Dominant lethality and insect population control.

abstract：:Lectins comprise a family of related proteins that mediate essential cell functions through binding to carbohydrates. Within this protein family, C-type lectins are defined by the requirement of calcium for optimal biologic activity. Using reverse transcription PCR, a cDNA corresponding to a putative C-type lectin has...

journal_title：Molecular and biochemical parasitology

authors： Brown AC,Harrison LM,Kapulkin W,Jones BF,Sinha A,Savage A,Villalon N,Cappello M

更新日期：2007-02-01 00:00:00

abstract：:Cathepsin B-like cysteine protease (cbl) genes produce the most abundant mRNAs ( approximately 16%) detected in the adult female intestine of the parasitic nematode Haemonchus contortus. CBL enzymes appear to digest host proteins and are vaccine candidates for immune control of H. contortus and potentially other paras...

journal_title：Molecular and biochemical parasitology

authors： Jasmer DP,Mitreva MD,McCarter JP

更新日期：2004-10-01 00:00:00

abstract：:Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

journal_title：Molecular and biochemical parasitology

authors： Handman E,Hocking RE,Mitchell GF,Spithill TW

更新日期：1983-02-01 00:00:00

abstract：:The Trypanosoma cruzi insect stage-specific antigen GP72 was purified from epimastigotes and the amino acid sequences of peptide fragments determined. Oligonucleotides derived from these data were used to amplify and clone a cDNA sequence, which was used to isolate a full-length gene. All the sequenced peptides were e...

journal_title：Molecular and biochemical parasitology

authors： Cooper R,Inverso JA,Espinosa M,Nogueira N,Cross GA

更新日期：1991-11-01 00:00:00

abstract：:The pyrimidine biosynthesis pathway in the protozoan pathogen Toxoplasma gondii is essential for parasite growth during infection. To investigate the properties of dihydroorotate dehydrogenase (TgDHOD), the fourth enzyme in the T. gondii pyrimidine pathway, we expressed and purified recombinant TgDHOD. TgDHOD exhibite...

journal_title：Molecular and biochemical parasitology

authors： Hortua Triana MA,Huynh MH,Garavito MF,Fox BA,Bzik DJ,Carruthers VB,Löffler M,Zimmermann BH

更新日期：2012-08-01 00:00:00

abstract：:The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated b...

journal_title：Molecular and biochemical parasitology

authors： Perry DA,Morrison HG,Adam RD

更新日期：2011-12-01 00:00:00

abstract：:Investigating the proteome of intracellular Leishmania amastigotes has recently become possible due to the exploitation of fluorescence activated intracellular parasite sorting. Here, we employed this technology in combination with gel free analysis to greatly improve proteome coverage and suggest proteins putatively ...

journal_title：Molecular and biochemical parasitology

authors： Paape D,Barrios-Llerena ME,Le Bihan T,Mackay L,Aebischer T

更新日期：2010-02-01 00:00:00

abstract：:Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced...

journal_title：Molecular and biochemical parasitology

authors： Cass CL,Johnson JR,Califf LL,Xu T,Hernandez HJ,Stadecker MJ,Yates JR 3rd,Williams DL

更新日期：2007-10-01 00:00:00

abstract：:A Trypanosoma cruzi genomic expression library was screened with a pool of sera obtained from chronic chagasic patients. The recombinant antigen (Tc40) isolated from this library reacted with a large number of serum samples of chronic chagasic patients, suggesting that the presence of anti-Tc40 antibodies may be speci...

journal_title：Molecular and biochemical parasitology

authors： Lesénéchal M,Duret L,Cano MI,Mortara RA,Jolivet M,Camargo ME,da Silveira JF,Paranhos-Baccalà G

更新日期：1997-08-01 00:00:00

abstract：:Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

journal_title：Molecular and biochemical parasitology

authors： McIntosh MT,Srivastava R,Vaidya AB

更新日期：1998-09-01 00:00:00

abstract：:Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modu...

journal_title：Molecular and biochemical parasitology

authors： Rojas-Pirela M,Rigden DJ,Michels PA,Cáceres AJ,Concepción JL,Quiñones W

更新日期：2018-01-01 00:00:00

abstract：:Helicases are ubiquitous essential enzymes which have significant role in the nucleic acid metabolism. Using in silico approaches in the recent past we have identified a number of helicases in the Plasmodium falciparum genome. In the present study we report purification and detailed characterization of a novel helicas...

journal_title：Molecular and biochemical parasitology

authors： Mehta J,Tuteja R

更新日期：2011-03-01 00:00:00

abstract：:The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

journal_title：Molecular and biochemical parasitology

authors： DaRocha WD,Otsu K,Teixeira SM,Donelson JE

更新日期：2004-02-01 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00

abstract：:Genetic transformation of malaria parasites has been limited by the number of selectable markers available. For the rodent malaria parasite, Plasmodium berghei, only a single selection marker has been at hand, utilising the dihydrofolate reductase-thymidylate synthase gene from either P. berghei or Toxoplasma gondii t...

