)-THF) in the malaria parasite Plasmodium falciparum. One carbon (C1) donor units are necessary for amino acid and nucleotide biosynthesis, and for the initiation of mitochondrial and plastid tran...

journal_title：Molecular and biochemical parasitology

authors： Spalding MD,Allary M,Gallagher JR,Prigge ST

更新日期：2010-08-01 00:00:00

abstract：:Contrary to previous reports in the literature, bloodstream forms of the haemoflagellate protozoan Trypanosoma brucei brucei are not deficient in their ability to metabolize hydrogen peroxide, although they either lack or only possess the normal enzymes for H2O2 detoxification, catalase (EC 1.11.1.6) and glutathione p...

journal_title：Molecular and biochemical parasitology

authors： Penketh PG,Klein RA

更新日期：1986-08-01 00:00:00

abstract：:Unequal usage of synonymous codons in the gene transcript for an amino acid is known as codon usage bias (CUB). It is a unique property of gene as well as genome. Mutation and natural selection are known to be the major factors that influence CUB. Other factors encompass gene expression level, GC content, codon positi...

journal_title：Molecular and biochemical parasitology

authors： Mazumder GA,Uddin A,Chakraborty S

更新日期：2018-10-01 00:00:00