Abstract:
:Chimerism analysis has become an important tool to manage patients in the peri-transplant period of allogenic stem cell transplantation. During this period, cells of donor and host origin can coexist and increasing proportion of cells of host origin is considered as a recurrence of the underlying disease. We currently performed chimerism analysis on separate peripheral blood cell subsets, lymphocytes and granulocytes. To improve our isolation method, a new automated device from Stem Cell Technology Roboseptrade mark was tested and compared to our manual separation technique. The results obtained on T cell purification showed an improvement of the purity (98.42% with Robosep vs. 92.42% with the manual technique Rosettesep) and of the recovery (63.43% with Robosep and 38% with Rosettesep). The results were significantly improved on patient samples with less than 10% CD3 positive cells (purity: 90% vs. 44.44%; recovery: 73.79% vs. 43.98%). Granulocytes separation was based on CD15 expression. The results showed an improvement of the purity with Robosep (96.90% vs. 86.20% with the manual technique Polymorphprep) but the recovery was impaired (35.2% vs. 52.30%). Using a myeloid (CD66/CD33) cocktail, recovery was improved with the Robosep device (64.04% with the myeloid cocktail vs. 22.4% with the CD15 cocktail). Our data demonstrated that Robosep allowed a performant cell purification in the early period post-transplantation even for populations representing less than 10% of the peripheral blood cells.
journal_name
Biomed Mater Engjournal_title
Bio-medical materials and engineeringauthors
Decot V,Latger-Cannard V,Lecompte T,Clément L,Salmon A,Bordigoni P,Stoltz JF,Bensoussan Dsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
S19-26issue
1 Suppleissn
0959-2989issn
1878-3619journal_volume
18pub_type
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130907
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-2011-0671
更新日期:2011-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141003
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-171673
更新日期:2017-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141123
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130833
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130741
更新日期:2013-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Based upon previous research on the relation between hip prosthesis designs and strain distributions in a proximal femur model, a prototype of a "physiological" hip prosthesis was designed and manufactured. Strain gauge measurements on a dry femur before and after implantation of this prosthesis were made in different...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::Recognition of lung cancer cells is very important to the clinical diagnosis of lung cancer. In this paper we present a novel method to extract the structure characteristics of lung cancer cells and automatically recognize their types. Firstly soft mathematical morphology methods are used to enhance the grayscale imag...
journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-2009-0603
更新日期:2009-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-141093
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章,评审
doi:10.3233/BME-161568
更新日期:2016-05-12 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130886
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130863
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-161592
更新日期:2016-09-28 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-151491
更新日期:2015-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-130927
更新日期:2014-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-181034
更新日期:2019-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:10.3233/BME-201079
更新日期:2020-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00