Hepatic mixed-function oxidase activity in mice infected with Trypanosoma brucei gambiense or treated with trypanocides.

abstract：:The emergence of drug-resistant Leishmania species is a significant problem in several countries. A comparative proteomic analysis of antimony-susceptible and antimony-resistant Leishmania braziliensis (LbSbR) and Leishmania infantum chagasi (LcSbR) lines was carried out using two-dimensional gel electrophoresis (2-DE...

journal_title：Molecular and biochemical parasitology

authors： Matrangolo FS,Liarte DB,Andrade LC,de Melo MF,Andrade JM,Ferreira RF,Santiago AS,Pirovani CP,Silva-Pereira RA,Murta SM

更新日期：2013-08-01 00:00:00

abstract：:The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

journal_title：Molecular and biochemical parasitology

authors： Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

更新日期：1992-07-01 00:00:00

abstract：:Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoites store TLN4 in the micronemes and secrete it in response to elevated calcium, ...

journal_title：Molecular and biochemical parasitology

authors： Laliberté J,Carruthers VB

更新日期：2011-05-01 00:00:00

abstract：:Congopain and cruzipain, the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi, were compared for their activities towards a series of new, sensitive fluorogenic substrates of the papain family of cysteine proteinases and for their sensitivity to inhibition by cystatins and related biotinyla...

journal_title：Molecular and biochemical parasitology

authors： Chagas JR,Authie E,Serveau C,Lalmanach G,Juliano L,Gauthier F

更新日期：1997-09-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00

abstract：:Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable me...

journal_title：Molecular and biochemical parasitology

authors： Rofatto HK,Tararam CA,Borges WC,Wilson RA,Leite LC,Farias LP

更新日期：2009-07-01 00:00:00

abstract：:Entamoeba histolytica is a eukaryotic intestinal parasite of humans, and is endemic in developing countries. We have characterized the E. histolytica putative low molecular weight protein tyrosine phosphatase (LMW-PTP). The structure for this amebic tyrosine phosphatase was solved, showing the ligand-induced conformat...

journal_title：Molecular and biochemical parasitology

authors： Linford AS,Jiang NM,Edwards TE,Sherman NE,Van Voorhis WC,Stewart LJ,Myler PJ,Staker BL,Petri WA Jr

更新日期：2014-01-01 00:00:00

abstract：:Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

journal_title：Molecular and biochemical parasitology

authors： Keegan F,Blum JJ

更新日期：1990-03-01 00:00:00

abstract：:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measur...

journal_title：Molecular and biochemical parasitology

authors： Walsh MJ,Blum JJ

更新日期：1992-02-01 00:00:00

abstract：:Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

journal_title：Molecular and biochemical parasitology

authors： Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

更新日期：1990-11-01 00:00:00

abstract：:An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC 2.4.2.1). ANP is specific for adenine nucleosides...

journal_title：Molecular and biochemical parasitology

authors： Savarese TM,El Kouni MH

更新日期：2014-03-01 00:00:00

abstract：:Entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. Parasite vesicle trafficking and motility, which relies on vesicle trafficking to deliver membrane and membrane components to the leading edge, are important for viru...

journal_title：Molecular and biochemical parasitology

authors： Welter BH,Temesvari LA

更新日期：2004-06-01 00:00:00

abstract：:Schistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut...

journal_title：Molecular and biochemical parasitology

authors： Mathieson W,Castro-Borges W,Wilson RA

更新日期：2011-02-01 00:00:00

abstract：:Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recent...

journal_title：Molecular and biochemical parasitology

authors： Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

更新日期：1999-03-15 00:00:00

abstract：:A group of Leishmania mexicana cysteine proteases that differ from those previously found in this protozoon are described. The enzymes characteristically have a preference for peptidyl substrates with a phenylalanyl-valyl-arginyl moiety, do not hydrolyse gelatin in substrate-sodium dodecyl sulphate polyacrylamide gels...

journal_title：Molecular and biochemical parasitology

authors： Robertson CD,Coombs GH

更新日期：1993-12-01 00:00:00

abstract：:The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of pu...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Galatis D,Cowman AF

更新日期：1993-04-01 00:00:00

abstract：:A wide range of secondary biological functions have been documented for eukaryotic aminoacyl-tRNA synthetases including roles in transcriptional regulation, mitochondrial RNA splicing, cell growth, and chemokine-like activities. The asparaginyl-tRNA synthetase (AsnRS) of the filarial nematode, Brugia malayi, is a high...

