Abstract:
:CART (cocaine- and amphetamine-regulated transcript) peptides are neuropeptides abundant in the central nervous system and periphery found to be involved in the regulation of food intake behavior and other physiological processes. Recently, we reported specific binding of (125)I-CART(61-102) to the rat adrenal pheochromocytoma cell line PC12, both intact cells and cell membranes. In this study, several fragments of CART(61-102) corresponding to its structural loops were synthesized and tested for their potency in binding experiments using PC12 intact cells and cell membranes and in feeding test with fasted mice. From all shorter peptides tested, only CART(74-86) and CART(62-86) containing disulfide bridges kept partial binding potency of the original molecule with K(i) in 10(-5) and 10(-4)M range. However, these fragments were not able to inhibit food intake after their central administration up to a dose of 4 nmol/mouse. The results showed that a compact structure containing three disulfide bridges is necessary for preservation of full biological activity of CART peptides.
journal_name
Peptidesjournal_title
Peptidesauthors
Maixnerová J,Hlavácek J,Blokesová D,Kowalczyk W,Elbert T,Sanda M,Blechová M,Zelezná B,Slaninová J,Maletínská Ldoi
10.1016/j.peptides.2007.07.022subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1945-53issue
10eissn
0196-9781issn
1873-5169pii
S0196-9781(07)00269-0journal_volume
28pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::The present status of our understanding of the feedback regulation of pancreatic secretion by peptide YY (PYY) released from the distal intestine is reviewed. Exocrine pancreatic secretion is primarily controlled by the cephalic (the vagus nerve), gastric (acid and pepsin secretion, and nutrients delivered into the du...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(01)00612-x
更新日期:2002-02-01 00:00:00
abstract::We have cloned melanocortin receptors (MCRs) from several species of fish. The MC4R and MC5R subtypes arose early in vertebrate evolution and their primary structure is remarkably conserved. Expression and pharmacological characterization of the MCRs in fish has revealed that they bind and respond to melanocortin pept...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2004.11.034
更新日期:2005-10-01 00:00:00
abstract::Using the indirect immunofluorescence method, enkephalin-like immunoreactivity was visualized on human fetus spinal cord sections (gestational age from 17 to 25 weeks). Immunolabeled varicose fibers and terminal-like structures were seen through the whole length fetal spinal cord principally in the dorsal gray, in the...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90018-6
更新日期:1988-01-01 00:00:00
abstract::It is well known that neuropeptide Y (NPY) and gamma-aminobutyric acid (GABA) exert antiepileptic effects in animal models. It has recently been shown that ghrelin neurons increase the activities of GABA and NPY in the brain. Therefore it can be said that ghrelin is an antiepileptic agent. In this study we aimed to in...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.04.003
更新日期:2007-06-01 00:00:00
abstract::Antibodies to the extreme C-terminal pentapeptide of procholecystokinin, YEYPS (in the single letter notation), have been used in radioimmunoassay and immunohistochemistry to characterize the material in rat intestine. There is a single major immunoreactive peptide in intestinal extracts that has the properties of the...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90170-7
更新日期:1987-01-01 00:00:00
abstract::This study was designed to investigate the effects of synthetic mouse pancreatic polypeptide (mPP) on feeding and anxiety in mice. The intracerebroventricular (i.c.v.) injection of mPP (0.003-3 nmol) dose-dependently increased food intake. A significant increase was observed 20 min after i.c.v. injection and continued...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00155-2
更新日期:1999-12-01 00:00:00
abstract::Morphologic and immunohistochemical studies were conducted to ascertain whether pumiliotoxin-B (PTX-B), an indolizine alkaloid from the skin of the Neotropical dendrobatid frog, Dendrobates pumilio, affects the anatomic and immunohistochemical features of the electrically stimulated mouse vas deferens preparations. PT...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00066-2
更新日期:1999-01-01 00:00:00
abstract::Energy homeostasis is controlled by a complex neuroendocrine system consisting of peripheral signals like leptin and central signals, in particular, neuropeptides. Several neuropeptides with anorexigenic (POMC, CART, and CRH) as well as orexigenic (NPY, AgRP, and MCH) actions are involved in this complex (partly redun...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(02)00269-3
