Overexpression of Ecm22 improves ergosterol biosynthesis in Saccharomyces cerevisiae.

abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate the use of mannitol salt agar (MSA) supplemented with acriflavine for selective growth and quantification of Staphylococcus aureus from flushed dairy manure wastewater (FDMW). METHODS AND RESULTS:Minimal inhibitory concentrations of acriflavine in MSA were determined by comparing the growth of S. a...

journal_title：Letters in applied microbiology

authors： Davis JA,Farrah SR,Wilkie AC

更新日期：2006-06-01 00:00:00

abstract：:The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

journal_title：Letters in applied microbiology

authors： Park SH,Kim WJ,Kang DH

更新日期：2018-08-01 00:00:00

abstract：:A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic Europ...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Rasmussen OF

更新日期：1994-11-01 00:00:00

abstract：:Pyrimidine synthesis in the food spoilage agent Burkholderia cepacia ATCC 25416 was investigated. The five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in B. cepacia ATCC 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. The in vitro regulation of a...

journal_title：Letters in applied microbiology

authors： Li K,West TP

更新日期：1995-11-01 00:00:00

abstract：AIM:  This report describes the use of a six-gene multi-locus sequence analysis (MLSA) to correctly identify Vibrio strains of the Harveyi clade. METHODS AND RESULTS:  Vibrio isolates were characterized using a six housekeeping gene MLSA. The study provided evidence supporting: (i) a substantial number of reference st...

journal_title：Letters in applied microbiology

authors： Hoffmann M,Monday SR,Fischer M,Brown EW

更新日期：2012-02-01 00:00:00

abstract：:From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...

journal_title：Letters in applied microbiology

authors： Ahrens P,Friis NF

更新日期：1991-06-01 00:00:00

abstract：AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

journal_title：Letters in applied microbiology

authors： Asis CA Jr,Adachi K

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:Sandalwood oil has been found in numerous therapeutic applications in traditional medicines such as Chinese traditional medicine and Ayurveda. However, there are no comparative accounts available in the literature that focused on in vitro and in vivo tree sample-derived extracts. Combined dichloromethane and...

journal_title：Letters in applied microbiology

authors： Misra BB,Dey S

更新日期：2012-12-01 00:00:00

abstract：AIMS:To identify several strains of Mesorhizobium amorphae and Mesorhizobium tianshanense nodulating Cicer arietinum in Spain and Portugal, and to study the symbiotic genes carried by these strains. METHODS AND RESULTS:The sequences of 16S-23S intergenic spacer (ITS), 16S rRNA gene and symbiotic genes nodC and nifH we...

journal_title：Letters in applied microbiology

authors： Rivas R,Laranjo M,Mateos PF,Oliveira S,Martínez-Molina E,Velázquez E

更新日期：2007-04-01 00:00:00

abstract：:Investigations of a free-range broiler flock during the rearing period and at the slaughterhouse by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the flagellin (flaA) gene (flaA typing) have shown that poultry carcasses are contaminated by Campylobacter spp. strains which were previo...

journal_title：Letters in applied microbiology

authors： Rivoal K,Denis M,Salvat G,Colin P,Ermel G

更新日期：1999-12-01 00:00:00

abstract：AIMS:To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. METHODS AND RESULTS:A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transc...

journal_title：Letters in applied microbiology

authors： Namsai A,Louisirirotchanakul S,Wongchinda N,Siripanyaphinyo U,Virulhakul P,Puthavathana P,Myint KS,Gannarong M,Ittapong R

更新日期：2011-12-01 00:00:00

abstract：:RAPD technique (randomly amplified polymorphic DNA) was used for epidemiological subtyping of Bacillus licheniformis and other Bacillus spp. Within 46 isolates of B. licheniformis, up to 10 strain types could be determined when two 10-mer primers were used. RAPD patterns, which were found in eight further strains of B...

journal_title：Letters in applied microbiology

authors： Stephan R,Schraft H,Untermann F

更新日期：1994-05-01 00:00:00

abstract：AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...

journal_title：Letters in applied microbiology

authors： Schmidt H,Bannier M,Vogel RF,Niessen L

更新日期：2004-01-01 00:00:00

abstract：:The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal...

journal_title：Letters in applied microbiology

authors： Valík Ľ,Ačai P,Liptáková D

更新日期：2017-11-01 00:00:00

abstract：AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

journal_title：Letters in applied microbiology

authors： Sun J,Hu XL,Le GW,Shi YH

更新日期：2010-03-01 00:00:00

abstract：:An efficient method is described for the purification of Ti plasmid DNA from Agrobacterium. The procedure is based on the relative binding capacity of ethidium bromide to supercoiled plasmid DNA and linear DNA and on the high solubility of ethidium bromide in phenol. Following treatment with ethidium bromide, more tha...

