A combined immunomagnetic separation and lateral flow method for a sensitive on-site detection of Bacillus anthracis spores--assessment in water and dairy products.

abstract：:Thirty-eight isolates of Hafnia alvei were characterized by biochemical profiles, ribotyping and pulsed-field gel electrophoresis (PFGE) patterns. The isolates were recovered from chub-packed (19 isolates) or retail (nine isolates) ground beef, or were obtained from culture repositories (10 isolates). Biochemical prof...

journal_title：Letters in applied microbiology

authors： Gamage SD,Luchansky JB,Ingham SC

更新日期：1998-02-01 00:00:00

abstract：UNLABELLED:Escherichia coli is part of the normal microflora of the intestines of mammals. However, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially Shiga toxigenic E. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome ...

journal_title：Letters in applied microbiology

authors： Ribeiro LF,Barbosa MM,de Rezende Pinto F,Guariz CS,Maluta RP,Rossi JR,Rossi GA,Lemos MV,do Amaral LA

更新日期：2016-03-01 00:00:00

abstract：:The growth of pure cultures of Clostridium perfringens (ATCC 12922) and Cl. sporogenes (PA 3679) in five non-selective media, fluid thioglycollate medium (FTM), rapid perfringens medium (RPM), Columbia broth Malthus (CBM), reinforced clostridial medium (RCM) and lactose sulphite (LS), was monitored using conductance m...

journal_title：Letters in applied microbiology

authors： Dromigny E,Bourrion F,Rugraf Y,Bolton FJ,Leden N

更新日期：1997-01-01 00:00:00

abstract：AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...

journal_title：Letters in applied microbiology

authors： Morel F,Delmas F,Jobin MP,Diviès C,Guzzo J

更新日期：2001-08-01 00:00:00

abstract：:For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain...

journal_title：Letters in applied microbiology

authors： Yang M,Mu T,Zhong W,Olajuyin AM,Xing J

更新日期：2017-08-01 00:00:00

abstract：AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...

journal_title：Letters in applied microbiology

authors： Myers JM,Myers CR

更新日期：2003-01-01 00:00:00

abstract：AIMS:To develop an intergeneric conjugation system for rimocidin-producing Streptomyces rimosus. METHODS AND RESULTS:High efficiencies of conjugation [10(-2)-10(-3) transconjugants/recipient colony forming units (CFU)] were obtained when spores of S. rimosus were heat treated at 40 degrees C for 10 min prior to mixing...

journal_title：Letters in applied microbiology

authors： Phornphisutthimas S,Sudtachat N,Bunyoo C,Chotewutmontri P,Panijpan B,Thamchaipenet A

更新日期：2010-05-01 00:00:00

abstract：:The contribution of trehalose and hsp104 to barotolerance in Saccharomyces cerevisiae has been investigated. Mutant strains, which lacked the ability to accumulate trehalose and/or hsp104, were examined for barotolerance and thermotolerance. All the mutants showed lower barotolerance and thermotolerance than their con...

journal_title：Letters in applied microbiology

authors： Iwahashi H,Obuchi K,Fujii S,Komatsu Y

更新日期：1997-07-01 00:00:00

abstract：AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...

journal_title：Letters in applied microbiology

authors： Arshad M,Nazli ZH,Khalid A,Zahir ZA

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues ...

journal_title：Letters in applied microbiology

authors： Elamri DE,Aouni M,Parnaudeau S,Le Guyader FS

更新日期：2006-10-01 00:00:00

abstract：UNLABELLED:We investigated the role of the Bradyrhizobium japonicum lpcC gene, encoding a mannosyl transferase, involved in the lipopolysaccharide (LPS) biosynthesis. The inactivation of the lpcC gene considerably altered the LPS structure and the cell surface properties. LPS analysis showed that the lpcC mutant JS715 ...

journal_title：Letters in applied microbiology

authors： Lee HI,In YH,Jeong SY,Jeon JM,Noh JG,So JS,Chang WS

更新日期：2014-07-01 00:00:00

abstract：:The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...

journal_title：Letters in applied microbiology

authors： Quang Le H,Suffredini E,Tien Pham D,Kim To A,De Medici D

更新日期：2018-11-01 00:00:00

abstract：:The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

journal_title：Letters in applied microbiology

authors： Park SH,Kim WJ,Kang DH

更新日期：2018-08-01 00:00:00

abstract：UNLABELLED:The structure and dynamics of microbial populations play a significant role during cheese manufacture and ripening. Therefore, fast and accurate methods for identification and characterization of the microbial populations are of fundamental importance to the cheese industry. In this study, we investigate the...

journal_title：Letters in applied microbiology

authors： Porcellato D,Brighton C,McMahon DJ,Oberg CJ,Lefevre M,Broadbent JR,Steele JL

更新日期：2014-08-01 00:00:00

abstract：:Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a si...

