RAPD analysis of Yersinia enterocolitica.

abstract：:Plant proteases play a fundamental role in several processes like growth, development and in response to biotic and abiotic stress. In particular, aspartic proteases (AP) are expressed in different plant organs and have antimicrobial activity. Previously, we purified an AP from Salpichroa origanifolia fruits called sa...

journal_title：Letters in applied microbiology

authors： Díaz ME,Rocha GF,Kise F,Rosso AM,Guevara MG,Parisi MG

更新日期：2018-08-01 00:00:00

abstract：AIMS:The aim of the study was to investigate the antagonistic interactions of Trichoderma species against Rhizoctonia solani sclerotia by enhanced green fluorescence protein (EGFP)-tagged transformant of Trichoderma virens TY009. METHODS AND RESULTS:An EGFP was used as a report gene for transforming T. virens strain, ...

journal_title：Letters in applied microbiology

authors： Liu LN,Zhang JZ,Xu T

更新日期：2009-12-01 00:00:00

abstract：AIMS:Tuberculous pleurisy is an important cause of pleural effusions in areas with a high incidence of tuberculosis. In this study, we developed an IS1081-based LAMP for the detection of Mycobacterium tuberculosis complex and investigated its usefulness in the diagnosis of tuberculous pleurisy. METHODS AND RESULTS:Inv...

journal_title：Letters in applied microbiology

authors： Yang B,Wang X,Li H,Li G,Cao Z,Cheng X

更新日期：2011-11-01 00:00:00

abstract：:The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...

journal_title：Letters in applied microbiology

authors： Matteuzzi D,Brigidi P,Rossi M,Di D

更新日期：1990-10-01 00:00:00

abstract：AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...

journal_title：Letters in applied microbiology

authors： Khongkhaem P,Intasiri A,Luepromchai E

更新日期：2011-05-01 00:00:00

abstract：AIM:To examine whether isolates of the entomopathogenic fungus Beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. METHODS AND RESULTS:Primers for the traditional ITS1-5.8S-ITS2 region and two mi...

journal_title：Letters in applied microbiology

authors： Kouvelis VN,Ghikas DV,Edgington S,Typas MA,Moore D

更新日期：2008-03-01 00:00:00

abstract：:Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Soil physicochemical properties ...

journal_title：Letters in applied microbiology

authors： He Z,Chen H,Liang L,Dong J,Liang Z,Zhao L

更新日期：2019-06-01 00:00:00

abstract：AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...

journal_title：Letters in applied microbiology

authors： Cho YJ,Park JP,Hwang HJ,Kim SW,Choi JW,Yun JW

更新日期：2002-01-01 00:00:00

abstract：:The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

journal_title：Letters in applied microbiology

authors： Micklewright IJ,Sartory DP

更新日期：1995-09-01 00:00:00

abstract：AIMS:The gram-positive bacterial genus Lactococcus has been taxonomically classified into seven species (Lactococcus lactis, Lactococcus garvieae, Lactococcus piscium, Lactococcus plantarum, Lactococcus raffinolactis, Lactococcus chungangensis and Lactococcus fujiensis). This study aimed to develop a novel multiplex po...

journal_title：Letters in applied microbiology

authors： Odamaki T,Yonezawa S,Kitahara M,Sugahara Y,Xiao JZ,Yaeshima T,Iwatsuki K,Ohkuma M

更新日期：2011-05-01 00:00:00

abstract：AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

journal_title：Letters in applied microbiology

authors： Park JP,Yun JW

更新日期：2001-09-01 00:00:00

abstract：AIMS:The aims of this study are to obtain the draft genome sequence of Streptomyces coelicoflavus ZG0656, which produces novel acarviostatin family α-amylase inhibitors, and then to reveal the putative acarviostatin-related gene cluster and the biosynthetic pathway. METHODS AND RESULTS:The draft genome sequence of S. ...

journal_title：Letters in applied microbiology

authors： Guo X,Geng P,Bai F,Bai G,Sun T,Li X,Shi L,Zhong Q

更新日期：2012-08-01 00:00:00

abstract：:Typical haemolytic uraemic syndrome (STEC-HUS), caused by Shiga toxin (Stx)-producing Escherichia coli (STEC), is a serious, life-threating disease that mainly affects children. Bacteriological and genetic tests are commonly used in the routine laboratory diagnosis of STEC-HUS; however, serological methods have emerge...

journal_title：Letters in applied microbiology

authors： Rastawicki W,Śmietańska K,Rokosz-Chudziak N,Wołkowicz T

更新日期：2020-06-01 00:00:00

abstract：:Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...

journal_title：Letters in applied microbiology

authors： Erdem B,Threlfall EJ,Schofield SL,Ward LR,Rowe B

更新日期：1994-10-01 00:00:00

abstract：:Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

journal_title：Letters in applied microbiology

authors： Makkar HP,Becker K

更新日期：1997-10-01 00:00:00

abstract：AIMS:To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. METHODS AND RESULTS:Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory...

