Functional characterisation of a nematode secreted GM2-activator protein.

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00

abstract：:A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

journal_title：Molecular and biochemical parasitology

authors： Hongli Y,Shuhan S,Ruiwen C,Yingjun G

更新日期：2002-01-01 00:00:00

abstract：:Two dimensional electrophoresis has been used to examine protein variation in clones of two Plasmodium falciparum isolates. Variant forms of 12 proteins were detected. Five genetically distinct parasite types were identified in one isolate, and two in the second isolate. Examination of uncloned parasites using this te...

journal_title：Molecular and biochemical parasitology

authors： Fenton B,Walker A,Walliker D

更新日期：1985-08-01 00:00:00

abstract：:With the rapid spread of drug-resistant strains of Plasmodium falciparum, the development of new antimalarials is an urgent need. As malaria parasites live in a highly pro-oxidant environment, their anti-oxidant defences have frequently been suggested as candidate drug targets. A key point in such defences is the prod...

journal_title：Molecular and biochemical parasitology

authors： Aparicio IM,Marín-Menéndez A,Bell A,Engel PC

更新日期：2010-08-01 00:00:00

abstract：:The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

journal_title：Molecular and biochemical parasitology

authors： Bishop RP

更新日期：1990-01-01 00:00:00

abstract：:Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

journal_title：Molecular and biochemical parasitology

authors： Walker R Jr,Saha L,Hill GC,Chaudhuri M

更新日期：2005-02-01 00:00:00

abstract：:The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

journal_title：Molecular and biochemical parasitology

authors： Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

更新日期：1994-10-01 00:00:00

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

journal_title：Molecular and biochemical parasitology

authors： Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

更新日期：2016-01-01 00:00:00

abstract：:The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

journal_title：Molecular and biochemical parasitology

authors： Cameron S,Fyffe SA,Goldie S,Hunter WN

更新日期：2008-04-01 00:00:00

abstract：:In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

journal_title：Molecular and biochemical parasitology

authors： Köhler P,Bachmann R

更新日期：1980-04-01 00:00:00

abstract：:Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

journal_title：Molecular and biochemical parasitology

authors： Rayner JC,Huber CS,Galinski MR,Barnwell JW

更新日期：2004-02-01 00:00:00

abstract：:Evidence is presented that Giardia lamblia and Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or...

journal_title：Molecular and biochemical parasitology

authors： Gillin FD,Reiner DS,Levy RB,Henkart PA

更新日期：1984-09-01 00:00:00

abstract：:Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A...

journal_title：Molecular and biochemical parasitology

authors： Arbabi M,Hooshyar H,Lotfinia M,Bakhshi MA

更新日期：2020-03-01 00:00:00

abstract：:The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We hav...

journal_title：Molecular and biochemical parasitology

authors： Lahti CJ,Bradley PJ,Johnson PJ

更新日期：1994-08-01 00:00:00

abstract：:Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

journal_title：Molecular and biochemical parasitology

authors： Handman E,Hocking RE,Mitchell GF,Spithill TW

更新日期：1983-02-01 00:00:00

abstract：:The karyotype and chromosomes of the human malaria parasite Plasmodium falciparum have been well characterized in recent years. Here we present karyotype maps of the three other human malaria species, P. vivax, P. malariae and P. ovale. Chromosomes of these species were found to be of significantly higher molecular we...

journal_title：Molecular and biochemical parasitology

authors： Carlton JM,Galinski MR,Barnwell JW,Dame JB

更新日期：1999-06-25 00:00:00

abstract：:Schistosoma mansoni schistosomula cultured in the presence of phospholipids showed a decreased sensitivity to the lethal complement-mediated action of anti-schistosome antibodies. Phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine had a protective action on the schistosomula transformed in vitro by pass...

