A cryopreservation procedure for the rumen protozoon Entodinium caudatum: estimation of its viability by fluorescence microscopy.

abstract：AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...

journal_title：Letters in applied microbiology

authors： Rodríguez E,Arqués JL,Rodríguez R,Nuñez M,Medina M

更新日期：2003-01-01 00:00:00

abstract：AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

journal_title：Letters in applied microbiology

authors： Ottoson J,Norström A,Dalhammar G

更新日期：2005-01-01 00:00:00

abstract：AIMS:Bioprocess oxidative stress caused by many reactive oxygen species (ROS) can lead to largely irreversible perturbation of yeast bioprocesses. These include the production of proteins derived from recombinant DNA yeast technology (aerobically grown Saccharomyces cerevisiae). These proteins include rennin, amylogluc...

journal_title：Letters in applied microbiology

authors： Wiseman A

更新日期：2005-01-01 00:00:00

abstract：AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

journal_title：Letters in applied microbiology

authors： Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

更新日期：2007-09-01 00:00:00

abstract：AIMS:To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina. METHODS AND RESULTS:Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production...

journal_title：Letters in applied microbiology

authors： Xian M,Nie J,Meng Q,Liu J,Zhou C,Kang Y,Zhen K

更新日期：2003-01-01 00:00:00

abstract：AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...

journal_title：Letters in applied microbiology

authors： Lopes M,Gomes N,Gonçalves C,Coelho MA,Mota M,Belo I

更新日期：2008-02-01 00:00:00

abstract：AIMS:The aim of this study is to develop an RT-PCR assay combined with immunomagnetic beads (IMS/RT-PCR) coating monoclonal antibody (Mab) for separation and detection of norovirus (genogroup II) in faecal samples. We furthermore compare its detection limits with IMS/RT-PCR using polyclonal antibody (Pab) and the TRIzo...

journal_title：Letters in applied microbiology

authors： Yao L,Wu Q,Wang D,Kou X,Zhang J

更新日期：2009-08-01 00:00:00

abstract：AIMS:The study was aimed to evaluate the Mycobacterium leprae recombinant early secreted antigenic target-6 (rESAT-6) for its serological performance in leprosy patients. METHODS AND RESULTS:Employing enzyme-linked immunosorbent assay (ELISA), serum samples were tested for prevalence of immunoglobulin G antibodies aga...

journal_title：Letters in applied microbiology

authors： Parkash O,Pandey R,Kumar A,Kumar A

更新日期：2007-05-01 00:00:00

abstract：:The contribution of trehalose and hsp104 to barotolerance in Saccharomyces cerevisiae has been investigated. Mutant strains, which lacked the ability to accumulate trehalose and/or hsp104, were examined for barotolerance and thermotolerance. All the mutants showed lower barotolerance and thermotolerance than their con...

journal_title：Letters in applied microbiology

authors： Iwahashi H,Obuchi K,Fujii S,Komatsu Y

更新日期：1997-07-01 00:00:00

abstract：AIMS:To determine the underlying substrate utilization mechanism in the logistic equation for batch microbial growth by revealing the relationship between the logistic and Monod kinetics. Also, to determine the logistic rate constant in terms of Monod kinetic constants. METHODS AND RESULTS:The logistic equation used t...

journal_title：Letters in applied microbiology

authors： Kargi F

更新日期：2009-04-01 00:00:00

abstract：:A method for the diagnosis of mycobacterial infections by PCR amplification followed by selective restriction enzyme digestion of the PCR product was developed. The amplified DNA sequence used in this study occurs within the gene encoding for the mycobacterial 65 kDa heat shock protein (Hance et al. 1989), which is fo...

journal_title：Letters in applied microbiology

authors： Rodrigo G,Källenius G,Hoffmann E,Svenson SB

更新日期：1992-08-01 00:00:00

abstract：:A wild type micro-organism producing antibacterial substances has been isolated from a Chinese fermented soybean seasoning and identified as Bacillus subtilis. A crude antibacterial preparation (CABP) was obtained by ammonium sulphate precipitation. Isoelectric focusing assay revealed at least four antimicrobial compo...

journal_title：Letters in applied microbiology

authors： Zheng G,Slavik MF

更新日期：1999-05-01 00:00:00

abstract：AIM:The main aim of this study was to investigate a possible connection between the Salmonella content in sewage sludge and human cases of salmonellosis. An additional aim was to survey the antimicrobial resistance situation in Salmonella isolated from Swedish sewage sludge. METHODS AND RESULTS:The Salmonella strains ...

journal_title：Letters in applied microbiology

authors： Sahlström L,de Jong B,Aspan A

更新日期：2006-07-01 00:00:00

abstract：:Accumulation of proline and glycine betaine occurs via three transport systems in Staphylococcus aureus (Pourkomailian and Booth 1992, 1994). A 35 kDa membrane protein was identified to be missing from a mutant, BP108, which lacks activity of the low affinity osmolyte transport system. The loss in activity of the low ...

journal_title：Letters in applied microbiology

authors： Pourkomaillan B

更新日期：1998-02-01 00:00:00

abstract：UNLABELLED:This work aimed to study the interaction between Actinomycetal isolates and Aspergillus flavus to promote mutual antagonism in contact. Thirty-seven soilborn Streptomyces spp. isolates were chosen as potential candidates. After a 10-day in vitro co-incubation period, 27 isolates respond to the criteria, that...

journal_title：Letters in applied microbiology

authors： Verheecke C,Liboz T,Darriet M,Sabaou N,Mathieu F

更新日期：2014-06-01 00:00:00

abstract：AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...