更新日期:2002-12-01 00:00:00
abstract::The effects of atrial natriuretic factor (ANF) on [3H]norepinephrine ([3H]NE) release evoked by a sodium-free medium (SFM) were studied. Experiments were performed in rat adrenal medulla slices incubated in vitro. Results showed that [3H]NE release evoked by the omission of Na+ was decreased by 10 nM ANF. In addition,...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90100-7
更新日期:1994-01-01 00:00:00
abstract::Peptide YY (PYY)(3-36), a neuropeptide Y (NPY) Y2 receptor agonist, is a powerful inhibitor of intestinal secretion. Based on this anti-secretory effect, NPY Y2 receptor agonists may be useful as novel anti-diarrheal agents, but anti-diarrheal efficacy has yet to be determined. We therefore examined the anti-diarrheal...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.11.005
更新日期:2010-04-01 00:00:00
abstract::The presence of immunoreactive endothelin (IR-ET) in human saliva and rat parotid gland was investigated by radioimmunoassay. The IR-ET concentration (mean +/- SEM) in saliva taken from normal volunteers was 2.0 +/- 0.2 pmol/l (n = 15). The IR-ET concentration in rat parotid gland was 19.2 +/- 2.2 fmol/g wet weight (n...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90151-e
更新日期:1991-07-01 00:00:00
abstract::The biological activities of alpha-MSH des-acetyl MSH, gamma-MSH and LPH37-58 were compared using the Anolis rate method of bioassay. Dose-response data showed LPH37-58 to be equipotent with alpha-MSH, but des-acetyl MSH and gamma-MSH were found to be much less active. The effect of LPH37-58 was additive to that of al...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90134-6
更新日期:1982-01-01 00:00:00
abstract::As the frequent emergence of the resistant bacteria, the development of new agents with a new action mode attracts a great deal of interest. It is now widely accepted that antimicrobial peptides (AMPs) are promising alternatives to conventional antibiotics. In this study, antimicrobial peptide polybia-MPI and its anal...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.11.002
更新日期:2013-01-01 00:00:00
abstract::The expression of the relaxin-3 gene, detected as a new member of the insulin superfamily using human genomic databases, is abundantly present in the brain and testis. Intracerebroventricularly (icv) administered relaxin-3 stimulates food intake. Icv administered relaxin (identical to relaxin-2 in humans) affects the ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.02.020
更新日期:2010-06-01 00:00:00
abstract::Previously, we described a gene, flp-1, that encodes seven FMRFamide-like peptides from two alternatively spliced transcripts in the nematode Caenorhabditis elegans. To determine whether all or a subset of the predicted peptides coded for by flp-1 are produced in vivo, we undertook the isolation of FMRFamide-like pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90049-m
更新日期:1993-03-01 00:00:00
abstract::Intestinal levels of immunoreactive neurotensin (iNT) and neuromedin N (iNMN), as well as mRNA for the NT/NMN precursor, were elevated during the suckling period in rats. While transient expression of NT/NMN was observed at 1-5 days of age in the proximal small intestine and colon, NT/NMN levels in the ileum increased...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90161-9
更新日期:1993-11-01 00:00:00
abstract::Unilateral intracerebroventricular (ICV) injections of a high dose of CCK7 have been reported to elicit barrel rotations accompanied by contralateral postural asymmetry; there was no spontaneous locomotor activity other than barrel rolling. The aim of the present study was to investigate the effects of lower doses of ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90126-4
更新日期:1987-11-01 00:00:00
abstract::Intrahypothalamic injection of amylin (AMY) was shown to reduce the intake of rat chow and water for 8 and 4 h, respectively, in schedule-fed rats. Amylin also reduced water intake to a much lesser degree in 24-h water-deprived rats. A test of the ability of AMY to form a conditioned taste aversion yielded no change i...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90057-a
更新日期:1992-09-01 00:00:00
abstract::Kallikrein-related peptidases (KLKs) are a group of serine proteases, expressed in several tissues. Their activity is regulated by inhibitors including members of the serine protease of Kazal-type (SPINK) family. Recently, we discovered that SPINK6 is expressed in human skin and inhibits KLK5, KLK7, KLK14 but not KLK8...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.03.009