journal_title：Letters in applied microbiology

authors： Elvin S,Bingham AH

更新日期：1991-02-01 00:00:00

abstract：:To enhance the overall expression level of lipase isozymes which catalyse the same reaction in Pichia pastoris through co-expression of isozymes from different sources; several types of co-expression ways were constructed to determine the co-expression efficiencies of lipase isozymes in P. pastoris. The results showed...

journal_title：Letters in applied microbiology

authors： Cai H,Zhang T,Zhao M,Mao J,Cai C,Feng F

更新日期：2017-10-01 00:00:00

abstract：:Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. cavi...

journal_title：Letters in applied microbiology

authors： Hossain S,Wickramanayake MVKS,Dahanayake PS,Heo GJ

更新日期：2020-03-01 00:00:00

abstract：AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...

journal_title：Letters in applied microbiology

authors： Zhang HY,Yang Q,Wang G,Shang FD

更新日期：2009-03-01 00:00:00

abstract：AIMS:  The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS:  A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the ...

journal_title：Letters in applied microbiology

authors： Ruiz LM,Castro M,Barriga A,Jerez CA,Guiliani N

更新日期：2012-02-01 00:00:00

abstract：AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...

journal_title：Letters in applied microbiology

authors： Hansen CR,Khatiwara A,Ziprin R,Kwon YM

更新日期：2007-12-01 00:00:00

abstract：AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...

journal_title：Letters in applied microbiology

authors： Sparo MD,Jones DG,Sánchez Bruni SF

更新日期：2009-02-01 00:00:00

abstract：AIMS:To screen a pair of primers suitable for denaturing gradient gel electrophoretic (DGGE) analysis of ruminal methanogenic Archaea and to detect the archaeal communities in the rumen of goat. METHODS AND RESULTS:Nine primer pairs for 16S rDNA of methanogenic Archaea, including six for directed polymerase chain reac...

journal_title：Letters in applied microbiology

authors： Cheng YF,Mao SY,Liu JX,Zhu WY

更新日期：2009-05-01 00:00:00

abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...

journal_title：Letters in applied microbiology

authors： Korem M,Goldberg NS,Cahan A,Cohen MJ,Nissenbaum I,Moses AE

更新日期：2018-07-01 00:00:00

abstract：AIMS:This study was performed to investigate the microbiological contamination of digestate product (DP) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. METHODS AND RESULTS:Microbiological analyses were performed on bovine manure, fresh DP, liquid and solid fractions and stored ...

journal_title：Letters in applied microbiology

authors： Bonetta S,Ferretti E,Bonetta S,Fezia G,Carraro E

更新日期：2011-11-01 00:00:00

abstract：:Accumulation of proline and glycine betaine occurs via three transport systems in Staphylococcus aureus (Pourkomailian and Booth 1992, 1994). A 35 kDa membrane protein was identified to be missing from a mutant, BP108, which lacks activity of the low affinity osmolyte transport system. The loss in activity of the low ...

journal_title：Letters in applied microbiology

authors： Pourkomaillan B

更新日期：1998-02-01 00:00:00

abstract：:Staphylococcus epidermidis and Staphylococcus aureus, two bacterial strains commonly associated with biofilm-related medical infections and food poisoning, can rapidly colonize biotic and abiotic surfaces. The present study investigates the ability of anodic alumina surfaces with nanoporous surface topography to minim...

journal_title：Letters in applied microbiology

authors： Feng G,Cheng Y,Worobo RW,Borca-Tasciuc DA,Moraru CI

更新日期：2019-10-01 00:00:00

abstract：:Three white rot fungi were compared for their ability to attack polychlorinated biphenyl (PCB) congeners in the presence and absence of the non-ionic Triton X-100 or the anionic Dowfax 8390 surfactants at half their critical micelle concentrations. Neither surfactant affected PCB biodegradation monitored by gas chroma...

journal_title：Letters in applied microbiology

authors： Beaudette LA,Ward OP,Pickard MA,Fedorak PM

更新日期：2000-02-01 00:00:00

abstract：:A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...

journal_title：Letters in applied microbiology

authors： Liu TT,Ye FC,Pang CP,Yong TQ,Tang WD,Xiao J,Shang CH,Lu ZJ

更新日期：2020-09-01 00:00:00

abstract：AIM:To test the efficacy of a chemical (chlorous acid) for reducing the numbers of viable Alicyclobacillus acidoterrestris spores in laboratory media and on apples. METHODS AND RESULTS:Alicyclobacillus acidoterrestris spores in aqueous suspension and on apple surfaces of four different cultivars were treated with 268 ...

journal_title：Letters in applied microbiology

authors： Lee SY,Ryu SR,Kang DH

更新日期：2010-08-01 00:00:00

abstract：:Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production...

journal_title：Letters in applied microbiology

authors： Tang B,Zhao YC,Shi XM,Xu HY,Zhao YY,Dai CC,Liu FQ

更新日期：2018-05-01 00:00:00