Influence of sodium chloride on the beta-glucuronidase activity of Clostridium perfringens and Escherichia coli.

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：:The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...

journal_title：Letters in applied microbiology

authors： Flahaut S,Laplace JM,Frère J,Auffray Y

更新日期：1998-04-01 00:00:00

abstract：AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...

journal_title：Letters in applied microbiology

authors： Berlec A,Tompa G,Slapar N,Fonović UP,Rogelj I,Strukelj B

更新日期：2008-02-01 00:00:00

abstract：AIM:The biotransformation of pentoxifylline (PTX), propentofylline (PPT) and their racemic hydroxy metabolites ((+/-)-OHPTX and (+/-)-OHPPT) by using the fungus Cunninghamella echinulata NRRL 1384. METHODS AND RESULTS:A fungus Cunninghamella echinulata NRRL 1384 was used to catalyse the (S)-selective oxidation of the ...

journal_title：Letters in applied microbiology

authors： Pekala E,Kochan M,Carnell AJ

更新日期：2009-01-01 00:00:00

abstract：AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...

journal_title：Letters in applied microbiology

authors： Sparo MD,Jones DG,Sánchez Bruni SF

更新日期：2009-02-01 00:00:00

abstract：AIMS:Haloarchaeal proteases function optimally in high salt (low water activity); thus, they offer an advantage over the nonhalophilic counterparts as biocatalysts for protease-catalysed peptide synthesis. The haloalkaliphilic archaeon Natrialba magadii secretes a solvent-tolerant protease, Nep (Natrialba magadii extra...

journal_title：Letters in applied microbiology

authors： Ruiz DM,Iannuci NB,Cascone O,De Castro RE

更新日期：2010-12-01 00:00:00

abstract：AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

journal_title：Letters in applied microbiology

authors： Rogers JV,Ducatte GR,Choi YW,Early PC

更新日期：2006-11-01 00:00:00

abstract：:In order to evaluate possible indicators of viral aerosol contamination in sewage treatment plants, a year-long study was carried out on the relationships between the presence of cytopathogenic viruses and the counts of total bacteria, faecal streptococci and somatic coliphages in samples collected at various distance...

journal_title：Letters in applied microbiology

authors： Carducci A,Gemelli C,Cantiani L,Casini B,Rovini E

更新日期：1999-03-01 00:00:00

abstract：:White Spot Syndrome Virus (WSSV) is a dsDNA virus causing White Spot Syndrome Disease (WSSD) in shrimp with almost 100% morality rate within 3-10 days. In Bangladesh, WSSD is one of the major impediments of shrimp farming. This study first investigated the prevalence and distribution of WSSV in cultured shrimps of the...

journal_title：Letters in applied microbiology

authors： Hossain A,Nandi SP,Siddique MA,Sanyal SK,Sultana M,Hossain MA

更新日期：2015-02-01 00:00:00

abstract：AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To...

journal_title：Letters in applied microbiology

authors： Yin Y,Liu X,Ma Z

更新日期：2009-06-01 00:00:00

abstract：AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

journal_title：Letters in applied microbiology

authors： Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

更新日期：2008-05-01 00:00:00

abstract：AIMS:The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied. METHODS AND RESULTS:Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 k...

journal_title：Letters in applied microbiology

authors： Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

更新日期：2002-01-01 00:00:00

abstract：AIMS:Food-borne pathogen inhibition was tested in the presence of a mixture of Lactobacillus acidophilus and Lactobacillus casei during fermentation under controlled pH conditions. METHODS AND RESULTS:The growth of Escherichia coli O157:H7, Salmonella serotype Typhimurium, Staphylococcus aureus, Listeria innocua, Ente...

journal_title：Letters in applied microbiology

authors： Millette M,Luquet FM,Lacroix M

更新日期：2007-03-01 00:00:00

abstract：UNLABELLED:Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species ...

journal_title：Letters in applied microbiology

authors： Dehaut A,Midelet-Bourdin G,Brisabois A,Duflos G

更新日期：2014-11-01 00:00:00

abstract：:Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercia...

journal_title：Letters in applied microbiology

authors： Hilton AC,Banks JG,Penn CW

更新日期：1997-04-01 00:00:00

abstract：:A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. je...

