Isolation and characterization of a lectin from Sarcocystis gigantea.

abstract：:A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

journal_title：Molecular and biochemical parasitology

authors： Liu X,Chang KP

更新日期：1994-08-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00

abstract：:The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

journal_title：Molecular and biochemical parasitology

authors： DaRocha WD,Otsu K,Teixeira SM,Donelson JE

更新日期：2004-02-01 00:00:00

abstract：:Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

journal_title：Molecular and biochemical parasitology

authors： Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

更新日期：2001-11-01 00:00:00

abstract：:Cathepsin L-like activity was demonstrated in the excretory/secretory (E/S) products of Fasciola hepatica newly excysted juveniles (NEJ), 3-week-old, 5-week-old and mature flukes using the fluorogenic substituted 7-amino-4-methylcoumarin substrates Z-phe-arg-AMC, Z-arg-arg-AMC and Z-arg-AMC. Gelatin-substrate polyacry...

journal_title：Molecular and biochemical parasitology

authors： Carmona C,Dowd AJ,Smith AM,Dalton JP

更新日期：1993-11-01 00:00:00

abstract：:As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed o...

journal_title：Molecular and biochemical parasitology

authors： Robertson CD

更新日期：1999-09-20 00:00:00

abstract：:Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

journal_title：Molecular and biochemical parasitology

authors： Engelmann S,Sinnis P,Matuschewski K

更新日期：2006-03-01 00:00:00

abstract：:Plasmodium falciparum merozoites invade erythrocytes using a range of alternative ligands that includes erythrocyte binding antigenic proteins (EBAs) and reticulocyte binding protein homologues (Rh). Variation in the expression of some of these genes among culture-adapted parasite lines correlates with the use of diff...

journal_title：Molecular and biochemical parasitology

authors： Nery S,Deans AM,Mosobo M,Marsh K,Rowe JA,Conway DJ

更新日期：2006-10-01 00:00:00

abstract：:A screen for Leishmania mexicana genes encoding promastigote-specific flagellar proteins resulted in isolation of genes encoding the major components of the paraflagellar rod. One of these, PFR-2, was characterized extensively. PFR-2 genes are present in the genome as a tandem array of three genes designated PFR-2A, P...

journal_title：Molecular and biochemical parasitology

authors： Moore LL,Santrich C,LeBowitz JH

更新日期：1996-10-01 00:00:00

abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

journal_title：Molecular and biochemical parasitology

authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

更新日期：2000-12-01 00:00:00

abstract：:The exoglycosidase, beta-N-acetyl-D-hexosaminidase was purified 600-fold from the muscle-stage larvae (L1) of Trichinella spiralis. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 5...

journal_title：Molecular and biochemical parasitology

authors： Rhoads ML

更新日期：1988-10-01 00:00:00

abstract：:Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

journal_title：Molecular and biochemical parasitology

authors： Das A

更新日期：1993-08-01 00:00:00

abstract：:Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

journal_title：Molecular and biochemical parasitology

authors： Sugisaki H,Ray DS

更新日期：1987-04-01 00:00:00

abstract：:Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

journal_title：Molecular and biochemical parasitology

authors： Uttaro AD,Opperdoes FR

更新日期：1997-10-01 00:00:00

abstract：:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

journal_title：Molecular and biochemical parasitology

authors： Thompson SN,Platzer EG,Lee RW

更新日期：1987-01-02 00:00:00

abstract：:Cystic hydatid disease (CHD) is caused by infection with Echinococcus granulosus metacestodes and affects humans and livestock. Proteins secreted or excreted by protoscoleces, pre-adult worms found in the metacestode, are thought to play fundamental roles in the host-parasite relationship. In this work, we performed a...

journal_title：Molecular and biochemical parasitology

authors： Virginio VG,Monteiro KM,Drumond F,de Carvalho MO,Vargas DM,Zaha A,Ferreira HB

更新日期：2012-05-01 00:00:00

abstract：:A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In the parasitized erythrocyte, PfCG2 expression rises sharply in the trophozoite stage and is detected in electron-dense patches along the parasitophorous vacuolar membrane (PVM), in the cyto...