journal_title：Molecular and biochemical parasitology

authors： de Koning-Ward TF,Fidock DA,Thathy V,Menard R,van Spaendonk RM,Waters AP,Janse CJ

更新日期：2000-03-05 00:00:00

abstract：:In trypanosomes and related kinetoplastida a common short 5' leader sequence is encoded by the highly repetitive mini-exon gene and added to all protein coding mRNAs by trans-splicing. To initiate a search for transcription factors associated with transcription of the mini-exon we have used an electrophoretic mobility...

journal_title：Molecular and biochemical parasitology

authors： Koronakis VE,Ross AM,Le Page RW,Hughes C

更新日期：1988-07-01 00:00:00

abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

journal_title：Molecular and biochemical parasitology

authors： Dissanayake S,Xu M,Piessens WF

更新日期：1992-12-01 00:00:00

abstract：:The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

journal_title：Molecular and biochemical parasitology

authors： Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

更新日期：1988-02-01 00:00:00

abstract：:Nucleotide-sugar transporters (NSTs) transport activated sugars (e.g. UDP-GlcNAc) from the cytosol to the lumen of the endoplasmic reticulum or Golgi apparatus where they are used to make glycoproteins and glycolipids. UDP-Glc is an important component of the N-glycan-dependent quality control (QC) system for protein ...

journal_title：Molecular and biochemical parasitology

authors： Banerjee S,Cui J,Robbins PW,Samuelson J

更新日期：2008-05-01 00:00:00

abstract：:Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

journal_title：Molecular and biochemical parasitology

authors： Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

更新日期：1991-07-01 00:00:00

abstract：:It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

journal_title：Molecular and biochemical parasitology

authors： Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

更新日期：1997-09-01 00:00:00

abstract：:The azole antifungals ketoconazole and itraconazole possess in vitro antileishmanial activity against Leishmania mexicana mexicana amastigotes in macrophages (cell line J774G8). As in yeast and fungi, the activity is likely to be due to inhibition of the cytochrome P-450-dependent 14 alpha-demethylation of lanosterol ...

journal_title：Molecular and biochemical parasitology

authors： Hart DT,Lauwers WJ,Willemsens G,Vanden Bossche H,Opperdoes FR

更新日期：1989-03-01 00:00:00

abstract：:Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the comp...

journal_title：Molecular and biochemical parasitology

authors： Babon JJ,Morgan WD,Kelly G,Eccleston JF,Feeney J,Holder AA

更新日期：2007-05-01 00:00:00

abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

journal_title：Molecular and biochemical parasitology

authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

更新日期：2000-12-01 00:00:00

abstract：:Leishmania major aquaglyceroporin (AQP1) is an adventitious metalloid channel that allows the bidirectional movement of arsenite and antimonite. Here we demonstrate that AQP1 is subjected to proteasome-dependent degradation. Treatment of Leishmania promastigotes with the proteasome inhibitor MG132 resulted in increase...

journal_title：Molecular and biochemical parasitology

authors： Sharma M,Mandal G,Mandal S,Bhattacharjee H,Mukhopadhyay R

更新日期：2015-06-01 00:00:00

abstract：:Tropomyosins are a family of actin-binding proteins with diverse roles in actin filament function. One of the best characterized roles is the regulation of muscle contraction. Tropomyosin isoforms can be generated from different genes, and from alternative promoters and alternative splicing from the same gene. In this...

journal_title：Molecular and biochemical parasitology

authors： Koziol U,Costábile A,Domínguez MF,Iriarte A,Alvite G,Kun A,Castillo E

更新日期：2011-02-01 00:00:00

abstract：:The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

journal_title：Molecular and biochemical parasitology

authors： Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

更新日期：2001-03-01 00:00:00

abstract：:The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

journal_title：Molecular and biochemical parasitology

authors： Coppel RL

更新日期：1992-02-01 00:00:00

abstract：:The merozoite of Plasmodium vivax possesses a high molecular mass surface protein called Pv-merozoite surface protein 1, PvMSP-1, which exhibits antigenic diversity among isolates. In this study, the extent of sequence variation in the polymorphic region and the flanking interspecies conserved blocks (ICBs) 5 and 6 of...

journal_title：Molecular and biochemical parasitology

authors： Putaporntip C,Jongwutiwes S,Tanabe K,Thaithong S

更新日期：1997-01-01 00:00:00

abstract：:The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

journal_title：Molecular and biochemical parasitology

authors： Bishop RP

更新日期：1990-01-01 00:00:00