The presence of galactofuranose and ribose units in asparagine-linked oligosaccharides of the digenetic trypanosomatid Endotrypanum schaudinni.

abstract：:A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-s...

journal_title：Molecular and biochemical parasitology

authors： Webster P,Mansour TE,Bieber D

更新日期：1989-10-01 00:00:00

abstract：:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P....

journal_title：Molecular and biochemical parasitology

authors： Kraemer SM,Gupta L,Smith JD

更新日期：2003-06-01 00:00:00

abstract：:The promoter region of the ribosomal RNA (rRNA) genes of Leishmania amazonensis was characterised and the transcription start point, defined by primer extension, was shown to be a T residue, 1048 nucleotides upstream of the beginning of the 18S sequence. A repetitive element of 60 bp was identified in the intergenic s...

journal_title：Molecular and biochemical parasitology

authors： Uliana SR,Fischer W,Stempliuk VA,Floeter-Winter LM

更新日期：1996-02-01 00:00:00

abstract：:The principal constituents of the free amino acid pool in Leishmania tropica promastigotes include alanine, glycine, serine, threonine, alpha-aminobutyric acid and ethanolamine. Changes in levels of the pool constituents during starvation, nutrient supplementation and treatment with a metabolic inhibitor (sodium fluor...

journal_title：Molecular and biochemical parasitology

authors： Simon MW,Jayasimhulu K,Mukkada AJ

更新日期：1983-09-01 00:00:00

abstract：:Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

journal_title：Molecular and biochemical parasitology

authors： Keegan F,Blum JJ

更新日期：1990-03-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC 3.6.1.5) which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

journal_title：Molecular and biochemical parasitology

authors： Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

更新日期：2008-12-01 00:00:00

abstract：:Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96,000, 80,000 and 50,000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of po...

journal_title：Molecular and biochemical parasitology

authors： Symons FM,Murray PJ,Ji H,Simpson RJ,Osborn AH,Cappai R,Handman E

更新日期：1994-09-01 00:00:00

abstract：:Schistosomes have a complex life cycle (vertebrate and molluscan hosts as well as larvae living freely in water) in which they are exposed to different environments and temperatures (20 degrees C - 37 degrees C). Since heat shock genes are activated in response to stress and during development [1], it is of interest t...

journal_title：Molecular and biochemical parasitology

authors： Neumann S,Ziv E,Lantner F,Schechter I

更新日期：1992-12-01 00:00:00

abstract：:The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of pu...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Galatis D,Cowman AF

更新日期：1993-04-01 00:00:00

abstract：:In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

journal_title：Molecular and biochemical parasitology

authors： Navarro M,Cross GA

更新日期：1998-07-01 00:00:00

abstract：:Neospora caninum is an obligate intracellular Apicomplexa, a phylum where one of the current methods for functional studies relies on molecular genetic tools. For Toxoplasma gondii, the first method described, in 1993, was based on resistance against chloramphenicol. As in T. gondii, we developed a vector constituted ...

journal_title：Molecular and biochemical parasitology

authors： Pereira LM,Yatsuda AP

更新日期：2014-09-01 00:00:00

abstract：:Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

journal_title：Molecular and biochemical parasitology

authors： Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

更新日期：1995-07-01 00:00:00

abstract：:Sj23, the 23-kDa target antigen in Schistosoma japonicum adult worms of the hybridoma monoclonal antibody (mAb) I-134, has been identified and cloned from cDNA libraries, mAb I-134 has been successfully used in immunodiagnostic assays to detect S. japonicum infection in Philippine patients. Sequence analysis has shown...