journal_title：Molecular and biochemical parasitology

authors： Cooper RA,Papakrivos J,Lane KD,Fujioka H,Lingelbach K,Wellems TE

更新日期：2005-12-01 00:00:00

abstract：:The FAR proteins of nematodes are small ( approximately 20 kDa), helix-rich, fatty acid and retinol-binding (FAR) proteins that appear to be confined to nematodes. We have carried out a comparative sequence and biochemical analysis of selected FAR proteins often species of filarial parasites (from the genera Onchocerc...

journal_title：Molecular and biochemical parasitology

authors： Garofalo A,Kläger SL,Rowlinson MC,Nirmalan N,Klion A,Allen JE,Kennedy MW,Bradley JE

更新日期：2002-07-01 00:00:00

abstract：:Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectro...

journal_title：Molecular and biochemical parasitology

authors： Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KD

更新日期：2009-12-01 00:00:00

abstract：:The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

journal_title：Molecular and biochemical parasitology

authors： Barral V,This P,Imbert-Establet D,Combes C,Delseny M

更新日期：1993-06-01 00:00:00

abstract：:Phosphatidylcholine is the most abundant phospholipid in the membranes of the human parasite Leishmania. The metabolic pathways leading to its biosynthesis are likely to play a critical role in parasite development and survival and may offer a good target for antileishmanial chemotherapy. Phosphatidylcholine synthesis...

journal_title：Molecular and biochemical parasitology

authors： Zufferey R,Mamoun CB

更新日期：2002-11-01 00:00:00

abstract：:Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/P...

journal_title：Molecular and biochemical parasitology

authors： Garcia-Silva MR,Frugier M,Tosar JP,Correa-Dominguez A,Ronalte-Alves L,Parodi-Talice A,Rovira C,Robello C,Goldenberg S,Cayota A

更新日期：2010-06-01 00:00:00

abstract：:The cytotoxicity of ricin and diphtheria toxin was studied in culture-adapted bloodstream forms of Trypanosoma brucei. Although ricin is endocytosed at a rate comparable to that of other internalized macromolecules, it is nontoxic to bloodstream-form trypanosomes. The resistance lies partly in low susceptibility of th...

journal_title：Molecular and biochemical parasitology

authors： Scory S,Steverding D

更新日期：1997-12-01 00:00:00

abstract：:The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

journal_title：Molecular and biochemical parasitology

authors： Ortiz G,Segovia M

更新日期：1996-11-25 00:00:00

abstract：:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

journal_title：Molecular and biochemical parasitology

authors： Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

更新日期：1990-12-01 00:00:00

abstract：:The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We hav...

journal_title：Molecular and biochemical parasitology

authors： Lahti CJ,Bradley PJ,Johnson PJ

更新日期：1994-08-01 00:00:00

abstract：:The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

journal_title：Molecular and biochemical parasitology

authors： Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

更新日期：1992-07-01 00:00:00

abstract：:A complex of non-covalently bound polypeptides is located on the surface of the merozoite form of the human malaria parasite Plasmodium falciparum. Four of these polypeptides are derived by proteolytic processing of the merozoite surface protein 1 (MSP-1) precursor. Two components, a 22 and a 36 kDa polypeptide are no...

journal_title：Molecular and biochemical parasitology

authors： Trucco C,Fernandez-Reyes D,Howell S,Stafford WH,Scott-Finnigan TJ,Grainger M,Ogun SA,Taylor WR,Holder AA

更新日期：2001-01-15 00:00:00

abstract：:Natural antisense transcripts (NATs) are non-protein coding RNAs that could play an important role in regulating the expression of their counterpart protein encoding sense transcript. Although NATs are widespread in most eukaryotic genomes, very little is known about their functions. This study focuses on gaining a be...

journal_title：Molecular and biochemical parasitology

authors： Fahim A,Afrin F,Wen G,Ananvoranich S

更新日期：2020-11-01 00:00:00

abstract：:Two dimensional electrophoresis has been used to examine protein variation in clones of two Plasmodium falciparum isolates. Variant forms of 12 proteins were detected. Five genetically distinct parasite types were identified in one isolate, and two in the second isolate. Examination of uncloned parasites using this te...

journal_title：Molecular and biochemical parasitology

authors： Fenton B,Walker A,Walliker D

更新日期：1985-08-01 00:00:00