Development and evaluation of loop-mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens.

Abstract:

AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designing two sets of outer and inner primers, which target a total of six distinct regions on VP2 gene of CPV. The template DNA was prepared by a simple boiling and chilling method. Of the 140 faecal samples screened by the developed LAMP and the conventional PCR assays, 104 samples (74·28%) were found positive by LAMP, whereas 81 samples (57·85%) were found positive by PCR. The specificity of the LAMP assay was tested by cross-examination of common pathogens of dogs and further confirmed by sequencing. The detection limit of the LAMP was 0·0001 TCID₅₀ ml⁻¹, whereas the detection limit of the PCR was 1000 TCID₅₀ ml⁻¹. CONCLUSIONS: The developed LAMP assay detects CPV DNA in faecal specimens directly within an hour by following a simple and rapid boiling and chilling method of template preparation. The result also shows that the developed LAMP assay is specific and highly sensitive in detecting CPV. SIGNIFICANCE AND IMPACT OF THE STUDY: The result indicates the potential usefulness of LAMP which is a simple, rapid, specific, highly sensitive and cost-effective field-based method for direct detection of CPV from the suspected faecal samples of dogs.

journal_name

Lett Appl Microbiol

authors

Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

doi

10.1111/j.1472-765X.2012.03284.x

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

202-9

issue

3

eissn

0266-8254

issn

1472-765X

journal_volume

55

pub_type

杂志文章
  • A combined immunomagnetic separation and lateral flow method for a sensitive on-site detection of Bacillus anthracis spores--assessment in water and dairy products.

    abstract:AIM:Combination of immunomagnetic separation (IMS) and lateral flow device (LFD) assays for the development of a sensitive, rapid, on-site methodology that enables concentration and detection of Bacillus anthracis spores in complex samples. METHODS AND RESULTS:The data presents the development of an optimized, 30 min,...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02542.x

    authors: Fisher M,Atiya-Nasagi Y,Simon I,Gordin M,Mechaly A,Yitzhaki S

    更新日期:2009-04-01 00:00:00

  • Flurbiprofen, a unique non-steroidal anti-inflammatory drug with antimicrobial activity against Trichophyton, Microsporum and Epidermophyton species.

    abstract:AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01370.x

    authors: Chowdhury B,Adak M,Bose SK

    更新日期:2003-01-01 00:00:00

  • Cellulose acetate-based composites with antimicrobial properties from embedded molybdenum trioxide particles.

    abstract::The objective of this research was to develop novel cellulose acetate (biopolymer) composite materials with an excellent antimicrobial activity by embedding molybdenum trioxide particles with unique high specific surface area. High surface area molybdenum trioxide particles were prepared from freshly precipitated moly...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12670

    authors: Shafaei S,Dörrstein J,Guggenbichler JP,Zollfrank C

    更新日期:2017-01-01 00:00:00

  • Biosurfactant production by Pseudomonas sp. and its role in aqueous phase partitioning and biodegradation of chlorpyrifos.

    abstract:AIM:To study the effect of biosurfactant on aqueous phase solubility and biodegradation of chlorpyrifos. METHODS AND RESULTS:A Pseudomonas sp. (ChlD), isolated from agricultural soil by enrichment culture technique in the presence of chlorpyrifos, was capable of producing biosurfactant (rhamnolipids) and degrading chl...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02672.x

    authors: Singh PB,Sharma S,Saini HS,Chadha BS

    更新日期:2009-09-01 00:00:00

  • The prevalence of opportunistic pathogens associated with intraoral implants.

    abstract:AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03031.x

    authors: Al Groosh D,Roudsari GB,Moles DR,Ready D,Noar JH,Pratten J

    更新日期:2011-05-01 00:00:00

  • Shiga toxigenic and enteropathogenic Escherichia coli in water and fish from pay-to-fish ponds.

    abstract:UNLABELLED:Escherichia coli is part of the normal microflora of the intestines of mammals. However, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially Shiga toxigenic E. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12536

    authors: Ribeiro LF,Barbosa MM,de Rezende Pinto F,Guariz CS,Maluta RP,Rossi JR,Rossi GA,Lemos MV,do Amaral LA

    更新日期:2016-03-01 00:00:00

  • Iron uptake is essential for Escherichia coli survival in drinking water.

    abstract:AIMS:The aim of this study was to elucidate if the need for iron for Escherichia coli to remain cultivable in a poorly nutritive medium such as the drinking water uses the iron transport system via the siderophores. METHODS AND RESULTS:Environmental strains of E. coli (isolated from a drinking water network), referenc...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01895.x

    authors: Grandjean D,Jorand F,Guilloteau H,Block JC

    更新日期:2006-07-01 00:00:00

  • Lactobacilli prevent hydroxy radical production and inhibit Escherichia coli and Enterococcus growth in system mimicking colon fermentation.

