In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle.


AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 different mastitic dairy cattle, sourced from two dairies in Tandil-Argentina. Isolates from each of the bacterial species screened which developed the lowest inhibition zones in response to the peptide, were further evaluated in a series of time-killing curve studies. No survivors were detected in whole strains (from the three Streptococcal species isolated) within 120 min of incubation in presence of the peptide. The Staph. aureus isolates were less sensitive but, nevertheless, a drop in viable counts to below the detection limit was achieved for all the test strains by the final postincubation sampling point at 180 min. CONCLUSIONS:The study demonstrated the in vitro efficacy of the AP-CECT7121 against a variety strains of Gram positives isolated from mastitic dairy cattle. SIGNIFICANCE AND IMPACT OF THE STUDY:There is urgent global interest in the development of natural alternatives for the control and prevention of mastitis. Confirmation of the in vitro activity of the novel AP-CECT7121 against Gram-positive isolates encourages further research.


Lett Appl Microbiol


Sparo MD,Jones DG,Sánchez Bruni SF




Has Abstract


2009-02-01 00:00:00














  • Electronic paramagnetic resonance investigation of the activity of Origanum vulgare L. essential oil on the Listeria monocytogenes membrane.

    abstract:AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Serio A,Chiarini M,Tettamanti E,Paparella A

    更新日期:2010-08-01 00:00:00

  • Rapid and sensitive detection of Vibrio cholerae by loop-mediated isothermal amplification targeted to the gene of outer membrane protein ompW.

    abstract:AIMS:The present study was aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Vibrio cholerae. METHODS AND RESULTS:A set of five designed primers that recognized specifically the V. cholerae ompW gene was used. The optimized time and temperature conditions for th...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Srisuk C,Chaivisuthangkura P,Rukpratanporn S,Longyant S,Sridulyakul P,Sithigorngul P

    更新日期:2010-01-01 00:00:00

  • Rapid identification of Lactobacillus brevis using the polymerase chain reaction.

    abstract:AIMS:Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. METHODS AND RESULTS:Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplif...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Guarneri T,Rossetti L,Giraffa G

    更新日期:2001-11-01 00:00:00

  • Some potential sources for transmission of Campylobacter jejuni to broiler chickens.

    abstract:AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Ramabu SS,Boxall NS,Madie P,Fenwick SG

    更新日期:2004-01-01 00:00:00

  • Detection and characterization of the ferric uptake regulator (fur) gene in Plesiomonas shigelloides.

    abstract::Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Rodríguez-Rodríguez S,Santos JA

    更新日期:2018-04-01 00:00:00

  • Technical advance in fungal biotechnology: development of a miniaturized culture method and an automated high-throughput screening.

    abstract:AIMS:The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants. METHODS AND RESULTS:The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recomb...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Alberto F,Navarro D,de Vries RP,Asther M,Record E

    更新日期:2009-08-01 00:00:00

  • Cold storage affects survival and growth of Erwinia amylovora on the calyx of apple.

    abstract:AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Taylor RK,Hale CN

    更新日期:2003-01-01 00:00:00

  • Comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis in separating Escherichia coli uidA amplicons differing in single base substitutions.

    abstract::A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

    更新日期:2000-06-01 00:00:00

  • Pseudomonas syringae pv. japonica (Mukoo 1955) Dye et al. 1980 is a junior synonym of Ps. syringae pv. syringae van Hall 1902.

    abstract::An investigation of the biochemical, nutritional and pathogenic reactions of strains of Pseudomonas syringae pv. japonica and Ps. syringae pv. syringae showed them to be indistinguishable. Pseudomonas syringae pv. japonica is a junior synonym of Ps. syringae pv. syringae. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Young JM

    更新日期:1992-10-01 00:00:00

  • Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis.

    abstract:AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Berlec A,Tompa G,Slapar N,Fonović UP,Rogelj I,Strukelj B

    更新日期:2008-02-01 00:00:00

  • Comparison of disc diffusion, Etest and agar dilution for susceptibility testing of colistin against Enterobacteriaceae.

