Molecular cloning and nuclear localization of a histone deacetylase homologue in Plasmodium falciparum.


:Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recently suggested to be the target of a fungus-derived antiprotozoal agent exhibiting structural similarity to known HDAC inhibitors. We have initiated a study of HDAC of human malaria parasite, Plasmodium falciparum, to evaluate its potential as the target for novel antimalarials and its role in parasite development. We have isolated HDAC1 gene from the P. falciparum genomic and cDNA libraries. The nucleotide sequence contains no intervening sequence and its open reading frame (ORF) codes for a protein of 449 amino acid residues. We have named the protein, PfHDAC1, as the sequence shows significant homology to yeast, human and other eukaryotic HDACs. Northern blot analysis of the total RNA from different asexual and sexual stages of the parasite reveals the presence of single mRNA transcript, which is predominantly expressed in mature asexual blood stages and in gametocytes. Antiserum raised against a carboxyl terminal peptide immunoprecipitated an in vitro translated P. falciparum HDAC gene product and recognized an approximately 50 kDa protein in the Triton X-100 insoluble fraction of parasites. Immunoelectron microscopy analysis showed majority of the protein localized in the nucleus of P. falciparum. To our knowledge, this is the first HDAC gene isolated from the malaria parasite.


Mol Biochem Parasitol


Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C




Has Abstract


1999-03-15 00:00:00














  • Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries.

    abstract::We have generated 2771 expressed sequence tags (ESTs) from two cDNA libraries of Trypanosoma cruzi CL-Brener. The libraries were constructed from trypomastigote and amastigotes, using a spliced leader primer to synthesize the cDNA second strand, thus selecting for full-length cDNAs. Since the libraries were not normal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Agüero F,Abdellah KB,Tekiel V,Sánchez DO,González A

    更新日期:2004-08-01 00:00:00

  • Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use.

    abstract::Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cabrera Z,Parkhouse RM

    更新日期:1986-09-01 00:00:00

  • Cloning and sequence analysis of the Schistosoma mansoni membrane glycoprotein antigen gene GP22.

    abstract::A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

    更新日期:1991-11-01 00:00:00

  • Identification of the Echinococcus (hydatid disease) organisms using cloned DNA markers.

    abstract::Cloned DNA fragments of the ribosomal RNA gene of Schistosoma mansoni hybridise strongly to Echinococcus DNA following restriction endonuclease and Southern transfer analysis. Individuals within a strain of E. granulosus exhibit identical patterns of hybridisation. However, the hybridisation patterns show significant ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: McManus DP,Simpson AJ

    更新日期:1985-11-01 00:00:00

  • Structure of mRNA encoded by tubulin genes in Leishmania enriettii.

    abstract::Both the alpha- and beta-tubulin genes of Leishmania enriettii are encoded by mRNA of 2.0-2.2 kb in length. We have shown previously that the alpha- and beta-tubulin genes are arranged in separate, tandem repeats of 2 and 4 kb, respectively, and now report the mapping of mature mRNA onto these cloned genes. Here we sh...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Landfear SM,Wirth DF

    更新日期:1985-04-01 00:00:00

  • Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei.

    abstract::Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

    更新日期:2006-09-01 00:00:00

  • A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable minisatellites.

    abstract::The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: MacLeod A,Turner CM,Tait A

    更新日期:1999-08-20 00:00:00

  • Differential toxicity of ricin and diphtheria toxin for bloodstream forms of Trypanosoma brucei.

    abstract::The cytotoxicity of ricin and diphtheria toxin was studied in culture-adapted bloodstream forms of Trypanosoma brucei. Although ricin is endocytosed at a rate comparable to that of other internalized macromolecules, it is nontoxic to bloodstream-form trypanosomes. The resistance lies partly in low susceptibility of th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scory S,Steverding D

    更新日期:1997-12-01 00:00:00

  • The free amino acid pool in Leishmania tropica promastigotes.

    abstract::The principal constituents of the free amino acid pool in Leishmania tropica promastigotes include alanine, glycine, serine, threonine, alpha-aminobutyric acid and ethanolamine. Changes in levels of the pool constituents during starvation, nutrient supplementation and treatment with a metabolic inhibitor (sodium fluor...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Simon MW,Jayasimhulu K,Mukkada AJ

    更新日期:1983-09-01 00:00:00

  • Surface-associated antigens of Brugia malayi L2 and L3 parasites during vector-stage development.

    abstract::Surface and metabolic labeling procedures were used to characterize the composition and the time of expression of Brugia malayi L2 and L3 surface-associated molecules as the larvae develop within the mosquito vector. Larvae were harvested from mosquito tissues at 5 (early L2), 8 (late L2) and 11 (L3) days post-infecti...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ibrahim MS,Richie TL,Scott AL

    更新日期:1992-05-01 00:00:00

  • Silencing of a putative inner arm dynein heavy chain results in flagellar immotility in Trypanosoma brucei.

    abstract::The Trypanosoma brucei flagellum controls motility and is crucial for cell polarity and division. Unique features of trypanosome motility suggest that flagellar beat regulation in this organism is unusual and worthy of study. The flagellar axoneme, required for motility, has a structure that is highly conserved among ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Springer AL,Bruhn DF,Kinzel KW,Rosenthal NF,Zukas R,Klingbeil MM

    更新日期:2011-01-01 00:00:00

  • A developmentally regulated cysteine protease gene family in Haemonchus contortus.

    abstract::The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

    更新日期:1990-12-01 00:00:00

  • Molecular biology of the hexokinase isoenzyme pattern that distinguishes pathogenic Entamoeba histolytica from nonpathogenic Entamoeba dispar.

