Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury.

abstract：AIMS:To investigate the susceptibility of 221 Listeria spp. (86 Listeria monocytogenes, 41 Listeria innocua and 94 Listeria seeligeri-Listeria welshimeri-Listeria ivanovii) isolated from catfish fillets and processing environment to 15 antibiotics. METHODS AND RESULTS:Listeria isolates were analysed by disc-diffusion ...

journal_title：Letters in applied microbiology

authors： Chen BY,Pyla R,Kim TJ,Silva JL,Jung YS

更新日期：2010-06-01 00:00:00

abstract：:The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast ex...

journal_title：Letters in applied microbiology

authors： Rowan NJ,MacGregor SJ,Anderson JG,Fouracre RA,Farish O

更新日期：2000-08-01 00:00:00

abstract：AIMS:To evaluate the genetic diversity within the species Paenibacillus polymyxa. METHODS:Southern hybridization was performed on 102 strains of P. polymyxa using DNA from the phage IPy1 as a probe. RESULTS:All 102 strains hybridized to phage IPy1 DNA. Data from different hybridization patterns obtained were used to ...

journal_title：Letters in applied microbiology

authors： Santos SC,Rodrigues Coelho MR,Seldin L

更新日期：2002-01-01 00:00:00

abstract：AIMS:The study was aimed to evaluate the Mycobacterium leprae recombinant early secreted antigenic target-6 (rESAT-6) for its serological performance in leprosy patients. METHODS AND RESULTS:Employing enzyme-linked immunosorbent assay (ELISA), serum samples were tested for prevalence of immunoglobulin G antibodies aga...

journal_title：Letters in applied microbiology

authors： Parkash O,Pandey R,Kumar A,Kumar A

更新日期：2007-05-01 00:00:00

abstract：AIM:To evaluate the presence of thermotolerant Campylobacter spp. in stray cats in southern Italy. METHODS AND RESULTS:One hundred and thirteen animals were trapped in two different environments (urban area, harbour area) of the city of Naples. From each cat, rectal swabs were collected. The samples were processed in ...

journal_title：Letters in applied microbiology

authors： Gargiulo A,Rinaldi L,D'Angelo L,Dipineto L,Borrelli L,Fioretti A,Menna LF

更新日期：2008-02-01 00:00:00

abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

journal_title：Letters in applied microbiology

authors： McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

更新日期：2005-01-01 00:00:00

abstract：AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...

journal_title：Letters in applied microbiology

authors： Chen JC,Lin ZH,Ma XX

更新日期：2003-01-01 00:00:00

abstract：:Leclercia adecarboxylata, a Gram-negative bacillus of family Enterobacteriaceae, is an uncommonly identified pathogen isolated from environmental and clinical specimens. Most of the human infections are polymicrobial and commonly occur in immunocompromised hosts, although nosocomial infections in immunocompetent hosts...

journal_title：Letters in applied microbiology

authors： Choudhary M,Choudhary BK,Bhoyar S,Kale SB,Chaudhari SP,Bera BC,Jain A,Barbuddhe SB

更新日期：2018-01-01 00:00:00

abstract：AIMS:  We evaluated risks associated with diaper changing in Finnish kindergartens where children were using either modern disposable paper or reusable cloth diapers. METHODS AND RESULTS:  We determined enteric micro-organisms and ammonia in diaper-changing rooms in four kindergartens in autumn and winter in the ambie...

journal_title：Letters in applied microbiology

authors： Vaattovaara PE,Kivimäenpää M,Vaattovaara P,Pasanen P,Heinonen-Tanski H

更新日期：2012-05-01 00:00:00

abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...

journal_title：Letters in applied microbiology

authors： Fitzgerald AC,Edrington TS,Looper ML,Callaway TR,Genovese KJ,Bischoff KM,McReynolds JL,Thomas JD,Anderson RC,Nisbet DJ

更新日期：2003-01-01 00:00:00

abstract：:Accumulation of proline and glycine betaine occurs via three transport systems in Staphylococcus aureus (Pourkomailian and Booth 1992, 1994). A 35 kDa membrane protein was identified to be missing from a mutant, BP108, which lacks activity of the low affinity osmolyte transport system. The loss in activity of the low ...

journal_title：Letters in applied microbiology

authors： Pourkomaillan B

更新日期：1998-02-01 00:00:00

abstract：:A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...

