Novel multiplex polymerase chain reaction primer set for identification of Lactococcus species.

abstract：:The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...

journal_title：Letters in applied microbiology

authors： Hébert L,Rincé I,Sanna C,Laugier C,Rincé A,Petry S

更新日期：2014-10-01 00:00:00

abstract：:A Coxiella sp. closely related to the agent of Q-fever, Coxiella burnetii, has been associated with mortalities in redclaw crayfish, (Cherax quadricarinatus), in farms and experimental facilities for three decades. Limited sequence data including 16S rRNA have placed the rickettsial species as a new species, candidatu...

journal_title：Letters in applied microbiology

authors： Ruth Elliman J,Owens L

更新日期：2020-09-01 00:00:00

abstract：:Condensed tannins (CTs), which extracted from yew leaves, tilia flower and black locust leaves, were examined for their antimicrobial in vitro activity against avian pathogenic Escherichia coli (APEC). Past research demonstrated that CTs which contain procyanidins and prodelphinidins that could inhibit the growth of a...

journal_title：Letters in applied microbiology

authors： Dakheel MM,Alkandari FAH,Mueller-Harvey I,Woodward MJ,Rymer C

更新日期：2020-03-01 00:00:00

abstract：UNLABELLED:Healthcare-associated infections (HCAI) affect 5-10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such...

journal_title：Letters in applied microbiology

authors： Galvin S,Cahill O,O'Connor N,Cafolla AA,Daniels S,Humphreys H

更新日期：2013-08-01 00:00:00

abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...

journal_title：Letters in applied microbiology

authors： Auvray F,Lecureuil C,Dilasser F,Taché J,Derzelle S

更新日期：2009-05-01 00:00:00

abstract：AIM:To compare the incidence of antimicrobial resistance among Salmonella serotypes isolated in a pig slaughterhouse in Zaragoza (Spain) during 1993 and 2001. METHODS AND RESULTS:A total of 168 isolates representing 10 serotypes were examined by disc diffusion method using 17 antibiotics. Data showed that the majority...

journal_title：Letters in applied microbiology

authors： Agustín AI,Carramiñana JJ,Rota C,Herrera A

更新日期：2005-01-01 00:00:00

abstract：AIM:To stimulate poly-beta-hydroxybutyrate (PHB) accumulation in Synechocystis sp. PCC 6803 by manipulating culture conditions. METHODS AND RESULTS:Stationary phase cultures of Synechocystis sp. PCC 6803 were subjected to N- and P-deficiency, chemoheterotrophy and limitations of gas-exchange. Enhanced PHB accumulation...

journal_title：Letters in applied microbiology

authors： Panda B,Mallick N

更新日期：2007-02-01 00:00:00

abstract：AIM:The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. METHODS AND RESULTS:The sakacin P and sakacin G structural genes were identified in Lact. curv...

journal_title：Letters in applied microbiology

authors： Dortu C,Huch M,Holzapfel WH,Franz CM,Thonart P

更新日期：2008-12-01 00:00:00

abstract：AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...

journal_title：Letters in applied microbiology

authors： La Ragione RM,Narbad A,Gasson MJ,Woodward MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:Haloarchaeal proteases function optimally in high salt (low water activity); thus, they offer an advantage over the nonhalophilic counterparts as biocatalysts for protease-catalysed peptide synthesis. The haloalkaliphilic archaeon Natrialba magadii secretes a solvent-tolerant protease, Nep (Natrialba magadii extra...

journal_title：Letters in applied microbiology

authors： Ruiz DM,Iannuci NB,Cascone O,De Castro RE

更新日期：2010-12-01 00:00:00

abstract：AIM:To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. METHODS AND RESULTS:Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and...

journal_title：Letters in applied microbiology

authors： Velliou EG,Van Derlinden E,Cappuyns AM,Aerts D,Nikolaidou E,Geeraerd AH,Devlieghere F,Van Impe JF

更新日期：2011-02-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...

journal_title：Letters in applied microbiology

authors： Tanaro JD,Pianciola LA,D'Astek BA,Piaggio MC,Mazzeo ML,Zolezzi G,Rivas M

更新日期：2018-06-01 00:00:00

abstract：:A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

journal_title：Letters in applied microbiology

authors： Steinhauserova I,Fojtikova K,Klimes J

更新日期：2000-09-01 00:00:00

abstract：AIMS:We report the analysis of genetic determinants conferring resistance to pristinamycin in Staphylococcus epidermidis strains and epidemiology typing of these strains by pulsed-field gel electrophoresis. METHODS AND RESULTS:Staphylococcus epidermidis (346 isolates) were searched for strains with pristinamycin resis...

