Effect of copper hydroxide sprays for citrus canker control on wild-type Escherichia coli.

Abstract:

AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS:  Studies were performed in vitro with bacteria grown in broth and then subjected to field spray copper hydroxide concentrations in the broth and on citrus leaves. Escherichia coli exposed to copper hydroxide in vitro were eradicated from the broth within 6-8 h depending on the broth pH. Even with near neutral pH (7·2), cells began to die immediately after exposure to copper. No E. coli survived on leaf surfaces sprayed with copper. CONCLUSIONS:  Copper field sprays mixed with water that may contain E. coli can help eliminate E. coli from plant surfaces. SIGNIFICANCE AND IMPACT OF THE STUDY:  HACCP mandates are becoming more restrictive because of the increased illness resulting from food pathogens on fresh produce. Use of potable water in fields, a proposed mandate, is not feasible for large grove owners. These data show that copper sprays aimed at reducing citrus canker also affect E. coli and may help to ease water quality mandates.

journal_name

Lett Appl Microbiol

authors

Narciso JA,Ference CM,Ritenour MA,Widmer WW

doi

10.1111/j.1472-765X.2011.03179.x

subject

Has Abstract

pub_date

2012-02-01 00:00:00

pages

108-11

issue

2

eissn

0266-8254

issn

1472-765X

journal_volume

54

pub_type

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