journal_title：Molecular and biochemical parasitology

authors： Davern KM,Wright MD,Herrmann VR,Mitchell GF

更新日期：1991-09-01 00:00:00

abstract：:The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

journal_title：Molecular and biochemical parasitology

authors： Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

更新日期：1988-02-01 00:00:00

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00

abstract：:Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

journal_title：Molecular and biochemical parasitology

authors： Kwakye-Berko F,Meshnick S

更新日期：1990-03-01 00:00:00

abstract：:The phylum Nematoda comprises a diverse group of roundworms that includes parasites of vertebrates, invertebrates, and plants. Human-parasitic nematodes infect more than one billion people worldwide and cause some of the most common neglected tropical diseases, particularly in low-resource countries [1]. Parasitic nem...

journal_title：Molecular and biochemical parasitology

authors： Gang SS,Hallem EA

更新日期：2016-07-01 00:00:00

abstract：:Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

journal_title：Molecular and biochemical parasitology

authors： Singer SM,Yee J,Nash TE

更新日期：1998-04-01 00:00:00

abstract：:Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

journal_title：Molecular and biochemical parasitology

authors： Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

更新日期：1990-11-01 00:00:00

abstract：:The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

journal_title：Molecular and biochemical parasitology

authors： Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

更新日期：1988-10-01 00:00:00

abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

journal_title：Molecular and biochemical parasitology

authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

更新日期：2010-10-01 00:00:00

abstract：:The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

journal_title：Molecular and biochemical parasitology

authors： Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

更新日期：1992-07-01 00:00:00

abstract：:Post-transcriptional regulation is a key feature controlling gene expression in the protozoan parasite Leishmania. The nine-nucleotide paraflagellar rod regulatory element (PRE) in the 3'UTR of Leishmania mexicana PFR2 is both necessary and sufficient for the observed 10-fold higher level of PFR2 mRNA in promastigotes...

journal_title：Molecular and biochemical parasitology

authors： Holzer TR,Mishra KK,LeBowitz JH,Forney JD

更新日期：2008-01-01 00:00:00

abstract：:Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

journal_title：Molecular and biochemical parasitology

authors： Cacciò SM,Ryan U

更新日期：2008-08-01 00:00:00

abstract：:Two tandemly linked genes are present in the Leishmania infantum genome that code for the acidic ribosomal PO protein. The genes are identical in the coding region, although a striking lack of nucleotide sequence conservation is observed when the boundaries of the coding regions between both genes are compared. The 3'...

journal_title：Molecular and biochemical parasitology

authors： Soto M,Requena JM,Alonso C

更新日期：1993-10-01 00:00:00

abstract：:Nucleotide-sugar transporters (NSTs) transport activated sugars (e.g. UDP-GlcNAc) from the cytosol to the lumen of the endoplasmic reticulum or Golgi apparatus where they are used to make glycoproteins and glycolipids. UDP-Glc is an important component of the N-glycan-dependent quality control (QC) system for protein ...

journal_title：Molecular and biochemical parasitology

authors： Banerjee S,Cui J,Robbins PW,Samuelson J

更新日期：2008-05-01 00:00:00

abstract：:The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

journal_title：Molecular and biochemical parasitology

authors： Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

更新日期：2001-08-01 00:00:00

abstract：:Leishmania parasites cycle between the alimentary tract of a sandfly vector as free-living promastigotes and the acidified phagolysosomes of the vertebrate host macrophage as aflagellated amastigotes. The differentiation process can be mimicked in host-free culture by switching promastigotes (e.g. 25 degrees C, neutra...

journal_title：Molecular and biochemical parasitology

authors： Rochette A,Raymond F,Corbeil J,Ouellette M,Papadopoulou B

更新日期：2009-05-01 00:00:00

abstract：:The merozoite surface protein-1 of the human malaria parasite Plasmodium falciparum undergoes an extracellular proteolytic cleavage (secondary processing) intrinsic to successful erythrocyte invasion. In the T9/96 clone of P. falciparum the protease responsible has been characterised as a membrane-associated, calcium-...

journal_title：Molecular and biochemical parasitology

authors： Blackman MJ,Chappel JA,Shai S,Holder AA

更新日期：1993-11-01 00:00:00