A simple artificial urine for the growth of urinary pathogens.

abstract：:The specific detection and enumeration of Lactobacillus brevis LB62, Carnobacterium divergens V14 and Carnobacterium piscicola VI were studied by in situ hybridization-flow cytometry. The method was performed on the exponential growth phase with three probes targeting 16S rRNA labelled with fluorescein isothicyanate (...

journal_title：Letters in applied microbiology

authors： Connil N,Dousset X,Onno B,Pilet MF,Breuil MF,Montel MC

更新日期：1998-11-01 00:00:00

abstract：:This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...

journal_title：Letters in applied microbiology

authors： Willinger B,Manafi M

更新日期：1995-05-01 00:00:00

abstract：:A native isolate Lactobacillus farciminis MD isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. Also, the efficacy of Lact. farciminis MD as a sensitive strain for antibiotic assay was established...

journal_title：Letters in applied microbiology

authors： Halami PM,Chandrashekar A,Nand K

更新日期：2000-03-01 00:00:00

abstract：AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were ...

journal_title：Letters in applied microbiology

authors： Ramabu SS,Boxall NS,Madie P,Fenwick SG

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:The objective of this study was to evaluate the effect of different caffeine concentrations (0-4%) on (i) lag phase prior to growth, (ii) growth rates and (iii) ochratoxin A (OTA) production by strains from the Aspergillus section Circumdati and Aspergillus section Nigri groups, isolated from coffee, when gr...

journal_title：Letters in applied microbiology

authors： Akbar A,Medina A,Magan N

更新日期：2016-07-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：UNLABELLED:Industrial biotechnology uses microbiological cells to produce a wide range of products. While the organisms in question are well understood regarding their genetic and molecular properties, less is known about their mechanical properties. Previous work has established a testing procedure for single Saccharo...

journal_title：Letters in applied microbiology

authors： Overbeck A,Kampen I,Kwade A

更新日期：2015-10-01 00:00:00

abstract：:The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...

journal_title：Letters in applied microbiology

authors： Hébert L,Rincé I,Sanna C,Laugier C,Rincé A,Petry S

更新日期：2014-10-01 00:00:00

abstract：AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...

journal_title：Letters in applied microbiology

authors： Zhang HY,Yang Q,Wang G,Shang FD

更新日期：2009-03-01 00:00:00

abstract：AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

journal_title：Letters in applied microbiology

authors： Chowdhury B,Adak M,Bose SK

更新日期：2003-01-01 00:00:00

abstract：AIMS:To investigate the effect of molasses concentration, initial pH of molasses medium, and inoculum's size to maximize ethanol and minimize methanol, fusel alcohols, acetic acid and aldehydes in the fermentation mash in industrial fermentors. METHODS AND RESULTS:Initial studies to optimize temperature, nitrogen sour...

journal_title：Letters in applied microbiology

authors： Arshad M,Khan ZM,Khalil-ur-Rehman,Shah FA,Rajoka MI

更新日期：2008-11-01 00:00:00

abstract：:A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic Europ...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Rasmussen OF

更新日期：1994-11-01 00:00:00

abstract：:The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...

journal_title：Letters in applied microbiology

authors： Flahaut S,Laplace JM,Frère J,Auffray Y

更新日期：1998-04-01 00:00:00

abstract：AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

journal_title：Letters in applied microbiology

authors： Zanetti F,De Luca G,Tarlazzi P,Stampi S

更新日期：2003-01-01 00:00:00

abstract：:Erwinia amylovora bacteria cause fire blight disease, which affects apple and pear production worldwide. The Erw. amylovora pyrC gene encodes a predicted dihydroorotase enzyme involved in pyrimidine biosynthesis. Here, we discovered that the Erw. amylovora pyrC244::Tn5 mutant was a uracil auxotroph. Unexpectedly, the ...

journal_title：Letters in applied microbiology

authors： Ramos LS,Sinn JP,Lehman BL,Pfeufer EE,Peter KA,McNellis TW

更新日期：2015-06-01 00:00:00

abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...