    abstract::The electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish Entamoeba histolytica from Entamoeba dispar isolates. Although E. histolytica and E. dispar, previously called pathogenic and nonpathogenic Entamoeba histolytica, differ clearly in sequences of many homologous genes,...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortner S,Clark CG,Binder M,Scheiner O,Wiedermann G,Duchêne M

    更新日期:1997-05-01 00:00:00

  • The Haemonchus contortus UNC-49B subunit possesses the residues required for GABA sensitivity in homomeric and heteromeric channels.

    abstract::Hco-UNC-49 is a GABA receptor from the parasitic nematode Haemonchus contortus that has a relatively low overall sequence similarity to vertebrate GABA receptors but is very similar to the UNC-49 receptor found in the free living nematode Caenorhabditis elegans. While the nematode receptors do share >80% sequence simi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Accardi MV,Forrester SG

    更新日期:2011-07-01 00:00:00

  • The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis.

    abstract::The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

    更新日期:1988-10-01 00:00:00

  • Microsatellite polymorphism in the sexually transmitted human pathogen Trichomonas vaginalis indicates a genetically diverse parasite.

    abstract::Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

    更新日期:2011-01-01 00:00:00

  • Energy metabolism in Cotugnia digonopora and the effect of anthelmintics.

    abstract::Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pampori NA,Singh G,Srivastava VM

    更新日期:1984-04-01 00:00:00

  • PI(3)P-independent and -dependent pathways function together in a vacuolar translocation sequence to target malarial proteins to the host erythrocyte.

    abstract::Malaria parasites export 'a secretome' of hundreds of proteins, including major virulence determinants, from their endoplasmic reticulum (ER), past the parasite plasma and vacuolar membranes to the host erythrocyte. The export mechanism is high affinity (nanomolar) binding of a host (cell) targeting (HT) motif RxLxE/D...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

    更新日期:2012-10-01 00:00:00

  • Biochemical properties of a 24 kilodalton membrane glycoprotein antigen complex from Schistosoma mansoni.

    abstract::Antibodies were affinity purified from crude antiserum by elution from the 24 kDa region of preparative one-dimensional Western blots containing immobilized adult Schistosoma mansoni inner bilayer membrane proteins. They were shown to be specific for a single acidic polypeptide complex, Smgp24, following immunoblottin...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Karcz SR,Barnard BJ,Podesta RB

    更新日期:1988-11-01 00:00:00

  • Genomic organisation of nuclear tRNAGly and tRNALeu genes in Trypanosoma brucei.

    abstract::We have isolated a 0.3-kb HaeIII restriction fragment from Trypanosoma brucei which contains two tRNA genes. Secondary structure models predict that the two genes identified encode tRNA molecules which specify glycine (anticodon UCC) and leucine (anticodon CAG). The two genes are separated by 86 nucleotides, transcrib...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campbell DA,Suyama Y,Simpson L

    更新日期:1989-12-01 00:00:00

  • Antagonism of sulfadoxine and pyrimethamine antimalarial activity in vitro by p-aminobenzoic acid, p-aminobenzoylglutamic acid and folic acid.

    abstract::The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Watkins WM,Sixsmith DG,Chulay JD,Spencer HC

    更新日期:1985-01-01 00:00:00

  • Loss of matrix metalloproteinase 9 activity in Theileria annulata-attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species.

    abstract::The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prol...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adamson R,Logan M,Kinnaird J,Langsley G,Hall R

    更新日期:2000-02-25 00:00:00

  • A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes.

    abstract::During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common 'PRESAN' domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tarr SJ,Moon RW,Hardege I,Osborne AR

    更新日期:2014-08-01 00:00:00

  • 3-Methyladenine blocks Toxoplasma gondii division prior to centrosome replication.

    abstract::The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

    更新日期:2010-10-01 00:00:00

  • Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein.

    abstract::An immunodominant 72-kDa surface glycoprotein (Gp72) of Trypanosoma cruzi is involved in adhesion of the flagellum to the cell body (Cooper, R, Ribeiro de Jesus, A and Cross, G.A.M (1993) J. Cell Biol. 122, 149-156). We have characterized a gene, flagellum-adhesion glycoprotein genel (fla1), from Trypanosoma brucei th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nozaki T,Haynes PA,Cross GA

    更新日期:1996-11-25 00:00:00

  • Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins.

    abstract::The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ismaïli N,Pérez-Morga D,Walsh P,Mayeda A,Pays A,Tebabi P,Krainer AR,Pays E

    更新日期:1999-07-30 00:00:00

  • Predicted structure of a major Schistosoma mansoni eggshell protein.

    abstract::The complete sequence for a major Schistosome mansoni eggshell protein gene has been determined from a genomic DNA fragment. The use of an open reading frame encoding a glycine-rich polypeptide was confirmed by in vitro translation of schistosome mRNA in the presence of [3H]glycine and comparison with the amino acid c...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rodrigues V,Chaudhri M,Knight M,Meadows H,Chambers AE,Taylor WR,Kelly C,Simpson AJ

    更新日期:1989-01-01 00:00:00

  • A gene-family encoding small exported proteins is conserved across Plasmodium genus.

    abstract::A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

    更新日期:2003-02-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • The proteasome-ubiquitin pathway in the Schistosoma mansoni egg has development- and morphology-specific characteristics.

    abstract::Schistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mathieson W,Castro-Borges W,Wilson RA

    更新日期:2011-02-01 00:00:00