Abstract:
:DNA mismatch repair (MMR) is critical for the maintenance of genomic stability. MMR is initiated by recognition of DNA mismatches by the protein, MutS, which subsequently recruits downstream repair factors. To better understand the mechanism by which MutS identifies and specifically binds mismatched basepairs embedded in random DNA sequences, we monitored the interaction between MutS and DNA substrates using atomic force microscopy (AFM). An alpha-shaped DNA loop formed by the interaction between MutS and DNA, which was independent of whether or not a mismatch was present in the DNA substrate. These data indicate that MutS associates with DNA non-specifically and forms an alpha-loop interaction with the DNA substrate. In this conformation, MutS is able to scan two arms of DNA simultaneously for each MutS dimer formed.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Jia Y,Bi L,Li F,Chen Y,Zhang C,Zhang Xdoi
10.1016/j.bbrc.2008.05.093subject
Has Abstractpub_date
2008-08-08 00:00:00pages
618-22issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(08)00989-3journal_volume
372pub_type
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