journal_title：Letters in applied microbiology

authors： Korem M,Goldberg NS,Cahan A,Cohen MJ,Nissenbaum I,Moses AE

更新日期：2018-07-01 00:00:00

abstract：:Washed Escherichia coli ATCC11775 cells were killed by (-)-epigallocatechin (EGC) in the presence of a non-lethal concentration of Cu2+ (1 mumol l-1) without additional H2O2, but not by (-)-epicatechin (EC). EGC alone (< 0.1 mmol l-1) did not reduce the viability of the cells. The survival curve obtained in the presen...

journal_title：Letters in applied microbiology

authors： Kimura T,Hoshino N,Yamaji A,Hayakawa F,Ando T

更新日期：1998-12-01 00:00:00

abstract：AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...

journal_title：Letters in applied microbiology

authors： Igarashi T,Yano Y,Yamamoto A,Sasa R,Goto N

更新日期：2001-06-01 00:00:00

abstract：AIMS:Nine sets of PCR primers targeting Salmonella were evaluated for their specificity with pure cultures of intestinal-associated bacteria prior to their application to Salmonella detection in faecal samples. METHODS AND RESULTS:Gene targets of PCR primers included: 16S rDNA, a Salmonella pathogenicity island I viru...

journal_title：Letters in applied microbiology

authors： Ziemer CJ,Steadham SR

更新日期：2003-01-01 00:00:00

abstract：:Anti-MRSA activity of sophoraflavanone G (SFG) and synergism between SFG and antibacterial agents against MRSA (methicillin-resistant Staphylococcus aureus) were evaluated by means of Minimal Inhibitory Concentrations (MIC). The MICs of SFG against 27 strains of MRSA ranged from 3.13 to 6.25 micrograms ml-1. Synergism...

journal_title：Letters in applied microbiology

authors： Sakagami Y,Mimura M,Kajimura K,Yokoyama H,Linuma M,Tanaka T,Ohyama M

更新日期：1998-08-01 00:00:00

abstract：AIM:To examine whether isolates of the entomopathogenic fungus Beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. METHODS AND RESULTS:Primers for the traditional ITS1-5.8S-ITS2 region and two mi...

journal_title：Letters in applied microbiology

authors： Kouvelis VN,Ghikas DV,Edgington S,Typas MA,Moore D

更新日期：2008-03-01 00:00:00

abstract：AIMS:Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. METHODS AND RESULTS:Direct-cu...

journal_title：Letters in applied microbiology

authors： Nishino N,Li Y,Wang C,Parvin S

更新日期：2012-03-01 00:00:00

abstract：:The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...

journal_title：Letters in applied microbiology

authors： Quang Le H,Suffredini E,Tien Pham D,Kim To A,De Medici D

更新日期：2018-11-01 00:00:00

abstract：:Live shellfish samples (120) were collected from nine littoral sites in Brittany (western France). They were screened for Listeria spp. and a count of faecal coliforms was carried out. Analysis of the results revealed Listeria spp. in 55% of samples, a much higher rate than the previous, infrequent, recorded data. Fur...

journal_title：Letters in applied microbiology

authors： Monfort P,Minet J,Rocourt J,Piclet G,Cormier M

更新日期：1998-03-01 00:00:00

abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：AIMS:To evaluate a new dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) assay for detection of six sexually transmitted pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum and Trichomonas vaginalis. METHO...

journal_title：Letters in applied microbiology

authors： Horii T,Ohtsuka H,Osaki M,Ohkuni H

更新日期：2009-07-01 00:00:00

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：:Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of ...

journal_title：Letters in applied microbiology

authors： Abdel-Kareem MM,Zohri AA

更新日期：2018-11-01 00:00:00

abstract：AIMS:In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human...

journal_title：Letters in applied microbiology

authors： Plutzer J,Törökné A,Karanis P

更新日期：2010-01-01 00:00:00

abstract：:The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

journal_title：Letters in applied microbiology

authors： Park SH,Kim WJ,Kang DH

更新日期：2018-08-01 00:00:00