The effect of colony age on PCR fingerprinting.

abstract：AIMS:To identify several strains of Mesorhizobium amorphae and Mesorhizobium tianshanense nodulating Cicer arietinum in Spain and Portugal, and to study the symbiotic genes carried by these strains. METHODS AND RESULTS:The sequences of 16S-23S intergenic spacer (ITS), 16S rRNA gene and symbiotic genes nodC and nifH we...

journal_title：Letters in applied microbiology

authors： Rivas R,Laranjo M,Mateos PF,Oliveira S,Martínez-Molina E,Velázquez E

更新日期：2007-04-01 00:00:00

abstract：UNLABELLED:Industrial biotechnology uses microbiological cells to produce a wide range of products. While the organisms in question are well understood regarding their genetic and molecular properties, less is known about their mechanical properties. Previous work has established a testing procedure for single Saccharo...

journal_title：Letters in applied microbiology

authors： Overbeck A,Kampen I,Kwade A

更新日期：2015-10-01 00:00:00

abstract：:A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic Europ...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Rasmussen OF

更新日期：1994-11-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：AIMS:Tuberculous pleurisy is an important cause of pleural effusions in areas with a high incidence of tuberculosis. In this study, we developed an IS1081-based LAMP for the detection of Mycobacterium tuberculosis complex and investigated its usefulness in the diagnosis of tuberculous pleurisy. METHODS AND RESULTS:Inv...

journal_title：Letters in applied microbiology

authors： Yang B,Wang X,Li H,Li G,Cao Z,Cheng X

更新日期：2011-11-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

journal_title：Letters in applied microbiology

authors： Rogers JV,Ducatte GR,Choi YW,Early PC

更新日期：2006-11-01 00:00:00

abstract：AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...

journal_title：Letters in applied microbiology

authors： Gilbert SE,Rose LJ

更新日期：2012-09-01 00:00:00

abstract：:The emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. As an example, Staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last ...

journal_title：Letters in applied microbiology

authors： Rihakova J,Belguesmia Y,Petit VW,Pilet MF,Prévost H,Dousset X,Drider D

更新日期：2009-01-01 00:00:00

abstract：AIMS:A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS:Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was add...

journal_title：Letters in applied microbiology

authors： Kang DH,Siragusa GR

更新日期：2001-09-01 00:00:00

abstract：:A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml-1, as found in ...

journal_title：Letters in applied microbiology

authors： Brooks T,Keevil CW

更新日期：1997-03-01 00:00:00

abstract：:Understanding mechanisms of the novel SARS-CoV2 infection and progression can provide potential novel targets for prevention and/or treatment. This could be achieved via the inhibition of viral entry and/or replication, or by suppression of the immunologic response that is provoked by the infection (known as the cytok...

journal_title：Letters in applied microbiology

authors： Akour A

更新日期：2020-09-01 00:00:00

abstract：:Novel findings in microbiology question the long-standing paradigm that a healthy pregnancy implies a sterile uterus. It now seems that the placenta is frequently colonized with bacteria, and a placental microbiome has been identified. Recent literature findings are summarized here, and an attempt is made to separate ...

journal_title：Letters in applied microbiology

authors： Wassenaar TM,Panigrahi P

更新日期：2014-12-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：AIM:To identify the gene that encodes nigrescin, a bacteriocin produced by Prevotella nigrescens ATCC 25261. METHODS AND RESULTS:Each open reading frame (ORF) of the nig gene cluster (nigA, nigB, nigC and nigD) was transferred into an expression vector. The recombinant proteins encoded by nigA, nigB, nigC and nigD wer...

journal_title：Letters in applied microbiology

authors： Teanpaisan R,Narawatthana S,Utarabhand P

更新日期：2009-09-01 00:00:00

abstract：AIMS:To develop a specific, fast and simple molecular method useful to detect the entomopathogenic bacterium Pseudomonas entomophila. METHODS AND RESULTS:The use of bioinformatics tools allowed the identification of unique genes present in P. entomophila genome. Using such genes, we designed primers aiming to detect s...

journal_title：Letters in applied microbiology

authors： Papagiannoulis A,Mathiopoulos KD,Mossialos D

更新日期：2010-03-01 00:00:00

abstract：:The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, ...

journal_title：Letters in applied microbiology

authors： Yang N,Wang X,Teng D,Mao R,Hao Y,Feng X,Wang J

更新日期：2017-09-01 00:00:00

abstract：AIMS:In this study, the relationship between morphology and cephalosporin C (CPC) production in a 30-l bioreactor culture of Cephalosporium acremonium M25 using a 3:7 seed mixture was investigated. In addition, the kinetic model was established and applied. METHODS AND RESULTS:CPC production was performed in a 30-l bi...