journal_title：Letters in applied microbiology

authors： Achour W,Bouchami O,Galopin S,Leclercq R,Ben Hassen A

更新日期：2008-03-01 00:00:00

abstract：AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...

journal_title：Letters in applied microbiology

authors： Al Groosh D,Roudsari GB,Moles DR,Ready D,Noar JH,Pratten J

更新日期：2011-05-01 00:00:00

abstract：UNLABELLED:A : Comparison of two commercial miniaturized rapid systems for the identification of Ralstonia pickettii strains. METHODS AND RESULTS:Varying identification results were encountered using the bioMérieux API NE system and the Remel IDS RapID NF Plus commercial systems for R. pickettii. To compare these two ...

journal_title：Letters in applied microbiology

authors： Adley CC,Saieb FM

更新日期：2005-01-01 00:00:00

abstract：AIMS:This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. METHODS AND RESULTS:A total of 285 stool samples were inoculated onto Chr...

journal_title：Letters in applied microbiology

authors： Asir K,Wilkinson K,Perry JD,Reed RH,Gould FK

更新日期：2009-02-01 00:00:00

abstract：:Results of biocide efficacy testing on laboratory-generated microbial biofilms containing Legionella bozemanii is presented. These show that chlorine is effective at recommended concentrations in cooling towers; at least 48 h contact between free chlorine and the biofilm is necessary. Of five commercial biocides teste...

journal_title：Letters in applied microbiology

authors： Green PN

更新日期：1993-10-01 00:00:00

abstract：:A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected m...

journal_title：Letters in applied microbiology

authors： Candlish AA,Smith JE,Stimson WH

更新日期：1990-04-01 00:00:00

abstract：:The present study demonstrates the siderophore production of two strains of Pseudomonas fluorescens and two of Ps. chlororaphis. The antimicrobial activities of these strains were studied against Salmonella typhimurium, Staphylococcus aureus, Fusarium culmorum, F. oxysporum and Aspergillus niger. Despite equal siderop...

journal_title：Letters in applied microbiology

authors： Laine MH,Karwoski MT,Raaska LB,Mattila-Sandholm TM

更新日期：1996-03-01 00:00:00

abstract：UNLABELLED:In this study, we identified five strains isolated from soil and sediments able to degrade kraft lignin, aromatic dyes and lignin derivatives. Using 16S rRNA gene sequencing, the isolates were identified as Serratia sp. JHT01, Serratia liquefacien PT01, Pseudomonas chlororaphis PT02, Stenotrophomonas maltoph...

journal_title：Letters in applied microbiology

authors： Tian JH,Pourcher AM,Peu P

更新日期：2016-07-01 00:00:00

abstract：AIMS:  The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS:  A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the ...

journal_title：Letters in applied microbiology

authors： Ruiz LM,Castro M,Barriga A,Jerez CA,Guiliani N

更新日期：2012-02-01 00:00:00

abstract：AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...

journal_title：Letters in applied microbiology

authors： Serio A,Chiarini M,Tettamanti E,Paparella A

更新日期：2010-08-01 00:00:00

abstract：AIM:To test the efficacy of a chemical (chlorous acid) for reducing the numbers of viable Alicyclobacillus acidoterrestris spores in laboratory media and on apples. METHODS AND RESULTS:Alicyclobacillus acidoterrestris spores in aqueous suspension and on apple surfaces of four different cultivars were treated with 268 ...

journal_title：Letters in applied microbiology

authors： Lee SY,Ryu SR,Kang DH

更新日期：2010-08-01 00:00:00

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

journal_title：Letters in applied microbiology

authors： Buddie AG,Bridge PD,Kelley J,Ryan MJ

更新日期：2011-01-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：:Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluat...

journal_title：Letters in applied microbiology

authors： Miao YJ,Xiong GT,Bai MY,Ge Y,Wu ZF

更新日期：2018-05-01 00:00:00

abstract：:Recent studies have shown that Salmonella shedding status affects sows' microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: (a) to evaluate the persistence, up to the fattening period, of the previously measured link ...

journal_title：Letters in applied microbiology

authors： Larivière-Gauthier G,Thibodeau A,Yergeau É,Fravalo P

更新日期：2021-02-01 00:00:00

abstract：:Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...

journal_title：Letters in applied microbiology

authors： Yao KY,Zhang TZ,Wang HF,Liu JX

更新日期：2018-12-01 00:00:00