    abstract:AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02786.x

    authors: Sun J,Hu XL,Le GW,Shi YH

    更新日期:2010-03-01 00:00:00

  • Discrepancies between the phenotypic and genotypic characterization of Lactococcus lactis cheese isolates.

    abstract:AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02012.x

    authors: de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

    更新日期:2006-12-01 00:00:00

  • Comparison of three DNA extraction methods for Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium.

    abstract:AIMS:To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. METHODS AND RESULTS:The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02372.x

    authors: Amaro A,Duarte E,Amado A,Ferronha H,Botelho A

    更新日期:2008-07-01 00:00:00

  • Reduction of Saccharomyces cell adhesion by liquid mechanical vibration.

    abstract::The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb00022.x

    authors: Jiang C,Yu H,Ding Z,Ma Y

    更新日期:1996-07-01 00:00:00

  • Occurrence of Pseudomonas aeruginosa in waters: implications for patients with cystic fibrosis (CF).

    abstract::Chronic Pseudomonas aeruginosa infection is associated with increased morbidity and mortality in patients with cystic fibrosis (CF). Current understanding of risk factors for acquisition is limited and so the aim of this study was to examine a large sample of environmental waters from diverse sources. Environmental wa...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12876

    authors: Caskey S,Stirling J,Moore JE,Rendall JC

    更新日期:2018-06-01 00:00:00

  • Influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in a defined medium.

    abstract:AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01237.x

    authors: Dykes GA,Dworaczek M

    更新日期:2002-01-01 00:00:00

  • A rapid and sensitive method for screening of chitinase inhibitors using Ostazin Brilliant Red labelled chitin as a substrate for chitinase assay.

    abstract:AIMS:The development of a simple, rapid and sensitive method for the screening of chitinase inhibitors using Ostazin Brilliant Red labelled chitin as a substrate for chitinase assay. METHODS AND RESULTS:The colloidal substrate prepared from the Ostazin Brilliant Red labelled chitin was used in the estimation of chitin...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02117.x

    authors: Shirazi F,Kulkarni M,Deshpande MV

    更新日期:2007-06-01 00:00:00

  • Bacillus cereus and Bacillus thuringiensis isolated in a gastroenteritis outbreak investigation.

    abstract::During investigation of a gastroenteritis outbreak in a chronic care institution, Norwalk virus was found in stool specimens from two individuals and bacterial isolates presumptively identified as Bacillus cereus were isolated from four individuals (including one with Norwalk virus) and spice. Phage typing confirmed a...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb01017.x

    authors: Jackson SG,Goodbrand RB,Ahmed R,Kasatiya S

    更新日期:1995-08-01 00:00:00

  • Development of a simple and rapid Agrobacterium tumefaciens-mediated transformation system for the entomopathogenic fungus Metarhizium anisopliae var. acridum.

    abstract:AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02092.x

    authors: Duarte RT,Staats CC,Fungaro MH,Schrank A,Vainsten MH,Furlaneto-Maia L,Nakamura CV,de Souza W,Furlaneto MC

    更新日期:2007-03-01 00:00:00

  • Occurrence of fungi in water used at a haemodialysis centre.

    abstract:AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02349.x

    authors: Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

    更新日期:2008-05-01 00:00:00

  • Phenotypic and genotypic diversity of Genista saharae microsymbionts from the infra-arid region of Tunisia.

    abstract:AIMS:Genista saharae, indigenous of Sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. This legume has not been examined for its root nodule bacteria. The taxonomic diversity of bacteria from root nodules of G. saharae growing in the infra...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02233.x

    authors: Mahdhi M,Nzoué A,Gueye F,Merabet C,de Lajudie P,Mars M

    更新日期:2007-12-01 00:00:00

  • New erythromycin derivatives enhance β-lactam antibiotics against methicillin-resistant Staphylococcus aureus.

    abstract:UNLABELLED:Newly synthesized erythromycin derivatives were screened for synergy with oxacillin and other β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). MRSA ATCC43300 and some clinically isolated MRSA were used. Several erythromycin derivatives were found to possess high synergism with...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12378

    authors: Li Z,He M,Dong X,Lin H,Ge H,Shen S,Li J,Ye RD,Chen D

    更新日期:2015-04-01 00:00:00

  • Molasses supplementation promotes conidiation but suppresses aflatoxin production by small sclerotial Aspergillus flavus.

    abstract:AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02056.x

    authors: Chang PK,Hua SS

    更新日期:2007-02-01 00:00:00

  • Efficacy of different caffeine concentrations on growth and ochratoxin A production by Aspergillus species.

