Abstract:
:In trypanosomes, trans-splicing is a major essential RNA-processing mechanism that involves the addition of a spliced leader sequence to all mRNAs from a small RNA species, known as the spliced leader RNA (SL RNA). SL RNA maturation is poorly understood and it is not clear where assembly with Sm proteins takes place. In this study, we followed the localization of the SL RNA during knockdown of Sm proteins and XPO1, which in metazoa functions in transport of mRNA and U snRNAs from the nucleus to the cytoplasm. We found that XPO1 has no role in SL RNA biogenesis in wild-type cells, or when the cells are depleted of Sm proteins. During Sm depletion, 'defective' SL RNA lacking cap modification at position +4 first accumulates in the nucleus, suggesting that Sm assembly on SL RNA most probably takes place in this compartment. Only after massive nuclear accumulation is the 'defective' SL RNA exported to the cytoplasm to form SL RNP-C, which may be a route to dispose of SL RNA when its normal biogenesis is blocked.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Biton M,Mandelboim M,Arvatz G,Michaeli Sdoi
10.1016/j.molbiopara.2006.07.004subject
Has Abstractpub_date
2006-12-01 00:00:00pages
132-43issue
2eissn
0166-6851issn
1872-9428pii
S0166-6851(06)00222-2journal_volume
150pub_type
杂志文章abstract::Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...
journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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