Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris.

Abstract:

AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask culture were found to be 20 degrees C and 6.0, respectively. Sucrose (40 g x l(-1)) and corn steep powder (10 g x l(-1)) were the most suitable carbon and nitrogen source for both mycelial growth and exo-biopolymer production. CONCLUSION:Under optimal culture conditions, the maximum exo-biopolymer concentration in a 5-l jar fermenter indicated 10.3 g x l(-1), which was approximately three times higher than that in shake flask culture. SIGNIFICANCE AND IMPACT OF THE STUDY:This process can have a significant impact on the industrial scale when sucrose and corn steep powder were used as carbon and nitrogen source.

journal_name

Lett Appl Microbiol

authors

Park JP,Kim SW,Hwang HJ,Yun JW

doi

10.1046/j.1472-765x.2001.00950.x

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

76-81

issue

1

eissn

0266-8254

issn

1472-765X

pii

lam950

journal_volume

33

pub_type

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