An assay to screen bacterial adhesion to mucus biomolecules.

abstract：AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...

journal_title：Letters in applied microbiology

authors： Hansen CR,Khatiwara A,Ziprin R,Kwon YM

更新日期：2007-12-01 00:00:00

abstract：:A viable but non-culturable (VBNC) bacterial state was originally detected in studies in environmental microbiology. In particular, this state has been demonstrated for a number of human pathogens (Escherichia coli, Salmonella enteritidis, Vibrio cholerae, Legionella pneumophila and Campylobacter jejuni). The presence...

journal_title：Letters in applied microbiology

authors： Besnard V,Federighi M,Cappelier JM

更新日期：2000-07-01 00:00:00

abstract：AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...

journal_title：Letters in applied microbiology

authors： Gudiña EJ,Rocha V,Teixeira JA,Rodrigues LR

更新日期：2010-04-01 00:00:00

abstract：AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...

journal_title：Letters in applied microbiology

authors： Rogers JV,Richter WR,Shaw MQ,Choi YW

更新日期：2008-10-01 00:00:00

abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...

journal_title：Letters in applied microbiology

authors： Fitzgerald AC,Edrington TS,Looper ML,Callaway TR,Genovese KJ,Bischoff KM,McReynolds JL,Thomas JD,Anderson RC,Nisbet DJ

更新日期：2003-01-01 00:00:00

abstract：AIMS:To investigate the impact of acetaldehyde- and pyruvic acid-bound sulphur dioxide on wine lactic acid bacteria (LAB). METHODS AND RESULTS:Growth studies were performed where Oenococcus oeni, Pediococcus parvulus, Ped. damnosus and Lactobacillus hilgardii were inoculated into media containing various concentration...

journal_title：Letters in applied microbiology

authors： Wells A,Osborne JP

更新日期：2012-03-01 00:00:00

abstract：AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

journal_title：Letters in applied microbiology

authors： Chang PK,Hua SS

更新日期：2007-02-01 00:00:00

abstract：:To enhance the overall expression level of lipase isozymes which catalyse the same reaction in Pichia pastoris through co-expression of isozymes from different sources; several types of co-expression ways were constructed to determine the co-expression efficiencies of lipase isozymes in P. pastoris. The results showed...

journal_title：Letters in applied microbiology

authors： Cai H,Zhang T,Zhao M,Mao J,Cai C,Feng F

更新日期：2017-10-01 00:00:00

abstract：:Candida species are increasingly important fungal pathogens. The reaction of rat alveolar macrophages (AM) to Candida albicans was compared with that to C. glabrata and C. krusei. Phagocytosis of C. glabrata was similar to that of C. albicans, but significantly slower for C. krusei due to reduced attachment. After ops...

journal_title：Letters in applied microbiology

authors： Nessa K,Johansson A,Jarstrand C,Camner P

更新日期：1997-09-01 00:00:00

abstract：AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...

journal_title：Letters in applied microbiology

authors： Tsuchiya C,Sakata T,Sugita H

更新日期：2008-01-01 00:00:00

abstract：UNLABELLED:The aim of this study was to assess the genetic diversity of Vibrio cholerae isolated from 2012 and 2013 outbreaks in Iran, with regard to their virulence properties. A total of 20 V. cholerae strains were collected from Sistan-Baluchestan province of Iran during 2012 and 2013 outbreaks. Hybridization assays...

journal_title：Letters in applied microbiology

authors： Bakhshi B

更新日期：2016-06-01 00:00:00

abstract：AIMS:To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. METHODS AND RESULTS:The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons we...

journal_title：Letters in applied microbiology

authors： Wang HY,Gao YB,Fan QW,Xu Y

更新日期：2011-08-01 00:00:00

abstract：:Washed Escherichia coli ATCC11775 cells were killed by (-)-epigallocatechin (EGC) in the presence of a non-lethal concentration of Cu2+ (1 mumol l-1) without additional H2O2, but not by (-)-epicatechin (EC). EGC alone (< 0.1 mmol l-1) did not reduce the viability of the cells. The survival curve obtained in the presen...

journal_title：Letters in applied microbiology

authors： Kimura T,Hoshino N,Yamaji A,Hayakawa F,Ando T

更新日期：1998-12-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：AIM:The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. METHODS AND RESULTS:The sakacin P and sakacin G structural genes were identified in Lact. curv...

journal_title：Letters in applied microbiology

authors： Dortu C,Huch M,Holzapfel WH,Franz CM,Thonart P

更新日期：2008-12-01 00:00:00

abstract：AIM:To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. METHODS AND RESULTS:Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and...

