Abstract:
:Human T-lymphocyte clones showing proliferative and cytotoxic responses specific for HLA antigens were isolated. To obtain enriched numbers of functionally active colonies, the cells were first sensitized in vitro in a tertiary mixed lymphocyte culture (MLC), using 10 stimulating cells for each responding cell. To assess quickly the genetic specificity of the clones, the cells used in the priming MLC and later as screening cells were prepared from an HLA-typed family. Using this approach, 75% of the colonies seeded at 1 cell/well showed functional activity specific for HLA.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Schendel DJ,Wank Rdoi
10.1016/s0171-2985(81)80062-9subject
Has Abstractpub_date
1981-01-01 00:00:00pages
367-71issue
3-4eissn
0171-2985issn
1878-3279pii
S0171-2985(81)80062-9journal_volume
160pub_type
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