Abstract:
:Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or the nucleus in 3T3-L1 adipocytes are investigated using a series of truncated mutants of Prp19p that were fused to EGFP and transiently introduced into differentiated 3T3-L1 adipocytes. Immunofluorescence microscopy revealed that a domain of amino acids 167-250 is necessary for the recruitment of Prp19p to lipid droplets and that a domain of amino acids 1-166 is necessary for the recruitment of Prp19p to a nucleus.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Cho SY,Park PJ,Lee JH,Kim JJ,Lee TRdoi
10.1016/j.bbrc.2007.10.091subject
Has Abstractpub_date
2007-12-28 00:00:00pages
844-9issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)02245-0journal_volume
364pub_type
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