Atypical mycobacterial infection in patients with cystic fibrosis: update on clinical microbiology methods.

abstract：:Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 2...

journal_title：Letters in applied microbiology

authors： Hudson JA,Nicol C,Capill J,Bennett J

更新日期：2000-02-01 00:00:00

abstract：AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (desig...

journal_title：Letters in applied microbiology

authors： Deshpande NM,Dhakephalkar PK,Kanekar PP

更新日期：2001-10-01 00:00:00

abstract：AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...

journal_title：Letters in applied microbiology

authors： Al Groosh D,Roudsari GB,Moles DR,Ready D,Noar JH,Pratten J

更新日期：2011-05-01 00:00:00

abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

journal_title：Letters in applied microbiology

authors： Park JP,Kim SW,Hwang HJ,Yun JW

更新日期：2001-07-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...

journal_title：Letters in applied microbiology

authors： Faisal M,Hasnain S

更新日期：2006-10-01 00:00:00

abstract：AIMS:To develop a colorimetric colony-screening assay to facilitate the isolation of micro-organisms capable of defluorination. METHODS AND RESULTS:A metal-dye chelate, zirconium-xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. Depolymerised zirconium ...

journal_title：Letters in applied microbiology

authors： Davis CK,Denman SE,Sly LI,McSweeney CS

更新日期：2011-10-01 00:00:00

abstract：AIMS:To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. METHODS AND RESULTS:A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transc...

journal_title：Letters in applied microbiology

authors： Namsai A,Louisirirotchanakul S,Wongchinda N,Siripanyaphinyo U,Virulhakul P,Puthavathana P,Myint KS,Gannarong M,Ittapong R

更新日期：2011-12-01 00:00:00

abstract：UNLABELLED:The structure and dynamics of microbial populations play a significant role during cheese manufacture and ripening. Therefore, fast and accurate methods for identification and characterization of the microbial populations are of fundamental importance to the cheese industry. In this study, we investigate the...

journal_title：Letters in applied microbiology

authors： Porcellato D,Brighton C,McMahon DJ,Oberg CJ,Lefevre M,Broadbent JR,Steele JL

更新日期：2014-08-01 00:00:00

abstract：UNLABELLED:The objective of this study was to evaluate the effect of different caffeine concentrations (0-4%) on (i) lag phase prior to growth, (ii) growth rates and (iii) ochratoxin A (OTA) production by strains from the Aspergillus section Circumdati and Aspergillus section Nigri groups, isolated from coffee, when gr...

journal_title：Letters in applied microbiology

authors： Akbar A,Medina A,Magan N

更新日期：2016-07-01 00:00:00

abstract：:Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...

journal_title：Letters in applied microbiology

authors： Erdem B,Threlfall EJ,Schofield SL,Ward LR,Rowe B

更新日期：1994-10-01 00:00:00

abstract：:Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

journal_title：Letters in applied microbiology

authors： Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

更新日期：2018-04-01 00:00:00

abstract：AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...

journal_title：Letters in applied microbiology

authors： Zhang HY,Yang Q,Wang G,Shang FD

更新日期：2009-03-01 00:00:00

abstract：:Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

journal_title：Letters in applied microbiology

authors： Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

更新日期：1997-10-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...

journal_title：Letters in applied microbiology

authors： Magalhães TF,da Silva CM,de Fátima Â,da Silva DL,Modolo LV,Martins CV,Alves RB,Ruiz AL,Longato GB,de Carvalho JE,de Resende-Stoianoff MA

更新日期：2013-08-01 00:00:00

abstract：:A pilot survey of the counts of total coliform bacteria, thermotolerant coliform bacteria, Escherichia coli and faecal streptococci was carried out at eight inland recreational waters at weekly intervals during July 1991. The aims were to assess the feasibility of determining candidate indicators of recreational water...

journal_title：Letters in applied microbiology

authors：

更新日期：1995-10-01 00:00:00

abstract：AIMS:The objective of this study is to actively express a novel fibrinolytic enzyme, subtilisin DFE (douchi fibrinolytic enzyme), in Escherichia coli. METHODS AND RESULTS:The DNA fragments encoding pro-subtilisin DFE was amplified and cloned into the vector pET32a to obtain N-terminal Trx fusion expression plasmid. Th...

journal_title：Letters in applied microbiology

authors： Zhang RH,Xiao L,Peng Y,Wang HY,Bai F,Zhang YZ

更新日期：2005-01-01 00:00:00

abstract：:Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity t...

journal_title：Letters in applied microbiology

authors： Mostafa ME

更新日期：1995-04-01 00:00:00

abstract：AIM:The main aim of this study was to investigate a possible connection between the Salmonella content in sewage sludge and human cases of salmonellosis. An additional aim was to survey the antimicrobial resistance situation in Salmonella isolated from Swedish sewage sludge. METHODS AND RESULTS:The Salmonella strains ...

journal_title：Letters in applied microbiology

authors： Sahlström L,de Jong B,Aspan A

更新日期：2006-07-01 00:00:00

abstract：:Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a si...

journal_title：Letters in applied microbiology

authors： Elvin S,Bingham AH

更新日期：1991-02-01 00:00:00

abstract：:The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal...

journal_title：Letters in applied microbiology

authors： Valík Ľ,Ačai P,Liptáková D

更新日期：2017-11-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...

journal_title：Letters in applied microbiology

authors： Tanaro JD,Pianciola LA,D'Astek BA,Piaggio MC,Mazzeo ML,Zolezzi G,Rivas M

更新日期：2018-06-01 00:00:00

abstract：AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

journal_title：Letters in applied microbiology

authors： Buddie AG,Bridge PD,Kelley J,Ryan MJ

更新日期：2011-01-01 00:00:00

abstract：:The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

journal_title：Letters in applied microbiology

authors： Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

更新日期：1998-06-01 00:00:00

abstract：:The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...

journal_title：Letters in applied microbiology

authors： Flahaut S,Laplace JM,Frère J,Auffray Y

更新日期：1998-04-01 00:00:00

abstract：UNLABELLED:Sandalwood oil has been found in numerous therapeutic applications in traditional medicines such as Chinese traditional medicine and Ayurveda. However, there are no comparative accounts available in the literature that focused on in vitro and in vivo tree sample-derived extracts. Combined dichloromethane and...

journal_title：Letters in applied microbiology

authors： Misra BB,Dey S

更新日期：2012-12-01 00:00:00

abstract：AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently an...

journal_title：Letters in applied microbiology

authors： Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GS

更新日期：2002-01-01 00:00:00

abstract：AIMS:To demonstrate that produce rinsates used for RT-qPCR detection of foodborne viruses may cause significant PCR inhibition and propose a means to reduce its impact on sensitivity. METHODS AND RESULTS:Here, it is shown that rinsing and concentration from spinach and precut lettuce have the potential to generate RNA...

journal_title：Letters in applied microbiology

authors： Plante D,Bélanger G,Leblanc D,Ward P,Houde A,Trottier YL

更新日期：2011-03-01 00:00:00

abstract：AIMS:Natural and synthetic antimicrobial peptides (AMPs) are of increasing interest as potential resistance conferring elements in plants against pathogen infection. The efficacy of AMPs against pathogens is prescreened by in vitro assays, and promising AMP candidates are introduced as transgenes into plants. As in vit...

journal_title：Letters in applied microbiology

authors： Visser M,Stephan D,Jaynes JM,Burger JT

更新日期：2012-06-01 00:00:00