    abstract:AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Maalej SM,Meziou MR,Rhimi FM,Hammami A

    更新日期:2011-11-01 00:00:00

  • Adhesion capability of first two domains at N terminus of NP_785232 protein and their interaction with a UV-absorbing component from human mucus.

    abstract:AIMS:This work aims to investigate the binding capability of certain domains at N terminus of the protein NP_785232 of Lactobacillus plantarum to Caco-2 cells and to test the usage of affinity chromatography to isolate the human mucus component that interacts with them. METHODS AND RESULTS:Recombinant proteins contain...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Du L,Liu F,Ju X,Huo G

    更新日期:2010-10-01 00:00:00

  • Comparison of fermentative capacities of industrial baking and wild-type yeasts of the species Saccharomyces cerevisiae in different sugar media.

    abstract:AIMS:To compare the fermentative capacity of wild and domesticated isolates of the genus Saccharomyces. METHODS AND RESULTS:The fermentative capacity of yeasts from a variety of wild and domesticated sources was tested in synthetic dough media that mimic major bread dough types. Domesticated yeast strains were found t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Bell PJ,Higgins VJ,Attfield PV

    更新日期:2001-04-01 00:00:00

  • Alveolar macrophage reaction to Candida species.

    abstract::Candida species are increasingly important fungal pathogens. The reaction of rat alveolar macrophages (AM) to Candida albicans was compared with that to C. glabrata and C. krusei. Phagocytosis of C. glabrata was similar to that of C. albicans, but significantly slower for C. krusei due to reduced attachment. After ops...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Nessa K,Johansson A,Jarstrand C,Camner P

    更新日期:1997-09-01 00:00:00

  • Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys.

    abstract::The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Ferrandis MD,Andrew R,Porcar M,Iriarte J,Cosmao-Dumanoir V,Lecadet MM,Caballero P,Ferré J

    更新日期:1999-06-01 00:00:00

  • The fate of Salmonella enteritidis PT4 in home-made mayonnaise prepared with citric acid.

    abstract::The fate of Salmonella enteritidis PT4 in home-made mayonnaise prepared with citric acid solution (citric acid concentration of > or = 4.98% (w/v)) was investigated. It was found that pH of mayonnaise is closely related to the ratio of egg yolk to citric acid, and the inactivation rate of the micro-organisms increases...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Xiong R,Xie G,Edmondson AS

    更新日期:1999-01-01 00:00:00

  • Novel multiplex polymerase chain reaction primer set for identification of Lactococcus species.

    abstract:AIMS:The gram-positive bacterial genus Lactococcus has been taxonomically classified into seven species (Lactococcus lactis, Lactococcus garvieae, Lactococcus piscium, Lactococcus plantarum, Lactococcus raffinolactis, Lactococcus chungangensis and Lactococcus fujiensis). This study aimed to develop a novel multiplex po...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Odamaki T,Yonezawa S,Kitahara M,Sugahara Y,Xiao JZ,Yaeshima T,Iwatsuki K,Ohkuma M

    更新日期:2011-05-01 00:00:00

  • Anti-MRSA activity of sophoraflavanone G and synergism with other antibacterial agents.

    abstract::Anti-MRSA activity of sophoraflavanone G (SFG) and synergism between SFG and antibacterial agents against MRSA (methicillin-resistant Staphylococcus aureus) were evaluated by means of Minimal Inhibitory Concentrations (MIC). The MICs of SFG against 27 strains of MRSA ranged from 3.13 to 6.25 micrograms ml-1. Synergism...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Sakagami Y,Mimura M,Kajimura K,Yokoyama H,Linuma M,Tanaka T,Ohyama M

    更新日期:1998-08-01 00:00:00

  • The Bacillus secretion stress response is an indicator for alpha-amylase production levels.

    abstract:AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Westers H,Darmon E,Zanen G,Veening JW,Kuipers OP,Bron S,Quax WJ,van Dijl JM

    更新日期:2004-01-01 00:00:00

  • Evaluation of the specificity of Salmonella PCR primers using various intestinal bacterial species.

