Aflatoxin monoclonals: academic development to commercial production.

Abstract:

:A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected mAb was then developed in direct competitive ELISA and immunoaffinity column chromatography methods for aflatoxin detection in foods and feeds. Both assays are rapid, sensitive, specific and require only the minimum of sample preparation. Both immunological assays have now been commercialized and are produced in convenient ready-made kit formats.

journal_name

Lett Appl Microbiol

authors

Candlish AA,Smith JE,Stimson WH

doi

10.1111/j.1472-765x.1990.tb00106.x

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

167-9

issue

4

eissn

0266-8254

issn

1472-765X

journal_volume

10

pub_type

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