journal_title：Molecular and biochemical parasitology

authors： Billecocq A

更新日期：1987-09-01 00:00:00

abstract：:Theileria annulata is a tick-transmitted protozoan that causes tropical theileriosis, an often fatal leukoproliferative disorder of cattle. To characterize and identify parasite proteins suitable as diagnostic antigens and/or vaccine candidates, a cDNA clone encoding a macroschizont stage protein was isolated and char...

journal_title：Molecular and biochemical parasitology

authors： Schnittger L,Katzer F,Biermann R,Shayan P,Boguslawski K,McKellar S,Beyer D,Shiels BR,Ahmed JS

更新日期：2002-04-09 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00

abstract：:Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

journal_title：Molecular and biochemical parasitology

authors： Chiba Y,Makiuchi T,Jeelani G,Nozaki T

更新日期：2016-06-01 00:00:00

abstract：:The number and expression of some of the large ribosomal RNA (rRNA) gene classes present in the human malaria parasite Plasmodium falciparum was determined. Southern blot analyses, using the 5.8S rRNA coding region as a marker, indicate that the P. falciparum genome contains at least 5 distinct subclasses of large rRN...

journal_title：Molecular and biochemical parasitology

authors： Shippen-Lentz D,Afroze T,Vezza AC

更新日期：1990-01-01 00:00:00

abstract：:The phylum Nematoda comprises a diverse group of roundworms that includes parasites of vertebrates, invertebrates, and plants. Human-parasitic nematodes infect more than one billion people worldwide and cause some of the most common neglected tropical diseases, particularly in low-resource countries [1]. Parasitic nem...

journal_title：Molecular and biochemical parasitology

authors： Gang SS,Hallem EA

更新日期：2016-07-01 00:00:00

abstract：:The microaerophilic parasite Trichomonas vaginalis is a causative agent of painful vaginitis or urethritis, termed trichomoniasis, and can also cause preterm delivery or stillbirth. Treatment of trichomoniasis is almost exclusively based on the nitroimidazole drugs metronidazole and tinidazole. Metronidazole resistanc...

journal_title：Molecular and biochemical parasitology

authors： Leitsch D,Drinić M,Kolarich D,Duchêne M

更新日期：2012-06-01 00:00:00

abstract：:A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

journal_title：Molecular and biochemical parasitology

authors： Liu X,Chang KP

更新日期：1994-08-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00

abstract：:Trypanosoma brucei Tim17(TbTim17), the single member of the Tim17/23/22 protein family, is an essential component of the translocase of the mitochondrial inner membrane (TIM). In spite of the conserved secondary structure, the primary sequence of TbTim17, particularly the N-terminal hydrophilic region, is significantl...

journal_title：Molecular and biochemical parasitology

authors： Weems E,Singha UK,Smith JT,Chaudhuri M

更新日期：2017-12-01 00:00:00

abstract：:Phosphatidylcholine is the most abundant phospholipid in the membranes of the human parasite Leishmania. The metabolic pathways leading to its biosynthesis are likely to play a critical role in parasite development and survival and may offer a good target for antileishmanial chemotherapy. Phosphatidylcholine synthesis...

journal_title：Molecular and biochemical parasitology

authors： Zufferey R,Mamoun CB

更新日期：2002-11-01 00:00:00

abstract：:Transfection has facilitated a functional analysis of transcriptional processes in the human malarial parasite Plasmodium falciparum, providing the first fascinating glimpses into the mechanisms regulating parasite development and pathogenicity. Here we review our rapidly evolving knowledge of what constitutes a promo...

journal_title：Molecular and biochemical parasitology

authors： Horrocks P,Dechering K,Lanzer M

更新日期：1998-09-15 00:00:00

abstract：:Intra-erythrocytic Plasmodium parasites digest host cell haemoglobin and use the liberated amino acids for protein synthesis. Although several endoproteases (aspartic, cysteine, and metallo-) have been shown to be involved in the initial stages of haemoglobin degradation, little is known about the steps immediately be...

journal_title：Molecular and biochemical parasitology

authors： Gavigan CS,Dalton JP,Bell A

更新日期：2001-09-28 00:00:00