An in-vitro study on a novel six-phage cocktail against multi-drug resistant-ESBL Shigella in aquatic environment.

abstract：:The emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. As an example, Staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last ...

journal_title：Letters in applied microbiology

authors： Rihakova J,Belguesmia Y,Petit VW,Pilet MF,Prévost H,Dousset X,Drider D

更新日期：2009-01-01 00:00:00

abstract：:Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world. Coffee leaf blight, caused by Phomopsis heveicola, is one of the most important fungal diseases dangerous to coffee crops in China. This study aimed to develop a PCR-...

journal_title：Letters in applied microbiology

authors： Lu Y,Wu WH,He CP,Liang YQ,Huang X,Zheng JL,Xi JG,Tang SB,Yi KX

更新日期：2020-09-26 00:00:00

abstract：AIMS:Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS. METHODS AND RESULTS:Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, f...

journal_title：Letters in applied microbiology

authors： Tinoco R,Pickard MA,Vazquez-Duhalt R

更新日期：2001-05-01 00:00:00

abstract：AIMS:To investigate the growth of salmonellae on sprouting alfalfa seeds as affected by the inoculum size, microbial load and Pseudomonas fluorescens 2-79. METHODS AND RESULTS:Alfalfa seeds pre-inoculated with < or =10(1)-10(3) CFU g(-1) of salmonellae and with or without Ps. fluorescens 2-79 were sprouted in glass ja...

journal_title：Letters in applied microbiology

authors： Liao CH

更新日期：2008-02-01 00:00:00

abstract：UNLABELLED:Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysa...

journal_title：Letters in applied microbiology

authors： Song A,You Y,Chen F,Li P,Jiang J,Chen S

更新日期：2013-01-01 00:00:00

abstract：:An investigation of the biochemical, nutritional and pathogenic reactions of strains of Pseudomonas syringae pv. japonica and Ps. syringae pv. syringae showed them to be indistinguishable. Pseudomonas syringae pv. japonica is a junior synonym of Ps. syringae pv. syringae. ...

journal_title：Letters in applied microbiology

authors： Young JM

更新日期：1992-10-01 00:00:00

abstract：AIMS:To evaluate the specificity and sensitivity of PCR primers for the detection of Salmonella enterica in a real-time PCR assay using pure cultures. METHODS AND RESULTS:Unenriched whole cells in sterile water were used as template for each PCR. SYBR Green dye was used for the nonspecific detection of dsDNA. The real...

journal_title：Letters in applied microbiology

authors： Csordas AT,Barak JD,Delwiche MJ

更新日期：2004-01-01 00:00:00

abstract：:The beta-D-galactosidase activity of viable but non-culturable (vnc) Escherichia coli cells in seawater was investigated using a rapid fluorimetric enzyme assay. Results from microcosm studies showed that loss of culturability did not necessarily result in loss of the ability to produce the galactosidase enzyme. Even ...

journal_title：Letters in applied microbiology

authors： Davies CM,Apte SC,Peterson SM

更新日期：1995-08-01 00:00:00

abstract：:Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...

journal_title：Letters in applied microbiology

authors： Yuste J,Pla R,Mor-Mur M

更新日期：2000-11-01 00:00:00

abstract：AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...

journal_title：Letters in applied microbiology

authors： Sudhakaran R,Okugawa S,Mekata T,Inada M,Yoshimine M,Nishi J,Ozono C,Kono T,Sakai M,Itami T

更新日期：2011-07-01 00:00:00

abstract：:Chronic Pseudomonas aeruginosa infection is associated with increased morbidity and mortality in patients with cystic fibrosis (CF). Current understanding of risk factors for acquisition is limited and so the aim of this study was to examine a large sample of environmental waters from diverse sources. Environmental wa...

journal_title：Letters in applied microbiology

authors： Caskey S,Stirling J,Moore JE,Rendall JC

更新日期：2018-06-01 00:00:00

abstract：AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

journal_title：Letters in applied microbiology

authors： Park JP,Yun JW

更新日期：2001-09-01 00:00:00

abstract：AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...

