Differentiation of Trypanosoma brucei bloodstream trypomastigotes from long slender to short stumpy-like forms in axenic culture.


:An axenic cultivation system was used to study the differentiation of Trypanosoma brucei bloodstream forms from long slender to short stumpy-like forms. Trypanosomes in the logarithmic phase are similar to long slender bloodstream forms freshly isolated from infected mice, differing only in the rate of oxygen uptake. In contrast, trypanosomes in the stationary phase show a decreased level of glucose oxidation, express pyrroline-5-carboxylate reductase (proline oxidase), are inhibited in oxygen uptake to about 44% by KCN, undergo considerable morphological changes on the cellular and subcellular level, and have a significantly smaller cell volume. These results are comparable to those observed during the differentiation of long slender to short stumpy forms in infected animals, suggesting that the differentiation process towards insect procyclic forms can be initiated in culture at 37 degrees C. As judged from immunofluorescence and electron microscopy analysis, the surface coat remains intact.


Mol Biochem Parasitol


Hamm B,Schindler A,Mecke D,Duszenko M




Has Abstract


1990-04-01 00:00:00














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    journal_title:Molecular and biochemical parasitology

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    authors: Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

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    journal_title:Molecular and biochemical parasitology

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    authors: Dwyer DM,Gottlieb M

    更新日期:1984-02-01 00:00:00

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    authors: Takamiya S,Furushima R,Oya H

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    authors: Ackers JP,Dhir V,Field MC

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    authors: Sandlund L,Nilsen F,Male R,Dalvin S

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gillin FD,Reiner DS,Levy RB,Henkart PA

    更新日期:1984-09-01 00:00:00

  • Characterization of antigens on mosquito midgut stages of Plasmodium gallinaceum. II. Comparison of surface antigens of male and female gametes and zygotes.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kaushal DC,Carter R

    更新日期:1984-04-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Cacciò SM,Ryan U

    更新日期:2008-08-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bishop RP

    更新日期:1990-01-01 00:00:00