journal_title：Letters in applied microbiology

authors： Velliou EG,Van Derlinden E,Cappuyns AM,Aerts D,Nikolaidou E,Geeraerd AH,Devlieghere F,Van Impe JF

更新日期：2011-02-01 00:00:00

abstract：AIM:The main aim of this study was to investigate a possible connection between the Salmonella content in sewage sludge and human cases of salmonellosis. An additional aim was to survey the antimicrobial resistance situation in Salmonella isolated from Swedish sewage sludge. METHODS AND RESULTS:The Salmonella strains ...

journal_title：Letters in applied microbiology

authors： Sahlström L,de Jong B,Aspan A

更新日期：2006-07-01 00:00:00

abstract：:Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface mater...

journal_title：Letters in applied microbiology

authors： Redfern J,Verran J

更新日期：2017-04-01 00:00:00

abstract：:Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a si...

journal_title：Letters in applied microbiology

authors： Elvin S,Bingham AH

更新日期：1991-02-01 00:00:00

abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

journal_title：Letters in applied microbiology

authors： Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

更新日期：2018-09-01 00:00:00

abstract：AIM:Combination of immunomagnetic separation (IMS) and lateral flow device (LFD) assays for the development of a sensitive, rapid, on-site methodology that enables concentration and detection of Bacillus anthracis spores in complex samples. METHODS AND RESULTS:The data presents the development of an optimized, 30 min,...

journal_title：Letters in applied microbiology

authors： Fisher M,Atiya-Nasagi Y,Simon I,Gordin M,Mechaly A,Yitzhaki S

更新日期：2009-04-01 00:00:00

abstract：:Shigella spp. are water-borne pathogens responsible for mild to severe cases bacilli dysentery all around the world known as Shigellosis. The progressively increasing of antibiotic resistance among Shigella calls for developing and establishing novel alternative therapeutic methods. The present study aimed to evaluate...

journal_title：Letters in applied microbiology

authors： Shahin K,Zhang L,Bao H,Hedayatkhah A,Soleimani-Delfan A,Komijani M,He T,Barazandeh M,Mansoorianfar M,Bouzari M,Wang R

更新日期：2020-10-18 00:00:00

abstract：:Pyrimidine synthesis in the food spoilage agent Burkholderia cepacia ATCC 25416 was investigated. The five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in B. cepacia ATCC 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. The in vitro regulation of a...

journal_title：Letters in applied microbiology

authors： Li K,West TP

更新日期：1995-11-01 00:00:00

abstract：AIMS:Our goal was to develop a mathematical kinetic model to predict the sporicidal activity of glutaraldehyde, which is an active ingredient frequently used in commercial products employed for liquid disinfection and decontamination. METHODS AND RESULTS:We used our previously published data on spore inactivation by g...

journal_title：Letters in applied microbiology

authors： Retta SM,Sagripanti JL

更新日期：2008-05-01 00:00:00

abstract：:The purpose of this investigation was to evaluate intracanal bacterial reduction by cryotreatment using a dental instrument equipped with a duct and connected to a cryogenic fluid source. A total of 86 roots were infected with Enterococcus faecalis and incubated. After incubation, the contaminated roots were divided i...

journal_title：Letters in applied microbiology

authors： Mandras N,Allizond V,Bianco A,Banche G,Roana J,Piazza L,Viale P,Cuffini AM

更新日期：2013-02-01 00:00:00

abstract：AIMS:To investigate the effect of molasses concentration, initial pH of molasses medium, and inoculum's size to maximize ethanol and minimize methanol, fusel alcohols, acetic acid and aldehydes in the fermentation mash in industrial fermentors. METHODS AND RESULTS:Initial studies to optimize temperature, nitrogen sour...

journal_title：Letters in applied microbiology

authors： Arshad M,Khan ZM,Khalil-ur-Rehman,Shah FA,Rajoka MI

更新日期：2008-11-01 00:00:00

abstract：:A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

journal_title：Letters in applied microbiology

authors： Steinhauserova I,Fojtikova K,Klimes J

更新日期：2000-09-01 00:00:00

abstract：AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

journal_title：Letters in applied microbiology

authors： Eyigor A,Carli KT,Unal CB

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

journal_title：Letters in applied microbiology

authors： Burow LC,Gobius KS,Vanselow BA,Klieve AV

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...

journal_title：Letters in applied microbiology

authors： Richard C,Brillet A,Pilet MF,Prévost H,Drider D

更新日期：2003-01-01 00:00:00