Abstract:
:Antibody-mediated elimination of foreign antigens contributes to immune protection from viral infection. We have generated phycoerythrin-conjugated HIV-1 Gag/p24 and influenza virus nucleoprotein tetramers for flow cytometric analysis of blood cell populations involved in antibody-mediated immunity. We show that in the presence of antigen-specific antibodies fluorescent antigen tetramers bound to different blood cell populations including granulocytes, monocytes and lymphocytes. Binding to B-lymphocytes was particularly efficient. The interaction was primarily mediated by complement. Fcgamma receptor-mediated antigen binding by B-cells was significantly less effective. The study shows that fluorescent antigen tetramers are useful to quantify cell populations involved in complement- and Fcgamma-mediated immune responses in viral infections.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Reiche S,Bussmann BM,Dwai Y,Jassoy Cdoi
10.1016/j.imbio.2009.02.004subject
Has Abstractpub_date
2010-03-01 00:00:00pages
223-9issue
3eissn
0171-2985issn
1878-3279pii
S0171-2985(09)00040-0journal_volume
215pub_type
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