journal_title：Letters in applied microbiology

authors： Kim JH,Lim JS,Kim CH,Kim SW

更新日期：2005-01-01 00:00:00

abstract：:Two commercially available screening methods, an automated enzyme-linked fluorescent immunoassay (VIDAS E. coli O157) and an immunomagnetic separation followed by culture onto cefixime tellurite sorbitol MacConkey agar (CT-SMAC), were compared for detection of Escherichia coli O157 in naturally and artificially contam...

journal_title：Letters in applied microbiology

authors： Vernozy-Rozand C,Mazuy C,Ray-Gueniot S,Boutrand-Loeï S,Meyrand A,Richard Y

更新日期：1997-12-01 00:00:00

abstract：:Environmental air sampling was evaluated as a method to detect the presence of M. bovis in the vicinity of infected badgers and their setts. Airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of M. bovis using bacteriological culture...

journal_title：Letters in applied microbiology

authors： Jones RM,Ashford R,Cork J,Palmer S,Wood E,Spyvee P,Parks S,Bennett A,Brewer J,Delahay R,Chambers M,Sawyer J

更新日期：2013-05-01 00:00:00

abstract：:This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n = 92) and backyard (n = 110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87...

journal_title：Letters in applied microbiology

authors： Banerjee A,Bardhan R,Chowdhury M,Joardar SN,Isore DP,Batabyal K,Dey S,Sar TK,Bandyopadhyay S,Dutta TK,Samanta I

更新日期：2019-08-01 00:00:00

abstract：:Emulsions of the fatty acids linoleic (C18:2 n-6), alpha-linolenic (C18:3 n-3) and arachidonic acid (C20:4 n-6) were incubated for 4 h under anaerobic conditions with human faecal suspensions. Linoleic acid was significantly decreased (P < 0.001) and there was a significant rise (P < 0.05) in its hydrogenation product...

journal_title：Letters in applied microbiology

authors： Howard FA,Henderson C

更新日期：1999-09-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：AIMS:To screen for the existence and determine the structure of Tn916-like element in Streptococcus parauberis serotype II strains isolated from cultured Japanese flounder in western Japan. METHODS AND RESULTS:In this study, the structure of Tn916-like element and the flanking regions were characterized by polymerase ...

journal_title：Letters in applied microbiology

authors： Meng F,Kanai K,Yoshikoshi K

更新日期：2009-06-01 00:00:00

abstract：AIMS:To examine changes in membrane fatty acid profile attributed to the physiological adaptation of Lactobacillus helveticus during vacuum drying. METHODS AND RESULTS:The viability and membrane integrity of the cells after vacuum drying were measured by plate counts and DNA fluorescence dyes. The physiological adapta...

journal_title：Letters in applied microbiology

authors： Santivarangkna C,Kulozik U,Kienberger H,Foerst P

更新日期：2009-10-01 00:00:00

abstract：UNLABELLED:During a screening for potential plant disease control actinomycetes, a total of 133 strains were isolated from Saharan soil samples of seven Algerian regions by dilution technique on chitin-vitamins agar medium. Screening for antagonistic properties using streak assay method showed that 25% of isolates demo...

journal_title：Letters in applied microbiology

authors： Yekkour A,Sabaou N,Zitouni A,Errakhi R,Mathieu F,Lebrihi A

更新日期：2012-12-01 00:00:00

abstract：AIMS:This work aims to investigate the binding capability of certain domains at N terminus of the protein NP_785232 of Lactobacillus plantarum to Caco-2 cells and to test the usage of affinity chromatography to isolate the human mucus component that interacts with them. METHODS AND RESULTS:Recombinant proteins contain...

journal_title：Letters in applied microbiology

authors： Du L,Liu F,Ju X,Huo G

更新日期：2010-10-01 00:00:00

abstract：AIMS:Natural and synthetic antimicrobial peptides (AMPs) are of increasing interest as potential resistance conferring elements in plants against pathogen infection. The efficacy of AMPs against pathogens is prescreened by in vitro assays, and promising AMP candidates are introduced as transgenes into plants. As in vit...

journal_title：Letters in applied microbiology

authors： Visser M,Stephan D,Jaynes JM,Burger JT

更新日期：2012-06-01 00:00:00

abstract：:The L-2,3-butanediol dehydrogenase (L-BDH) gene of Brevibacterium saccharolyticum was strongly expressed in Escherichia coli using the tac promoter. However, the stereospecificity of the resulting L-BDH was reduced. The region upstream of the meso-BDH gene of Klebsiella pneumoniae was also involved in the expression o...

journal_title：Letters in applied microbiology

authors： Ui S,Takusagawa Y,Ohtsuki T,Mimura A,Ohkuma M,Kudo T

更新日期：2001-02-01 00:00:00

abstract：AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (desig...

journal_title：Letters in applied microbiology

authors： Deshpande NM,Dhakephalkar PK,Kanekar PP

更新日期：2001-10-01 00:00:00