    abstract:UNLABELLED:The objective of this study was to evaluate the effect of different caffeine concentrations (0-4%) on (i) lag phase prior to growth, (ii) growth rates and (iii) ochratoxin A (OTA) production by strains from the Aspergillus section Circumdati and Aspergillus section Nigri groups, isolated from coffee, when gr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12586

    authors: Akbar A,Medina A,Magan N

    更新日期:2016-07-01 00:00:00

  • Analysis of expressed sequence tags from Chaetomium cupreum grown under conditions associated with mycoparasitism.

    abstract:AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02487.x

    authors: Zhang HY,Yang Q,Wang G,Shang FD

    更新日期:2009-03-01 00:00:00

  • Anomalies in species identification of enterococci from veterinary sources using a commercial biochemical identification system.

    abstract:AIMS:A commercial biochemical panel ID kit was used to identify presumptive enterococci isolates of veterinary or agricultural origin obtained during different steps of culture. METHODS AND RESULTS:Fifty isolates identified as enterococci using a genus PCR assay were tested for genus and species identification using t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01302.x

    authors: Hudson CR,Fedorka-Cray PJ,Jackson-Hall MC,Hiott LM

    更新日期:2003-01-01 00:00:00

  • Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549(T).

    abstract:AIMS:The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549(T). METHODS AND RESULTS:Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01189.x

    authors: Alexeeva YV,Ivanova EP,Bakunina IY,Zvaygintseva TN,Mikhailov VV

    更新日期:2002-01-01 00:00:00

  • Heat shock response enhances acid tolerance of Escherichia coli O157:H7.

    abstract::Escherichia coli O157:H7 (O157) has unusual acid tolerance. The influence of heat shock on acid tolerance of O157 was studied. Seven strains of O157 and E. coli K-12 were tested for their ability to survive in minimum glucose medium (pH 2.5) at 37 degrees C. The survival of heat-shocked (10 min at 48 degrees C) cells ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00264.x

    authors: Wang G,Doyle MP

    更新日期:1998-01-01 00:00:00

  • Antimicrobial efficacy of cryotreatment against Enterococcus faecalis in root canals.

    abstract::The purpose of this investigation was to evaluate intracanal bacterial reduction by cryotreatment using a dental instrument equipped with a duct and connected to a cryogenic fluid source. A total of 86 roots were infected with Enterococcus faecalis and incubated. After incubation, the contaminated roots were divided i...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12017

    authors: Mandras N,Allizond V,Bianco A,Banche G,Roana J,Piazza L,Viale P,Cuffini AM

    更新日期:2013-02-01 00:00:00

  • A transient expression assay for the in planta efficacy screening of an antimicrobial peptide against grapevine bacterial pathogens.

    abstract:AIMS:Natural and synthetic antimicrobial peptides (AMPs) are of increasing interest as potential resistance conferring elements in plants against pathogen infection. The efficacy of AMPs against pathogens is prescreened by in vitro assays, and promising AMP candidates are introduced as transgenes into plants. As in vit...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03244.x

    authors: Visser M,Stephan D,Jaynes JM,Burger JT

    更新日期:2012-06-01 00:00:00

  • Comparison of bioMérieux API 20NE and Remel RapID NF Plus, identification systems of type strains of Ralstonia pickettii.

    abstract:UNLABELLED:A : Comparison of two commercial miniaturized rapid systems for the identification of Ralstonia pickettii strains. METHODS AND RESULTS:Varying identification results were encountered using the bioMérieux API NE system and the Remel IDS RapID NF Plus commercial systems for R. pickettii. To compare these two ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01737.x

    authors: Adley CC,Saieb FM

    更新日期:2005-01-01 00:00:00

  • Identification of a ubiG-like gene involved in ubiquinone biosynthesis from Chlamydophila pneumoniae AR39.

    abstract:AIMS:To investigate if one hypothetical protein from Chlamydophila pneumoniae AR39 exerts UbiG-like function by complementary experiments. METHODS AND RESULTS:Proteins UbiG have a signature S-adenosylmethionine-binding motif compared with other methyltransferases. Probing with the conserved motif, one hypothetical pro...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02144.x

    authors: Liu J,Zhang X,Liu J

    更新日期:2007-07-01 00:00:00

  • Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens.

    abstract::Many strains of Pseudomonas fluorescens show potential for biological control of phytopathogens especially root pathogens. In taxonomic terms, several of them are indeed P. fluorescens sensu stricto, while others belong in fact to neighbouring species of the 'P. fluorescens' complex or to ill-defined related species w...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02566.x

    authors: Couillerot O,Prigent-Combaret C,Caballero-Mellado J,Moënne-Loccoz Y

    更新日期:2009-05-01 00:00:00