journal_title：Molecular and biochemical parasitology

authors： Kron MA,Wang C,Vodanovic-Jankovic S,Howard OM,Kuhn LA

更新日期：2012-09-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:The spontaneous emission of chemiluminescence by Trypanosoma cruzi epimastigotes was 133 +/- 5 counts s-1 (mg protein)-1. The measured intracellular steady state concentration of hydrogen peroxide in the same cells was 1.5 +/- 0.5 microM. These two values are about 12- and 15-times higher than the corresponding ones f...

journal_title：Molecular and biochemical parasitology

authors： Giulivi C,Turrens JF,Boveris A

更新日期：1988-09-01 00:00:00

abstract：:Representatives of all major lineages of eukaryotes contain peroxisomes with similar morphology and mode of biogenesis, indicating a monophyletic origin of the organelles within the common ancestor of all eukaryotes. Peroxisomes originated from the endoplasmic reticulum, but despite a common origin and shared morpholo...

journal_title：Molecular and biochemical parasitology

authors： Gabaldón T,Ginger ML,Michels PA

更新日期：2016-01-01 00:00:00

abstract：:The presence of virus-derived RNA was investigated in 38 axenically growing Giardia isolates from different geographic areas. The RNA virus was demonstrated in Giardia strains from humans in the U.S.A., England and the majority of strains from Poland. Two strains isolated respectively from a cat and a cavia also conta...

journal_title：Molecular and biochemical parasitology

authors： De Jonckheere JF,Gordts B

更新日期：1987-02-01 00:00:00

abstract：:Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

journal_title：Molecular and biochemical parasitology

authors： Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

更新日期：2011-01-01 00:00:00

abstract：:Nucleoside analogs are potential anti-Leishmania agents. To better understand how these compounds might lose their effectiveness, Leishmania were independently selected for resistance to inosine dialdehyde or tubercidin. Each of the resistant cells exhibited resistance to inosine dialdehyde and tubercidin as well as t...

journal_title：Molecular and biochemical parasitology

authors： Kerby BR,Detke S

更新日期：1993-08-01 00:00:00

abstract：:Cystic echinococcosis in humans and livestock animals is caused by infection with the cestode parasite Echinococcus granulosus. A number of genotypes of the parasite (designated G1-G10) are known to exist, with the genotype cluster G1-G3 and genotype G6 being responsible for the majority of humans infections. A recomb...

journal_title：Molecular and biochemical parasitology

authors： Alvarez Rojas CA,Gauci CG,Nolan MJ,Harandi MF,Lightowlers MW

更新日期：2012-06-01 00:00:00

abstract：:We have demonstrated previously that Nopp44/46, an abundant nucleolar phosphoprotein of Trypanosoma brucei, is associated with a protein kinase. In many organisms multiple nucleolar proteins are phosphorylated by the protein kinase CK2, formerly known as casein kinase II. Here we report the identification of two T. br...

journal_title：Molecular and biochemical parasitology

authors： Park JH,Brekken DL,Randall AC,Parsons M

更新日期：2002-01-01 00:00:00

abstract：:The microaerophilic parasite Trichomonas vaginalis is a causative agent of painful vaginitis or urethritis, termed trichomoniasis, and can also cause preterm delivery or stillbirth. Treatment of trichomoniasis is almost exclusively based on the nitroimidazole drugs metronidazole and tinidazole. Metronidazole resistanc...

journal_title：Molecular and biochemical parasitology

authors： Leitsch D,Drinić M,Kolarich D,Duchêne M

更新日期：2012-06-01 00:00:00

abstract：:Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

journal_title：Molecular and biochemical parasitology

authors： Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

更新日期：2015-08-01 00:00:00

abstract：:While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of d...

journal_title：Molecular and biochemical parasitology

authors： Bethke LL,Zilversmit M,Nielsen K,Daily J,Volkman SK,Ndiaye D,Lozovsky ER,Hartl DL,Wirth DF

更新日期：2006-11-01 00:00:00

abstract：:The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction...

journal_title：Molecular and biochemical parasitology

authors： Flynn IW,Bowman IB

更新日期：1981-11-01 00:00:00

abstract：:Thrombospondin-related anonymous protein (TRAP) family members participate in attachment and invasion of host cells by apicomplexan parasites. A TRAP homologue in Neospora caninum strain Nc-1 (NcMIC2) was cloned, sequenced and found to be 61% identical (75% similar) at the amino acid level to Toxoplasma gondii MIC2 (T...

journal_title：Molecular and biochemical parasitology

authors： Lovett JL,Howe DK,Sibley LD

更新日期：2000-03-15 00:00:00