journal_title：Letters in applied microbiology

authors： Chen JC,Lin ZH,Ma XX

更新日期：2003-01-01 00:00:00

abstract：AIMS:To evaluate the genetic diversity within the species Paenibacillus polymyxa. METHODS:Southern hybridization was performed on 102 strains of P. polymyxa using DNA from the phage IPy1 as a probe. RESULTS:All 102 strains hybridized to phage IPy1 DNA. Data from different hybridization patterns obtained were used to ...

journal_title：Letters in applied microbiology

authors： Santos SC,Rodrigues Coelho MR,Seldin L

更新日期：2002-01-01 00:00:00

abstract：:Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n = 63), chicken (n = 50) and turkey (n = 11) collected from retail outlets in North West England between March and July 2015. MR...

journal_title：Letters in applied microbiology

authors： Fox A,Pichon B,Wilkinson H,Doumith M,Hill RL,McLauchlin J,Kearns AM

更新日期：2017-03-01 00:00:00

abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：AIMS:To demonstrate that produce rinsates used for RT-qPCR detection of foodborne viruses may cause significant PCR inhibition and propose a means to reduce its impact on sensitivity. METHODS AND RESULTS:Here, it is shown that rinsing and concentration from spinach and precut lettuce have the potential to generate RNA...

journal_title：Letters in applied microbiology

authors： Plante D,Bélanger G,Leblanc D,Ward P,Houde A,Trottier YL

更新日期：2011-03-01 00:00:00

abstract：AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...

journal_title：Letters in applied microbiology

authors： Myers JM,Myers CR

更新日期：2003-01-01 00:00:00

abstract：:This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...

journal_title：Letters in applied microbiology

authors： Willinger B,Manafi M

更新日期：1995-05-01 00:00:00

abstract：:The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applic...

journal_title：Letters in applied microbiology

authors： Ma Y,Dai T,Serwadda A,Shen H

更新日期：2016-11-01 00:00:00

abstract：:The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...

journal_title：Letters in applied microbiology

authors： Coutinho HL,Handley BA,Kay HE,Stevenson L,Beringer JE

更新日期：1993-12-01 00:00:00

abstract：AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...

journal_title：Letters in applied microbiology

authors： Vettraino AM,Bonants P,Tomassini A,Bruni N,Vannini A

更新日期：2012-11-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

journal_title：Letters in applied microbiology

authors： McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

更新日期：2005-01-01 00:00:00

abstract：UNLABELLED:Most plant species are colonized by endophytic bacteria. Despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. Hence, we investigated the bacterial community structure in three agricultural important grass species Dactyli...

journal_title：Letters in applied microbiology

authors： Wemheuer F,Wemheuer B,Kretzschmar D,Pfeiffer B,Herzog S,Daniel R,Vidal S

更新日期：2016-04-01 00:00:00

abstract：:Seventeen aerobic bacterial strains known to degrade one or more aromatic compounds were tested for their ability to utilize benzene, seven different methylated benzenes or other aromatic compounds as their sole source of carbon. The results are discussed with respect to the possibility of finding strains which degrad...

journal_title：Letters in applied microbiology

authors： Lang E

更新日期：1996-10-01 00:00:00

abstract：AIM:Combination of immunomagnetic separation (IMS) and lateral flow device (LFD) assays for the development of a sensitive, rapid, on-site methodology that enables concentration and detection of Bacillus anthracis spores in complex samples. METHODS AND RESULTS:The data presents the development of an optimized, 30 min,...

journal_title：Letters in applied microbiology

authors： Fisher M,Atiya-Nasagi Y,Simon I,Gordin M,Mechaly A,Yitzhaki S

更新日期：2009-04-01 00:00:00

abstract：UNLABELLED:Faecal indicator bacteria (FIB) and harmful algal blooms (HABs) threaten the health and the economy of coastal communities worldwide. Emerging automated sampling technologies combined with molecular analytical techniques could enable rapid detection of micro-organisms in-situ, thereby improving resource mana...

journal_title：Letters in applied microbiology

authors： Yamahara KM,Demir-Hilton E,Preston CM,Marin R 3rd,Pargett D,Roman B,Jensen S,Birch JM,Boehm AB,Scholin CA

更新日期：2015-08-01 00:00:00

abstract：AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

journal_title：Letters in applied microbiology

authors： Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

更新日期：2007-09-01 00:00:00

abstract：:A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

journal_title：Letters in applied microbiology

authors： Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

更新日期：1998-05-01 00:00:00