journal_title：Letters in applied microbiology

authors： Zhang L,Kerr A

更新日期：1993-05-01 00:00:00

abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

journal_title：Letters in applied microbiology

authors： Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

更新日期：2004-01-01 00:00:00

abstract：:Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic spec...

journal_title：Letters in applied microbiology

authors： Pysova I,Tumova P,Tolarova V,Nohynkova E

更新日期：2009-04-01 00:00:00

abstract：AIMS:To investigate the influence of the growth phase, growth temperature, storage time, pH and aw of the treatment medium on the resistance of Listeria monocytogenes to pulsed electric fields (PEF). METHODS AND RESULTS:Square wave pulses of 2 micros at a frequency of 1 Hz and 25 and 28 kV cm(-1) were used. Cells were...

journal_title：Letters in applied microbiology

authors： Alvarez I,Pagán R,Raso J,Condón S

更新日期：2002-01-01 00:00:00

abstract：:Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

journal_title：Letters in applied microbiology

authors： Black SF,Gray DI,Fenlon DR,Kroll RG

更新日期：1995-03-01 00:00:00

abstract：:While the beta-glucuronidase activity of intact cells of Clostridium perfringens was higher in 0.95% sodium chloride (NaCl) than that in 0, 0.1 or 0.5%, that of Escherichia coli was higher in 0.1% NaCl than that in 0, 0.5 or 0.95% NaCl in 0.1 mol l-1 KH2PO4. However, the enzyme activity of both species of intact cells...

journal_title：Letters in applied microbiology

authors： Fujisawa T,Aikawa K,Takahashi T,Yamai S

更新日期：2000-09-01 00:00:00

abstract：:The use of energy dispersive analysis of X-rays (EDAX) to identify and quantify the presence of chlorhexidine diacetate (CHA) within Saccharomyces cerevisiae cells was examined. Chlorine was used as the elemental marker tag. Saccharomyces cerevisiae cells exposed to 1000 micrograms ml-1 CHA took up increasing amounts ...

journal_title：Letters in applied microbiology

authors： Hiom SJ,Hann AC,Furr JR,Russell AD

更新日期：1995-06-01 00:00:00

abstract：AIM:The main aim of this study was to investigate a possible connection between the Salmonella content in sewage sludge and human cases of salmonellosis. An additional aim was to survey the antimicrobial resistance situation in Salmonella isolated from Swedish sewage sludge. METHODS AND RESULTS:The Salmonella strains ...

journal_title：Letters in applied microbiology

authors： Sahlström L,de Jong B,Aspan A

更新日期：2006-07-01 00:00:00

abstract：AIMS:In traditional Thai medicine, nutgall of Quercus infectoria G. Olivier is well-documented as an effective agent for wound and skin infections. The present study was aimed to establish modes of action of the ethanol extract of the plant as well as its main constituents to induce anti-methicillin-resistant Staphyloc...

journal_title：Letters in applied microbiology

authors： Chusri S,Voravuthikunchai SP

更新日期：2011-06-01 00:00:00

abstract：AIMS:To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. METHODS AND RESULTS:Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was s...

journal_title：Letters in applied microbiology

authors： de la Fuente G,Cebrián JA,Fondevila M

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:Several serological diagnostics rely on enzyme-linked immunosorbent assay (ELISA) to detect bacterial infections. However, for some pathogens, including Bartonella henselae, diagnosis still depends on manually intensive, time-consuming assays including micro-immunofluorescence, Western blotting or indirect i...

journal_title：Letters in applied microbiology

authors： Ferrara F,Di Niro R,D'Angelo S,Busetti M,Marzari R,Not T,Sblattero D

更新日期：2014-09-01 00:00:00

abstract：:The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...

journal_title：Letters in applied microbiology

authors： Matteuzzi D,Brigidi P,Rossi M,Di D

更新日期：1990-10-01 00:00:00

abstract：AIMS:To develop a colorimetric colony-screening assay to facilitate the isolation of micro-organisms capable of defluorination. METHODS AND RESULTS:A metal-dye chelate, zirconium-xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. Depolymerised zirconium ...

journal_title：Letters in applied microbiology

authors： Davis CK,Denman SE,Sly LI,McSweeney CS

更新日期：2011-10-01 00:00:00

abstract：:Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...

journal_title：Letters in applied microbiology

authors： Fenlon DR,Stewart T,Donachie W

更新日期：1995-01-01 00:00:00