    abstract:AIMS:Nine sets of PCR primers targeting Salmonella were evaluated for their specificity with pure cultures of intestinal-associated bacteria prior to their application to Salmonella detection in faecal samples. METHODS AND RESULTS:Gene targets of PCR primers included: 16S rDNA, a Salmonella pathogenicity island I viru...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Ziemer CJ,Steadham SR

    更新日期:2003-01-01 00:00:00

  • Improved antimicrobial activity of nisin-incorporated polymer films by formulation change and addition of food grade chelator.

    abstract:AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Cutter CN,Willett JL,Siragusa GR

    更新日期:2001-10-01 00:00:00

  • Usage of a selective media (EMJH-STAFF) in primary culturing of pathogenic leptospires from bovine clinical samples.

    abstract:UNLABELLED:Isolation of local strains is mandatory for the success of control programs. However, clinical samples are typically contaminated by other bacteria, which impair leptospires growth. The purpose of this study was to evaluate the use of a previously reported EMJH-STAFF media in the recovery of pathogenic lepto...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Loureiro AP,Martins G,Pinto P,Narduche L,Teixeira RC,Lilenbaum W

    更新日期:2015-12-01 00:00:00

  • Development of an enzyme-linked immunosorbent assay for Bartonella henselae infection detection.

    abstract:UNLABELLED:Several serological diagnostics rely on enzyme-linked immunosorbent assay (ELISA) to detect bacterial infections. However, for some pathogens, including Bartonella henselae, diagnosis still depends on manually intensive, time-consuming assays including micro-immunofluorescence, Western blotting or indirect i...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Ferrara F,Di Niro R,D'Angelo S,Busetti M,Marzari R,Not T,Sblattero D

    更新日期:2014-09-01 00:00:00

  • Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood.

    abstract::The Andrias davidianus has been known as a traditional Chinese medicine for a long time. Its blood is considered as a waste or by-product of the meat production industry. Although there are reports on isolation of the antimicrobial peptides from different resources, there are no reports of their isolation from A. davi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Pei J,Feng Z,Ren T,Sun H,Han H,Jin W,Dang J,Tao Y

    更新日期:2018-01-01 00:00:00

  • The incidence and PCR detection of Campylobacter upsaliensis in dogs and cats.

    abstract::A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Steinhauserova I,Fojtikova K,Klimes J

    更新日期:2000-09-01 00:00:00

  • Development of an intergeneric conjugal transfer system for rimocidin-producing Streptomyces rimosus.

    abstract:AIMS:To develop an intergeneric conjugation system for rimocidin-producing Streptomyces rimosus. METHODS AND RESULTS:High efficiencies of conjugation [10(-2)-10(-3) transconjugants/recipient colony forming units (CFU)] were obtained when spores of S. rimosus were heat treated at 40 degrees C for 10 min prior to mixing...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Phornphisutthimas S,Sudtachat N,Bunyoo C,Chotewutmontri P,Panijpan B,Thamchaipenet A

    更新日期:2010-05-01 00:00:00

  • Cultivable bacterial diversity from the human colon.

    abstract::Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审


    authors: Duncan SH,Louis P,Flint HJ

    更新日期:2007-04-01 00:00:00

  • The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin.

    abstract::The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

    更新日期:2000-04-01 00:00:00

  • Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard-shelled mussel (Mytilus coruscus) in Korea.

    abstract::Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. cavi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Hossain S,Wickramanayake MVKS,Dahanayake PS,Heo GJ

    更新日期:2020-03-01 00:00:00

  • Assessment of the bacterial diversity of breast milk of healthy women by quantitative real-time PCR.

    abstract:AIMS:Breast milk has been described as a source of bacteria influencing the development of the infant gut microbiota. Up to the present, few studies have been focused on the application of culture-independent techniques to study bacterial diversity in breast milk. In this context, the aim of this study was to character...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Collado MC,Delgado S,Maldonado A,Rodríguez JM

    更新日期:2009-05-01 00:00:00