journal_title：Letters in applied microbiology

authors： Harrison WA,Griffith CJ,Tennant D,Peters AC

更新日期：2001-12-01 00:00:00

abstract：:In this study, a total of 172 putative omega-3 producers were isolated from 28 sediment samples from the Arabian Gulf employing a selective isolation procedure using marine agar containing 0·1% triphenyl tetrazolium chloride (TTC). Out of these 172 isolates, 19 isolates produced eicosapentaenoic acid (EPA) as confirme...

journal_title：Letters in applied microbiology

authors： Alagarsamy S,Sabeena Farvin KH,Fakhraldeen S,Kooramattom MR,Al-Yamani F

更新日期：2019-08-01 00:00:00

abstract：AIM:Combination of immunomagnetic separation (IMS) and lateral flow device (LFD) assays for the development of a sensitive, rapid, on-site methodology that enables concentration and detection of Bacillus anthracis spores in complex samples. METHODS AND RESULTS:The data presents the development of an optimized, 30 min,...

journal_title：Letters in applied microbiology

authors： Fisher M,Atiya-Nasagi Y,Simon I,Gordin M,Mechaly A,Yitzhaki S

更新日期：2009-04-01 00:00:00

abstract：UNLABELLED:Fast microbial identification is becoming increasingly necessary in industry to improve microbial control and reduce biocide consumption. We compared the performances of two systems based on MALDI-TOF MS (VITEK MS and BIOTYPER) and two based on biochemical testing (BIOLOG, VITEK 2 Compact) with genetic metho...

journal_title：Letters in applied microbiology

authors： Urwyler SK,Glaubitz J

更新日期：2016-02-01 00:00:00

abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...

journal_title：Letters in applied microbiology

authors： Fitzgerald AC,Edrington TS,Looper ML,Callaway TR,Genovese KJ,Bischoff KM,McReynolds JL,Thomas JD,Anderson RC,Nisbet DJ

更新日期：2003-01-01 00:00:00

abstract：:Seventeen aerobic bacterial strains known to degrade one or more aromatic compounds were tested for their ability to utilize benzene, seven different methylated benzenes or other aromatic compounds as their sole source of carbon. The results are discussed with respect to the possibility of finding strains which degrad...

journal_title：Letters in applied microbiology

authors： Lang E

更新日期：1996-10-01 00:00:00

abstract：:Parabens have been widely used as antimicrobial preservatives in cosmetics, pharmaceuticals, foods and beverages. Commonly, methyl-, ethyl-, propyl- and butylparaben are used independently or in combination to maintain the quality of industrial products, and they are considered to have low toxicity. However, recent ev...

journal_title：Letters in applied microbiology

authors： Murata W,Yamaguchi Y,Fujita KI,Yamauchi K,Tanaka T,Ogita A

更新日期：2019-12-01 00:00:00

abstract：:Erwinia amylovora bacteria cause fire blight disease, which affects apple and pear production worldwide. The Erw. amylovora pyrC gene encodes a predicted dihydroorotase enzyme involved in pyrimidine biosynthesis. Here, we discovered that the Erw. amylovora pyrC244::Tn5 mutant was a uracil auxotroph. Unexpectedly, the ...

journal_title：Letters in applied microbiology

authors： Ramos LS,Sinn JP,Lehman BL,Pfeufer EE,Peter KA,McNellis TW

更新日期：2015-06-01 00:00:00

abstract：:Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic spec...

journal_title：Letters in applied microbiology

authors： Pysova I,Tumova P,Tolarova V,Nohynkova E

更新日期：2009-04-01 00:00:00

abstract：UNLABELLED:The structure and dynamics of microbial populations play a significant role during cheese manufacture and ripening. Therefore, fast and accurate methods for identification and characterization of the microbial populations are of fundamental importance to the cheese industry. In this study, we investigate the...

journal_title：Letters in applied microbiology

authors： Porcellato D,Brighton C,McMahon DJ,Oberg CJ,Lefevre M,Broadbent JR,Steele JL

更新日期：2014-08-01 00:00:00

abstract：AIMS:The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants. METHODS AND RESULTS:The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recomb...

journal_title：Letters in applied microbiology

authors： Alberto F,Navarro D,de Vries RP,Asther M,Record E

更新日期：2009-08-01 00:00:00

abstract：AIMS:To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. METHODS AND RESULTS:Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactob...

journal_title：Letters in applied microbiology

authors： Lebeer S,Verhoeven TL,Claes IJ,De Hertogh G,Vermeire S,Buyse J,Van Immerseel F,Vanderleyden J,De Keersmaecker SC

更新日期：2011-03-01 00:00:00

abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

journal_title：Letters in applied microbiology

authors： McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

更新日期：2005-01-01 00:00:00

abstract：:This study examines the diversity of streptomycetes causing potato scab using phenotypic identification, 16S rRNA sequence, and pathogenicity measurements. Pathogenicity did not correlate well with taxonomic identification, nor with phylogenetic position. This result suggests that neither phenotypic nor 16S characteri...

journal_title：Letters in applied microbiology

authors： Bramwell PA,Wiener P,Akkermans AD,Wellington EM

更新日期：1998-11-01 00:00:00

abstract：AIMS:To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. METHODS AND RESULTS:Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory...

journal_title：Letters in applied microbiology

authors： Henriques IS,Fonseca F,Alves A,Saavedra MJ,Correia A

更新日期：2008-12-01 00:00:00