journal_title：Letters in applied microbiology

authors： Mazurier S,van de Giessen A,Heuvelman K,Wernars K

更新日期：1992-06-01 00:00:00

abstract：AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...

journal_title：Letters in applied microbiology

authors： Rogers JV,Richter WR,Shaw MQ,Choi YW

更新日期：2008-10-01 00:00:00

abstract：:Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic spec...

journal_title：Letters in applied microbiology

authors： Pysova I,Tumova P,Tolarova V,Nohynkova E

更新日期：2009-04-01 00:00:00

abstract：UNLABELLED:The number of naphtha plants is being reduced due to a worldwide shift in energy sources. Consequently, a shortage of chemical materials heavily dependent on naphtha-oil, especially C4 compounds such as butene and butane-diol, is an urgent issue in chemical manufacturing. Erythritol is a rare C4 compound pro...

journal_title：Letters in applied microbiology

authors： Kobayashi Y,Iwata H,Mizushima D,Ogihara J,Kasumi T

更新日期：2015-05-01 00:00:00

abstract：:Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

journal_title：Letters in applied microbiology

authors： Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

更新日期：2018-04-01 00:00:00

abstract：AIMS:To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. METHODS AND RESULTS:The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons we...

journal_title：Letters in applied microbiology

authors： Wang HY,Gao YB,Fan QW,Xu Y

更新日期：2011-08-01 00:00:00

abstract：AIMS:To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. METHODS AND RESULTS:Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was s...

journal_title：Letters in applied microbiology

authors： de la Fuente G,Cebrián JA,Fondevila M

更新日期：2004-01-01 00:00:00

abstract：AIM:To identify the gene that encodes nigrescin, a bacteriocin produced by Prevotella nigrescens ATCC 25261. METHODS AND RESULTS:Each open reading frame (ORF) of the nig gene cluster (nigA, nigB, nigC and nigD) was transferred into an expression vector. The recombinant proteins encoded by nigA, nigB, nigC and nigD wer...

journal_title：Letters in applied microbiology

authors： Teanpaisan R,Narawatthana S,Utarabhand P

更新日期：2009-09-01 00:00:00

abstract：:Three white rot fungi were compared for their ability to attack polychlorinated biphenyl (PCB) congeners in the presence and absence of the non-ionic Triton X-100 or the anionic Dowfax 8390 surfactants at half their critical micelle concentrations. Neither surfactant affected PCB biodegradation monitored by gas chroma...

journal_title：Letters in applied microbiology

authors： Beaudette LA,Ward OP,Pickard MA,Fedorak PM

更新日期：2000-02-01 00:00:00

abstract：AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...

journal_title：Letters in applied microbiology

authors： Li Q,Sherwood JS,Logue CM

更新日期：2007-01-01 00:00:00

abstract：AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...

journal_title：Letters in applied microbiology

authors： Duarte RT,Staats CC,Fungaro MH,Schrank A,Vainsten MH,Furlaneto-Maia L,Nakamura CV,de Souza W,Furlaneto MC

更新日期：2007-03-01 00:00:00

abstract：UNLABELLED:Although little evidence existed to support that view, European countries and in particular France, have regarded echinoderms, including sea urchins, as low risk in terms of feacal contamination. It is hypothesized that the sea urchins mode of feeding, which is based on grazing and differs from bivalve mollu...

journal_title：Letters in applied microbiology

authors： Bouchoucha M,Piquet JC,Chavanon F,Dufresne C,Le Guyader FS

更新日期：2016-02-01 00:00:00

abstract：AIMS:A simplified and rapid method was developed to identify and enumerate total mesophilic microbial load, gram-negative bacteria, coliforms, and Escherichia coli in ground beef, using a single module. METHODS AND RESULTS:A 96-well microtiter plate was divided into four sections of two lanes (24 wells), with each sec...

journal_title：Letters in applied microbiology

authors： Kang DH,Rhee MS,Costello M

更新日期：2003-01-01 00:00:00

abstract：:Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of ...

journal_title：Letters in applied microbiology

authors： Abdel-Kareem MM,Zohri AA

更新日期：2018-11-01 00:00:00

abstract：AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...

journal_title：Letters in applied microbiology

authors： Lopes M,Gomes N,Gonçalves C,Coelho MA,Mota M,Belo I

更新日期：2008-02-01 00:00:00