Abstract:
OBJECTIVE:Vascular endothelial growth factor is a potent stimulator of angiogenesis; activation of hypoxia inducible factor-1alpha and p44/42 mitogen-activated protein kinase pathways has been associated with vascular endothelial growth factor production. The present study examined if thrombin modulates vascular endothelial growth factor levels in gliomas via hypoxia inducible factor-1alpha and p44/42 mitogen-activated protein kinase pathways. METHODS:This study was divided into two parts: (1) adult male Fischer 344 rats had an intracaudatal implantation of C6 glioma cells. Rats were killed 6, 9 and 12 days later for Western blotting and immunohistochemistry. Hypoxia inducible factor-1alpha messenger ribonucleic acid was also determined; (2) cultured C6 cells were treated with vehicle, thrombin, thrombin plus YC-1, an inhibitor of hypoxia inducible factor-1alpha, or thrombin plus PD098059, an inhibitor of p44/42 mitogen-activated protein kinases. Cell culture medium was collected for vascular endothelial growth factor measurement using an enzyme-linked immunosorbent assay kit. RESULTS:In vivo, immunoreactivities for thrombin, vascular endothelial growth factor, hypoxia inducible factor-1alpha and activated p44/42 mitogen-activated protein kinases were detected in C6 gliomas. Thrombin immunoreactivity was co-localized with that of vascular endothelial growth factor, hypoxia inducible factor-1alpha or activated p44/42 mitogen-activated protein kinases. A time course showed that activated p44/42 mitogen-activated protein kinases and vascular endothelial growth factor protein levels increased in gliomas with time. Hypoxia inducible factor-1alpha protein levels were higher in C6 gliomas than those in the contralateral brain tissue. In vitro, thrombin exposure increased the levels of vascular endothelial growth factor, hypoxia inducible factor-1alpha and activated p44/42 mitogen-activated protein kinases in C6 cells. Thrombin-induced vascular endothelial growth factor up-regulation was blocked by YC-1 and PD098059. DISCUSSION:Thrombin activates hypoxia inducible factor-1alpha and p44/42 mitogen-activated protein kinase pathways, and up-regulates vascular endothelial growth factor in gliomas. Inhibiting hypoxia inducible factor-1alpha and p44/42 mitogen-activated protein kinase pathways reduces the thrombin-induced up-regulation of vascular endothelial growth factor. These results suggest thrombin can increase vascular endothelial growth factor levels via activating hypoxia inducible factor-1alpha and p44/42 mitogen-activated protein kinase pathways in gliomas.
journal_name
Neurol Resjournal_title
Neurological researchauthors
Xu Y,Gu Y,Keep RF,Heth J,Muraszko KM,Xi G,Hua Ydoi
10.1179/174313209X385699subject
Has Abstractpub_date
2009-09-01 00:00:00pages
759-65issue
7eissn
0161-6412issn
1743-1328pii
ner1801journal_volume
31pub_type
杂志文章abstract:OBJECTIVES:Bone marrow stromal cells (BMSCs) have been reported to improve movement deficit in adult rats with spinal cord injury (SCI). The purpose of this study is to determine the distribution of BMSCs in the spinal cord lesion of the contusion model of SCI. METHODS:Laminectomy was carried out at L1 vertebra level ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164107X165642
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:To investigate the perfusion parameters of computed tomography (CT) in hypoxicischemic encephalopathy (HIE) after off-pump coronary artery bypass grafting (OPCABG), and its potential in predicting the occurrence and prognosis of hypoxic-ischemic encephalopathy. METHODS:We selected 830 patients. The patients ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2017.1315862
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Traumatic brain injuries (TBIs) cause a substantial burden to the patient, relatives, and the society as a whole. Much experience and knowledge during the last two decades have improved the neurosurgical treatment as well as the outcome. However, there is still much debate on what actually happens when exter...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/1743132813Y.0000000227
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVES:To review the use of immunoassays in the diagnosis and research of disorders affecting the nervous system. METHODS:Systematic review of the English literature. RESULTS:Immunoassays have demonstrated utility for: (1) the detection of antigen (molecules, genes, gene products, peptides, hormones and drug meta...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/016164105X49535
更新日期:2005-10-01 00:00:00
abstract::In addition to its possible role as a replacement source in CNS degenerative diseases, neural transplantation may be used to augment the normal production of neuroactive substances. Our laboratory at the University of Illinois at Chicago has shown, in both acute and chronic pain models, that transplantation of adrenal...