更新日期:2011-06-01 00:00:00
abstract::Dispersed cells from chicken brain and liver were found to possess cell surface binding sites for 125I-neurotensin (125I-NT). Scatchard analyses indicated the presence of high affinity (K4, 25-80 pM) and low affinity (Kd, 250-450 pM) components in adult tissues. Binding capacity was reduced 25-40% by incubation with p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00052-1
更新日期:1997-01-01 00:00:00
abstract::Double-labeling immunofluorescence of guinea pig tracheobronchial lymph nodes revealed complete coincidence of SP and CGRP immunoreactivities in perivascular nerves and axons of the medullary lymphatic tissue. Additional dynorphin A or cholecystokinin immunoreactivity was seen only in some of the medullary fibers. Ult...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90103-c
更新日期:1990-01-01 00:00:00
abstract::The cholecystokinin receptor-type 1 (CCK1R) is a G-protein coupled receptor localized in the animal gastrointestinal tract. Receptor activation by the natural peptide ligand CCK leads to a feeling of satiety. In this study, hydrolysates from soy and milk proteins were evaluated for their potential to activate the CCK1...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.11.019
更新日期:2012-03-01 00:00:00
abstract::Tardive dyskinesia, a clinical syndrome, is one of the major side effects of protracted treatment with neuroleptics in schizophrenic patients. Functional supersensitivity of striatal dopamine receptors is believed to contribute to the pathogenesis of schizophrenia and tardive dyskinesia. In a rodent model of neurolept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90072-6
更新日期:1987-09-01 00:00:00
abstract::Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain. Regional unidirectional transfer constants (Kin) calculated from the multiple-time brain uptake analysis were 0.93, 1.33 and 1.66 microliter.min-1 g-1 ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90026-0
更新日期:1989-03-01 00:00:00
abstract::The extractability of neurotensin (NT) from porcine ileal mucosa was studied by comparison of eight extraction procedures. Tissue content of neurotensin-like immunoreactivity was quantitated and characterized by sequence-specific radioimmunoassays and gel filtration chromatography. Homogenization prior to boiling in e...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90038-6
更新日期:1987-07-01 00:00:00
abstract::To identify a minimal peptide ligand that participates in recognition of heparin, we synthesized peptides extending a 29-kDa fragment in the C-terminal heparin binding domain of fibronectin. We obtained evidence that two peptides (designated D5 and D1) exhibit inhibitory activity on binding of the 29-kDa fragment to h...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00149-0
更新日期:1996-01-01 00:00:00
abstract::The synthesis is described of an analogue of the locust CRF-like diuretic peptide in which methionine in positions 1,3, and 13 is replaced by isosteric methyl-homoserine residues. This analogue has been tested for biological activity on Malpighian tubules in vitro, and feeding behavior in vivo. It is highly active in ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.09.010
更新日期:2003-10-01 00:00:00
abstract::The binding and internalization of a cytotoxic analogue of luteinizing hormone-releasing hormone (LH-RH), T-98 (agonist [D-Lys6]LH-RH linked to glutaryl-2-(hydroxymethyl)anthraquinone), by rat anterior pituitary cells was investigated. Analogue T-98 was bound to pituitary membrane binding sites for LH-RH with a high a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90024-8
更新日期:1994-01-01 00:00:00
abstract::Antimicrobial peptides (AMP) have been widely described in several organisms from different kingdoms. We recently designed and evaluated a synthetic version of an AMP isolated and characterized from Argopecten purpuratus hemocytes. This study describes the generation of a chimaeric gene encoding for Ap-S, the use of t...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.07.007
更新日期:2011-09-01 00:00:00
abstract::Intravenous (IV) infusions of neurotensin (NT) in anesthetized guinea pigs elicited dose-dependent pressor effects and tachycardia. Both effects were significantly reduced or abolished in guinea pigs given a chronic treatment with the neurotoxin capsaicin. In guinea pig isolated atria NT evoked a positive inotropic an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90140-9
更新日期:1987-11-01 00:00:00