journal_title：Letters in applied microbiology

authors： Henriques IS,Fonseca F,Alves A,Saavedra MJ,Correia A

更新日期：2008-12-01 00:00:00

abstract：:Samples were collected from 148 adult cats, processed for isolation of Staphylococcus species and tested for susceptibility to penicillin G, gentamicin, oxacillin, amoxycillin, ampicillin, cephalexin and rifampin. Methicillin resistance was also determined. Ninety-eight isolates were obtained (66% of samples). Coagula...

journal_title：Letters in applied microbiology

authors： Lilenbaum W,Nunes EL,Azeredo MA

更新日期：1998-10-01 00:00:00

abstract：AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

journal_title：Letters in applied microbiology

authors： Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

更新日期：2008-05-01 00:00:00

abstract：:A 21-mer DNA oligonucleotide probe targeting the 23S rRNA of Mycobacterium leprae was developed and its high specificity demonstrated by dot-blot hybridization. Even under relaxed hybridization and washing conditions (20 degrees C below Tm) the probe was highly selective in that positive signals were only detected wit...

journal_title：Letters in applied microbiology

authors： Liesack W,Pitulle C,Stackebrandt E

更新日期：1990-08-01 00:00:00

abstract：AIMS:The current standard culture methods are unable to detect nongrowing bacteria and, thus, might not be sufficient for precise monitoring of the microbiological quality of waters. The use of a molecular method such as PCR could be a valid alternative to detect bacterial faecal contamination indicators such as Escher...

journal_title：Letters in applied microbiology

authors： Lleo MM,Bonato B,Tafi MC,Signoretto C,Pruzzo C,Canepari P

更新日期：2005-01-01 00:00:00

abstract：:Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the ...

journal_title：Letters in applied microbiology

authors： Weijtens MJ,Urlings HA,Van der Plas J

更新日期：2000-06-01 00:00:00

abstract：AIMS:The present study was aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Vibrio cholerae. METHODS AND RESULTS:A set of five designed primers that recognized specifically the V. cholerae ompW gene was used. The optimized time and temperature conditions for th...

journal_title：Letters in applied microbiology

authors： Srisuk C,Chaivisuthangkura P,Rukpratanporn S,Longyant S,Sridulyakul P,Sithigorngul P

更新日期：2010-01-01 00:00:00

abstract：AIM:  This report describes the use of a six-gene multi-locus sequence analysis (MLSA) to correctly identify Vibrio strains of the Harveyi clade. METHODS AND RESULTS:  Vibrio isolates were characterized using a six housekeeping gene MLSA. The study provided evidence supporting: (i) a substantial number of reference st...

journal_title：Letters in applied microbiology

authors： Hoffmann M,Monday SR,Fischer M,Brown EW

更新日期：2012-02-01 00:00:00

abstract：AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...

journal_title：Letters in applied microbiology

authors： Carrasco L,Mena KD,Mota LC,Ortiz M,Behravesh CB,Gibbs SG,Bristol JR,Mayberry L,Cardenas VM

更新日期：2008-06-01 00:00:00

abstract：AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...

journal_title：Letters in applied microbiology

authors： Al Groosh D,Roudsari GB,Moles DR,Ready D,Noar JH,Pratten J

更新日期：2011-05-01 00:00:00

abstract：AIMS:The aim of this study was to explore the occurrence of methicillin-resistant staphylococci in a large urban public transport system. METHODS AND RESULTS:Samples were taken from hand rails, which passengers hold onto when they are standing. In total, 1400 swabs taken from 55 vehicles (trolleybuses, trams and buses...

journal_title：Letters in applied microbiology

authors： Stepanović S,Cirković I,Djukić S,Vuković D,Svabić-Vlahović M

更新日期：2008-10-01 00:00:00

abstract：AIMS:The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants. METHODS AND RESULTS:The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recomb...

journal_title：Letters in applied microbiology

authors： Alberto F,Navarro D,de Vries RP,Asther M,Record E

更新日期：2009-08-01 00:00:00

abstract：AIMS:  We evaluated risks associated with diaper changing in Finnish kindergartens where children were using either modern disposable paper or reusable cloth diapers. METHODS AND RESULTS:  We determined enteric micro-organisms and ammonia in diaper-changing rooms in four kindergartens in autumn and winter in the ambie...

journal_title：Letters in applied microbiology

authors： Vaattovaara PE,Kivimäenpää M,Vaattovaara P,Pasanen P,Heinonen-Tanski H

更新日期：2012-05-01 00:00:00

abstract：:Candida albicans is an opportunistic fungus, which causes vulvovaginal candidiasis (VVC). The aim of this study was to evaluate Mrr2 mutation and its expression levels and Candida drug resistance 1 (Cdr1) in C. albicans associated with fluconazole (FCA) resistance. We identified 80 isolates of C. albicans from 155 vag...

journal_title：Letters in applied microbiology

authors： Feng W,Yang J,Ji Y,Xi Z,Yang L,Zhu X,Ma Y

更新日期：2020-02-01 00:00:00

abstract：:A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...

journal_title：Letters in applied microbiology

authors： Naik MM,Khanolkar D,Dubey SK

更新日期：2013-02-01 00:00:00