journal_title:Neurological research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1080/01616412.1997.11740775
更新日期:1997-02-01 00:00:00
abstract::We postulate that severe atherosclerotic occlusion of the circle of Willis and leptomeningeal arteries is an important factor in the pathogenesis of some sporadic Alzheimer's disease (AD) cases. These arterial stenoses are complicated by an overwhelming amyloid accumulation in the walls of leptomeningeal and cortical ...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/016164104225017668
更新日期:2004-07-01 00:00:00
abstract::We examined antioxidant activity of cabergoline, a dopamine agonist, during the aerobic oxidation of phosphatidylcholine liposomes at 37 degrees C. Cabergoline retarded the oxidation initiated with a lipid-soluble initiator significantly better than that with a water-soluble initiator, suggesting that cabergoline loca...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164104225016029
更新日期:2004-06-01 00:00:00
abstract::The spontaneous disappearance and reappearance of a ruptured cerebral aneurysm is generally assumed to be a rare phenomenon although the actual incidence is unknown. Among 39 consecutive cases of acute subarachnoid hemorrhage (SAH), 33 were studied by three-dimensional computed tomographic angiography (CTA) within 6 h...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2000.11740723
更新日期:2000-09-01 00:00:00
abstract::With recent advancement in medical imaging, techniques, and endovascular tools more patients are diagnosed with unruptured intracranial aneurysms. The main aim of offering aneurysm treatment is to ameliorate the risk of future aneurysm bleeding, while not posing additional risks on the patient from the treatment itsel...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/1743132814Y.0000000328
更新日期:2014-04-01 00:00:00
abstract::A computer software program (Seapit) was developed for precise determination of intracranial targets identified by stereotactic computed tomography (CT). This program was added to the software of a GE 8800 CT scanner to perform the following operations: millimetre precise calculation and display of the rectilinear coo...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1987.11739781
更新日期:1987-06-01 00:00:00
abstract::Before extending the application of motor cortex stimulation it is important to investigate the intimate mechanisms by which it alleviates intractable pain and to consider possible side effects. Self-mutilation in animals following extensive neurectomy or posterior rhizotomy of a limb is thought to reveal severe dyses...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164103101201274
更新日期:2003-03-01 00:00:00
abstract::Based on the successful use of fetal striatal brain grafting in the restoration of striatal function in rat and nonhuman primate models of Huntington's disease, as well as on the evidence for the clinical potential of fetal brain grafting in the treatment of Parkinson's disease, homotopic fetal striatal homotransplant...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1995.11740334
更新日期:1995-08-01 00:00:00
abstract::Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by the degeneration of the dopamine producing neurons projecting from the substantia nigra into the corpus striatum. Current medical therapy is limited and cannot stop or reverse the degeneration. Over the past 30 years, attempts were m...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/016164104225017604
更新日期:2004-06-01 00:00:00
abstract::1-Aminocyclopropanecarboxylic acid is a high affinity ligand with partial agonist properties at strychnine-insensitive glycine sites associated with the N-methyl-D-aspartate subtype of glutamate receptors. Since occupation of these sites appears required for operation of N-methyl-D-aspartate, receptor coupled cation c...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1995.11740324
更新日期:1995-08-01 00:00:00
abstract::Corticotrophin releasing factor has been shown in several models of tissue injury to be an effective treatment for edema. In a previous study we demonstrated this ability in two models of traumatic brain injury (TBI). The aim of this study was to assess whether human corticotrophin releasing factor (hCRF) could additi...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2000.11740737
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND/AIMS:Both of neuromyelitis optica spectrum disease (NMOSDs) and idiopathic transverse myelitis (ITM) could present as acute transverse myelitis. However, long-term immunological treatment and prognosis are different for high recurrence of NMOSDs. In this study, we summarized clinical differences between acut...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2020.1773628
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Patent foramen ovale (PFO) is considered as an important risk factor for cerebrovascular diseases. Nevertheless, the relationship between the distribution of high-intensity transient signals (HITS), resulting from injection of air mixed with saline and detected by transcranial Doppler (TCD), and clinical cer...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164105X18458
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVES:To study whether Clopidogrel-Aspirin combined treatment for high risk transient ischaemic attack (TIA) or minor stroke results in increased number of lesions associated with anti-thrombotic cerebral haemorrhage or cerebral micro-bleeds (CMB) than aspirin alone treatment. METHODS:The patients recruited in CH...