journal_title：Letters in applied microbiology

authors： Naik MM,Khanolkar D,Dubey SK

更新日期：2013-02-01 00:00:00

abstract：AIMS:Rapid detection of pathogenic Yersinia enterocolitica isolates by using antisera raised against recombinant attachment-invasion locus (Ail) protein. METHODS AND RESULTS:The complete gene (471 bp) encoding for the Ail protein was amplified by PCR and cloned in pQE 30 UA vector. The recombinant clones were selected...

journal_title：Letters in applied microbiology

authors： Balakrishna K,Murali HS,Batra HV

更新日期：2010-02-01 00:00:00

abstract：AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....

journal_title：Letters in applied microbiology

authors： Aguilar-Uscanga MG,Garcia-Alvarado Y,Gomez-Rodriguez J,Phister T,Delia ML,Strehaiano P

更新日期：2011-08-01 00:00:00

abstract：AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To...

journal_title：Letters in applied microbiology

authors： Yin Y,Liu X,Ma Z

更新日期：2009-06-01 00:00:00

abstract：:Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...

journal_title：Letters in applied microbiology

authors： Fenlon DR,Stewart T,Donachie W

更新日期：1995-01-01 00:00:00

abstract：:While the beta-glucuronidase activity of intact cells of Clostridium perfringens was higher in 0.95% sodium chloride (NaCl) than that in 0, 0.1 or 0.5%, that of Escherichia coli was higher in 0.1% NaCl than that in 0, 0.5 or 0.95% NaCl in 0.1 mol l-1 KH2PO4. However, the enzyme activity of both species of intact cells...

journal_title：Letters in applied microbiology

authors： Fujisawa T,Aikawa K,Takahashi T,Yamai S

更新日期：2000-09-01 00:00:00

abstract：AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...

journal_title：Letters in applied microbiology

authors： Zhang HY,Yang Q,Wang G,Shang FD

更新日期：2009-03-01 00:00:00

abstract：AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

journal_title：Letters in applied microbiology

authors： Rogers JV,Ducatte GR,Choi YW,Early PC

更新日期：2006-11-01 00:00:00

abstract：AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...

journal_title：Letters in applied microbiology

authors： Zhang GW,Kotiw M,Daggard G

更新日期：2002-01-01 00:00:00

abstract：AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

journal_title：Letters in applied microbiology

authors： Chang PK,Hua SS

更新日期：2007-02-01 00:00:00

abstract：AIMS:Bacillus halodurans C-125 is a Gram-positive bacterium that was the first alkaliphilic species to have its genome completely sequenced. Despite its many years as a model for alkaliphily and source of industrially important enzymes, genetic manipulation of B. halodurans C-125 remains difficult, and therefore, we so...

journal_title：Letters in applied microbiology

authors： Wallace JG,Breaker RR

更新日期：2011-04-01 00:00:00

abstract：AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were ...

journal_title：Letters in applied microbiology

authors： Ramabu SS,Boxall NS,Madie P,Fenwick SG

更新日期：2004-01-01 00:00:00

abstract：:Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. cavi...

journal_title：Letters in applied microbiology

authors： Hossain S,Wickramanayake MVKS,Dahanayake PS,Heo GJ

更新日期：2020-03-01 00:00:00

abstract：AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

journal_title：Letters in applied microbiology

authors： Narciso JA,Ference CM,Ritenour MA,Widmer WW

更新日期：2012-02-01 00:00:00

abstract：:Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced...

journal_title：Letters in applied microbiology

authors： Winters DK,O'Leary AE,Slavik MF

更新日期：1998-09-01 00:00:00

abstract：AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently an...

journal_title：Letters in applied microbiology

authors： Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GS

更新日期：2002-01-01 00:00:00

abstract：:The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...

journal_title：Letters in applied microbiology

authors： Coutinho HL,Handley BA,Kay HE,Stevenson L,Beringer JE

更新日期：1993-12-01 00:00:00

abstract：UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...

journal_title：Letters in applied microbiology

authors： Weigold P,Ruecker A,Jochmann M,Osorio Barajas XL,Lege S,Zwiener C,Kappler A,Behrens S

更新日期：2015-10-01 00:00:00

abstract：UNLABELLED:Palm wine produced traditionally and consumed by many people in the South-West of Burkina Faso is subject to alteration. In this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. We monitored biochemical characteris...

journal_title：Letters in applied microbiology

authors： Tapsoba F,Savadogo A,Legras JL,Zongo C,Traore AS

更新日期：2016-10-01 00:00:00