Abstract：
AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were assessed. METHODS AND RESULTS:Pooled swab samples were obtained from pallets, crates, wheels of trucks, tractors and forklifts, truck beds, and from drivers' and catchers' boots. After enrichment in Bolton's broth Campylobacter were recovered on modified blood-free Campylobacter selective agar (mCCDA). Isolates were identified using tests for phenotypic and biochemical characteristics. Of the 209 samples collected, 53% were positive for C. jejuni, with all fomites positive except tractor wheels. Pallets had the highest contamination rate at 75%. More than 50% of catchers' boots, drivers' boots, crates and truck wheels were positive. Forty-seven per cent and 31% of truck beds and forklift wheels, respectively, were contaminated. The proportion of contaminated fomites did not change significantly during the day. CONCLUSIONS:This study has identified trucks, forklifts, pallets, crates, drivers' and catchers' boots as potential sources of C. jejuni for broilers. SIGNIFICANCE AND IMPACT OF THE STUDY:Campylobacter jejuni contamination of broiler processing plant fomites was found to be extensive ranging from 31% for truck beds to 75% for pallets. The proportion of contaminated fomites was observed to be similar throughout the day. The impact of contaminated fomites as sources of colonization of broilers with C. jejuni is discussed.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Ramabu SS,Boxall NS,Madie P,Fenwick SGdoi
10.1111/j.1472-765X.2004.01573.xsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
252-6issue
3eissn
0266-8254issn
1472-765Xpii
LAM1573journal_volume
39pub_type
杂志文章abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...
journal_title：Letters in applied microbiology
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doi：10.1111/j.1472-765x.1994.tb00916.x
更新日期：1994-08-01 00:00:00
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doi：10.1046/j.1472-765x.1997.00207.x
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pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00411.x
更新日期：1998-09-01 00:00:00
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journal_title：Letters in applied microbiology
pub_type： 杂志文章
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journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.1992.tb00744.x
更新日期：1992-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1472-765x.2002.01108.x
更新日期：2002-01-01 00:00:00
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journal_title：Letters in applied microbiology
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Letters in applied microbiology
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journal_title：Letters in applied microbiology
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journal_title：Letters in applied microbiology
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journal_title：Letters in applied microbiology
pub_type： 杂志文章
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journal_title：Letters in applied microbiology
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journal_title：Letters in applied microbiology
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journal_title：Letters in applied microbiology
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journal_title：Letters in applied microbiology
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更新日期：2011-09-01 00:00:00
abstract：:A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00355.x
更新日期：1998-05-01 00:00:00
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journal_title：Letters in applied microbiology
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更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