journal_title:Neurological research
pub_type: 杂志文章,随机对照试验
doi:10.1179/1743132815Y.0000000087
更新日期:2015-11-01 00:00:00
abstract::We examined the effect of local anaesthesia of the skin on somatosensory evoked potentials (SEPs) and psychophysical magnitude estimates for a constant intensity air-puff stimulation (7 kg.cm-2) in 23 normal subjects. Before and after intradermal injection (0.1 ml of 1% xylocain) to the tip of the right index finger, ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1995.11740283
更新日期:1995-02-01 00:00:00
abstract::The present study was designed to investigate the beneficial effects of cornel iridoid glycoside (CIG), a main component extract from Cornus officinalis, on neurotrophin expression in mouse experimental autoimmune encephalomyelitis (EAE), a classical model of multiple sclerosis (MS). After EAE initiation, CIG was intr...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2016.1200766
更新日期:2016-09-01 00:00:00
abstract::Mild vascular cognitive impairment (mVCI) is a broader term that is intended to detect cognitive loss before the development of dementia. The identification of preventable risk factors as well as therapeutic strategies of intervention is still unclear. It has been suggested that carotid endarterectomy (CEA) improves c...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164104225016245
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVES:We reviewed our most recent work on the protective effect of adenosine A(2A)antagonism in cerebral ischemia. METHODS:Focal ischemia was produced in rats by introducing a nylon monofilament pre-coated with silicone through the external carotid artery to occlude the right MCA at its origin. RESULTS:A(2A) ant...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/016164105X21913
更新日期:2005-03-01 00:00:00
abstract::The present study was performed to compare brain infarct size assessment by routine histology (haematoxylin and eosin) and by 2,3,5-triphenyltetrazolium chloride staining techniques. New Zealand white rabbits were subjected to autologous clot embolization to the anterior circulation of the brain. After a study period ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1994.11740210
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVES:In the present study, we investigated the effects of treadmill exercise on microglial activation and the subsequent release of tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-6 and IL-1-beta in the hippocampus in a rat model of type 2 diabetes. METHODS:At 30 weeks of age, diabetic (Zucker diabeti...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/1743132815Y.0000000015
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVES:This study sought to compare the atherosclerotic plaque burden between middle cerebral artery (MCA) and extracranial carotid artery (ECA) in ischemic stroke patients using high-resolution, black-blood (HR BB) MRI and to investigate the relationship between plaque burden found in both arteries and stroke seve...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2017.1281196
更新日期:2017-04-01 00:00:00
abstract::The survival of a group of patients with malignant gliomas of the brain submitted to radical surgery and radiotherapy was studied. The average survival was nearly 4 times longer with X-ray treatment than with cobalt 60 irradiation when a comparison was made between 24 patients with glioblasto-astroblastoma (grade 3-4)...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1987.11739796
更新日期:1987-09-01 00:00:00
abstract:OBJECTIVES:Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder, characterized by a selective progressive degeneration of the motor system. Electromyography is essential for the diagnosis of ALS. The measurement of motor conduction of peripheral nerves is of major importance to recognize other poss...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164107X159261
更新日期:2007-07-01 00:00:00
abstract:OBJECTIVES:The utility of apparent diffusion coefficient (ADC) values was evaluated for the differential diagnosis of ganglioglioma. METHODS:Ten consecutive patients, five men and five women, aged from 14 to 67 years, with histologically proven supratentorial gangliogliomas, underwent diffusion-weighted magnetic reson...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/174313209X382539
更新日期:2009-12-01 00:00:00
abstract::Analysis of the blood flow velocities in the middle cerebral artery by transcranial Doppler ultrasonography was performed in 158 healthy volunteers (aged 14-70 years; 82 men and 76 women). In a subgroup of 38 men and 21 women the end-tidal [CO2] was also measured. The influence of biological factors such as age, sex, ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1990.11739941
更新日期:1990-09-01 00:00:00
abstract::Aspirin and ticlopidine are two commonly used drugs in the prevention of cerebral embolic ischemic events. No direct comparisons in a cross-over design of the effects of ticlopidine and aspirin on asymptomatic circulating cerebral microemboli are available. We investigated 53 patients with cerebrovascular disease. Twe...
journal_title:Neurological research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/01616412.1996.11740450
更新日期:1996-10-01 00:00:00